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The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis

BTB-TAZ (BT)-domain proteins regulate plant development and pathogen defense. However, their roles in resistance to abiotic stresses remain largely unknown. In this study, we found that the sweetpotato BT protein-encoding gene IbBT4 significantly enhanced the drought tolerance of Arabidopsis. IbBT4...

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Autores principales: Zhou, Yuanyuan, Zhai, Hong, He, Shaozhen, Zhu, Hong, Gao, Shaopei, Xing, Shihan, Wei, Zihao, Zhao, Ning, Liu, Qingchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324939/
https://www.ncbi.nlm.nih.gov/pubmed/32655604
http://dx.doi.org/10.3389/fpls.2020.00877
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author Zhou, Yuanyuan
Zhai, Hong
He, Shaozhen
Zhu, Hong
Gao, Shaopei
Xing, Shihan
Wei, Zihao
Zhao, Ning
Liu, Qingchang
author_facet Zhou, Yuanyuan
Zhai, Hong
He, Shaozhen
Zhu, Hong
Gao, Shaopei
Xing, Shihan
Wei, Zihao
Zhao, Ning
Liu, Qingchang
author_sort Zhou, Yuanyuan
collection PubMed
description BTB-TAZ (BT)-domain proteins regulate plant development and pathogen defense. However, their roles in resistance to abiotic stresses remain largely unknown. In this study, we found that the sweetpotato BT protein-encoding gene IbBT4 significantly enhanced the drought tolerance of Arabidopsis. IbBT4 expression was induced by PEG6000, H(2)O(2) and brassinosteroids (BRs). The IbBT4-overexpressing Arabidopsis seeds presented higher germination rates and longer roots in comparison with those of WT under 200 mM mannitol stress. Under drought stress the transgenic Arabidopsis plants exhibited significantly increased survival rates and BR and proline contents and decreased water loss rates, MDA content and reactive oxygen species (ROS) levels. IbBT4 overexpression upregulated the BR signaling pathway and proline biosynthesis genes and activated the ROS-scavenging system under drought stress. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays revealed that the IbBT4 protein interacts with BR-ENHANCED EXPRESSION 2 (BEE2). Taken together, these results indicate that the IbBT4 gene provides drought tolerance by enhancing both the BR signaling pathway and proline biosynthesis and further activating the ROS-scavenging system in transgenic Arabidopsis.
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spelling pubmed-73249392020-07-10 The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis Zhou, Yuanyuan Zhai, Hong He, Shaozhen Zhu, Hong Gao, Shaopei Xing, Shihan Wei, Zihao Zhao, Ning Liu, Qingchang Front Plant Sci Plant Science BTB-TAZ (BT)-domain proteins regulate plant development and pathogen defense. However, their roles in resistance to abiotic stresses remain largely unknown. In this study, we found that the sweetpotato BT protein-encoding gene IbBT4 significantly enhanced the drought tolerance of Arabidopsis. IbBT4 expression was induced by PEG6000, H(2)O(2) and brassinosteroids (BRs). The IbBT4-overexpressing Arabidopsis seeds presented higher germination rates and longer roots in comparison with those of WT under 200 mM mannitol stress. Under drought stress the transgenic Arabidopsis plants exhibited significantly increased survival rates and BR and proline contents and decreased water loss rates, MDA content and reactive oxygen species (ROS) levels. IbBT4 overexpression upregulated the BR signaling pathway and proline biosynthesis genes and activated the ROS-scavenging system under drought stress. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays revealed that the IbBT4 protein interacts with BR-ENHANCED EXPRESSION 2 (BEE2). Taken together, these results indicate that the IbBT4 gene provides drought tolerance by enhancing both the BR signaling pathway and proline biosynthesis and further activating the ROS-scavenging system in transgenic Arabidopsis. Frontiers Media S.A. 2020-06-23 /pmc/articles/PMC7324939/ /pubmed/32655604 http://dx.doi.org/10.3389/fpls.2020.00877 Text en Copyright © 2020 Zhou, Zhai, He, Zhu, Gao, Xing, Wei, Zhao and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhou, Yuanyuan
Zhai, Hong
He, Shaozhen
Zhu, Hong
Gao, Shaopei
Xing, Shihan
Wei, Zihao
Zhao, Ning
Liu, Qingchang
The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis
title The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis
title_full The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis
title_fullStr The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis
title_full_unstemmed The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis
title_short The Sweetpotato BTB-TAZ Protein Gene, IbBT4, Enhances Drought Tolerance in Transgenic Arabidopsis
title_sort sweetpotato btb-taz protein gene, ibbt4, enhances drought tolerance in transgenic arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324939/
https://www.ncbi.nlm.nih.gov/pubmed/32655604
http://dx.doi.org/10.3389/fpls.2020.00877
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