Cargando…
Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications
Veillonella atypica is a bacterium that is present in the gut and the oral cavity of mammals and plays diverse roles in different niches. A recent study demonstrated that Veillonella is highly associated with marathon running and approved that V. atypica gavage improves treadmill run time in mice, r...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324755/ https://www.ncbi.nlm.nih.gov/pubmed/32655519 http://dx.doi.org/10.3389/fmicb.2020.01219 |
_version_ | 1783552009008316416 |
---|---|
author | Han, Maozhen Liu, Gang Chen, Yajun Wang, Dong Zhang, Yan |
author_facet | Han, Maozhen Liu, Gang Chen, Yajun Wang, Dong Zhang, Yan |
author_sort | Han, Maozhen |
collection | PubMed |
description | Veillonella atypica is a bacterium that is present in the gut and the oral cavity of mammals and plays diverse roles in different niches. A recent study demonstrated that Veillonella is highly associated with marathon running and approved that V. atypica gavage improves treadmill run time in mice, revealing that V. atypica has a high biotechnological potential in improving athlete performance. However, a comprehensive analysis of the genetic diversity, function traits, and genome editing method of V. atypica remains elusive. In the present study, we conducted a systemically comparative analysis of the genetic datasets of nine V. atypica strains. The pan-genome of V. atypica consisted of 2,065 homologous clusters and exhibited an open pan-genome structure. A phylogenetic analysis of V. atypica with two different categories revealed that V. atypica OK5 was the most distant from the other eight V. atypica strains. A total of 43 orthologous genes were identified as CAZyme genes and grouped into 23 CAZyme families. The CAZyme components derived from accessory clusters contributed to the differences in the ability of the nine V. atypica strains to utilize carbohydrates. An integrated analysis of the metabolic pathways of V. atypica suggested that V. atypica strains harbored vancomycin resistance and were involved in several biosynthesis pathways of secondary metabolites. The V. atypica strains harbored four main Cas proteins, namely, CAS-Type IIIA, CAS-Type IIA, CAS-Type IIC, and CAS-Type IIID. This pilot study provides an in-depth understanding of and a fundamental knowledge about the biology of V. atypica that allow the possibility to increase the biotechnological potential of this bacterium. |
format | Online Article Text |
id | pubmed-7324755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73247552020-07-10 Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications Han, Maozhen Liu, Gang Chen, Yajun Wang, Dong Zhang, Yan Front Microbiol Microbiology Veillonella atypica is a bacterium that is present in the gut and the oral cavity of mammals and plays diverse roles in different niches. A recent study demonstrated that Veillonella is highly associated with marathon running and approved that V. atypica gavage improves treadmill run time in mice, revealing that V. atypica has a high biotechnological potential in improving athlete performance. However, a comprehensive analysis of the genetic diversity, function traits, and genome editing method of V. atypica remains elusive. In the present study, we conducted a systemically comparative analysis of the genetic datasets of nine V. atypica strains. The pan-genome of V. atypica consisted of 2,065 homologous clusters and exhibited an open pan-genome structure. A phylogenetic analysis of V. atypica with two different categories revealed that V. atypica OK5 was the most distant from the other eight V. atypica strains. A total of 43 orthologous genes were identified as CAZyme genes and grouped into 23 CAZyme families. The CAZyme components derived from accessory clusters contributed to the differences in the ability of the nine V. atypica strains to utilize carbohydrates. An integrated analysis of the metabolic pathways of V. atypica suggested that V. atypica strains harbored vancomycin resistance and were involved in several biosynthesis pathways of secondary metabolites. The V. atypica strains harbored four main Cas proteins, namely, CAS-Type IIIA, CAS-Type IIA, CAS-Type IIC, and CAS-Type IIID. This pilot study provides an in-depth understanding of and a fundamental knowledge about the biology of V. atypica that allow the possibility to increase the biotechnological potential of this bacterium. Frontiers Media S.A. 2020-06-23 /pmc/articles/PMC7324755/ /pubmed/32655519 http://dx.doi.org/10.3389/fmicb.2020.01219 Text en Copyright © 2020 Han, Liu, Chen, Wang and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Han, Maozhen Liu, Gang Chen, Yajun Wang, Dong Zhang, Yan Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications |
title | Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications |
title_full | Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications |
title_fullStr | Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications |
title_full_unstemmed | Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications |
title_short | Comparative Genomics Uncovers the Genetic Diversity and Characters of Veillonella atypica and Provides Insights Into Its Potential Applications |
title_sort | comparative genomics uncovers the genetic diversity and characters of veillonella atypica and provides insights into its potential applications |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324755/ https://www.ncbi.nlm.nih.gov/pubmed/32655519 http://dx.doi.org/10.3389/fmicb.2020.01219 |
work_keys_str_mv | AT hanmaozhen comparativegenomicsuncoversthegeneticdiversityandcharactersofveillonellaatypicaandprovidesinsightsintoitspotentialapplications AT liugang comparativegenomicsuncoversthegeneticdiversityandcharactersofveillonellaatypicaandprovidesinsightsintoitspotentialapplications AT chenyajun comparativegenomicsuncoversthegeneticdiversityandcharactersofveillonellaatypicaandprovidesinsightsintoitspotentialapplications AT wangdong comparativegenomicsuncoversthegeneticdiversityandcharactersofveillonellaatypicaandprovidesinsightsintoitspotentialapplications AT zhangyan comparativegenomicsuncoversthegeneticdiversityandcharactersofveillonellaatypicaandprovidesinsightsintoitspotentialapplications |