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Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013

Although intensive vaccination programs have been implemented, Newcastle disease (ND) outbreaks, accompanied by severe economic losses, are still reported in Egypt. The genetic characterization of ND virus (NDV) strains isolated from ND-vaccinated chicken flocks provides essential information for im...

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Autores principales: TRAN, Giang Thi Huong, SULTAN, Serageldeen, OSMAN, Nabila, HASSAN, Mohamed Ismail, VAN DONG, Hieu, DAO, Tung Duy, OMATSU, Tsutomu, KATAYAMA, Yukie, MIZUTANI, Tetsuya, TAKEDA, Yohei, OGAWA, Haruko, IMAI, Kunitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324829/
https://www.ncbi.nlm.nih.gov/pubmed/32307343
http://dx.doi.org/10.1292/jvms.19-0623
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author TRAN, Giang Thi Huong
SULTAN, Serageldeen
OSMAN, Nabila
HASSAN, Mohamed Ismail
VAN DONG, Hieu
DAO, Tung Duy
OMATSU, Tsutomu
KATAYAMA, Yukie
MIZUTANI, Tetsuya
TAKEDA, Yohei
OGAWA, Haruko
IMAI, Kunitoshi
author_facet TRAN, Giang Thi Huong
SULTAN, Serageldeen
OSMAN, Nabila
HASSAN, Mohamed Ismail
VAN DONG, Hieu
DAO, Tung Duy
OMATSU, Tsutomu
KATAYAMA, Yukie
MIZUTANI, Tetsuya
TAKEDA, Yohei
OGAWA, Haruko
IMAI, Kunitoshi
author_sort TRAN, Giang Thi Huong
collection PubMed
description Although intensive vaccination programs have been implemented, Newcastle disease (ND) outbreaks, accompanied by severe economic losses, are still reported in Egypt. The genetic characterization of ND virus (NDV) strains isolated from ND-vaccinated chicken flocks provides essential information for improving ND control strategies. Therefore, here, 38 NDV strains were isolated and identified from outbreaks among vaccinated flocks of broiler chickens located in the provinces of Qena, Luxor, and Aswan of Upper Egypt during 2011–2013. The investigated broiler chicken flocks (aged 28 to 40 days) had high mortality rates of up to 80%. All NDV isolates were genetically analyzed using next-generation DNA sequencing. From these isolates, 10 representative NDV strains were selected for further genetic analyses. Phylogenetic analysis of full-length coding genes revealed that the Egyptian NDV isolates belonged to a single sub-genotype, VII.1.1. These isolates were phylogenetically distant from the vaccine strains, including La Sota or Clone 30 (genotype II), which have been commonly used to vaccinate chicken flocks. Amino acid substitution K78R was observed in the neutralizing epitopes of the F proteins; whereas several mutations were found in the neutralizing epitopes of the hemagglutinin-neuraminidase proteins, notably, E347K. Overall, our results suggested that the occurrence of neutralizing epitope variants may be one of potential reasons for ND outbreaks. Further studies are needed to determine the protective effect of current vaccines against circulating virulent NDV strains.
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spelling pubmed-73248292020-07-02 Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013 TRAN, Giang Thi Huong SULTAN, Serageldeen OSMAN, Nabila HASSAN, Mohamed Ismail VAN DONG, Hieu DAO, Tung Duy OMATSU, Tsutomu KATAYAMA, Yukie MIZUTANI, Tetsuya TAKEDA, Yohei OGAWA, Haruko IMAI, Kunitoshi J Vet Med Sci Virology Although intensive vaccination programs have been implemented, Newcastle disease (ND) outbreaks, accompanied by severe economic losses, are still reported in Egypt. The genetic characterization of ND virus (NDV) strains isolated from ND-vaccinated chicken flocks provides essential information for improving ND control strategies. Therefore, here, 38 NDV strains were isolated and identified from outbreaks among vaccinated flocks of broiler chickens located in the provinces of Qena, Luxor, and Aswan of Upper Egypt during 2011–2013. The investigated broiler chicken flocks (aged 28 to 40 days) had high mortality rates of up to 80%. All NDV isolates were genetically analyzed using next-generation DNA sequencing. From these isolates, 10 representative NDV strains were selected for further genetic analyses. Phylogenetic analysis of full-length coding genes revealed that the Egyptian NDV isolates belonged to a single sub-genotype, VII.1.1. These isolates were phylogenetically distant from the vaccine strains, including La Sota or Clone 30 (genotype II), which have been commonly used to vaccinate chicken flocks. Amino acid substitution K78R was observed in the neutralizing epitopes of the F proteins; whereas several mutations were found in the neutralizing epitopes of the hemagglutinin-neuraminidase proteins, notably, E347K. Overall, our results suggested that the occurrence of neutralizing epitope variants may be one of potential reasons for ND outbreaks. Further studies are needed to determine the protective effect of current vaccines against circulating virulent NDV strains. The Japanese Society of Veterinary Science 2020-04-20 2020-06 /pmc/articles/PMC7324829/ /pubmed/32307343 http://dx.doi.org/10.1292/jvms.19-0623 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
TRAN, Giang Thi Huong
SULTAN, Serageldeen
OSMAN, Nabila
HASSAN, Mohamed Ismail
VAN DONG, Hieu
DAO, Tung Duy
OMATSU, Tsutomu
KATAYAMA, Yukie
MIZUTANI, Tetsuya
TAKEDA, Yohei
OGAWA, Haruko
IMAI, Kunitoshi
Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013
title Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013
title_full Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013
title_fullStr Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013
title_full_unstemmed Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013
title_short Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011–2013
title_sort molecular characterization of full genome sequences of newcastle disease viruses circulating among vaccinated chickens in egypt during 2011–2013
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324829/
https://www.ncbi.nlm.nih.gov/pubmed/32307343
http://dx.doi.org/10.1292/jvms.19-0623
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