Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation

[Image: see text] Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins an...

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Autores principales: Walker, Matthew, Will, Iain, Pratt, Andrew, Chechik, Victor, Genever, Paul, Ungar, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325428/
https://www.ncbi.nlm.nih.gov/pubmed/32626842
http://dx.doi.org/10.1021/acsanm.0c01167
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author Walker, Matthew
Will, Iain
Pratt, Andrew
Chechik, Victor
Genever, Paul
Ungar, Daniel
author_facet Walker, Matthew
Will, Iain
Pratt, Andrew
Chechik, Victor
Genever, Paul
Ungar, Daniel
author_sort Walker, Matthew
collection PubMed
description [Image: see text] Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below the polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically triggered release, upon which it could increase mesenchymal stem cell proliferation. Once the polymer transition will be chemically adjusted above body temperature, this system could be used for targeted cell stimulation in model animals and humans.
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spelling pubmed-73254282020-07-01 Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation Walker, Matthew Will, Iain Pratt, Andrew Chechik, Victor Genever, Paul Ungar, Daniel ACS Appl Nano Mater [Image: see text] Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below the polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically triggered release, upon which it could increase mesenchymal stem cell proliferation. Once the polymer transition will be chemically adjusted above body temperature, this system could be used for targeted cell stimulation in model animals and humans. American Chemical Society 2020-05-15 2020-06-26 /pmc/articles/PMC7325428/ /pubmed/32626842 http://dx.doi.org/10.1021/acsanm.0c01167 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Walker, Matthew
Will, Iain
Pratt, Andrew
Chechik, Victor
Genever, Paul
Ungar, Daniel
Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation
title Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation
title_full Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation
title_fullStr Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation
title_full_unstemmed Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation
title_short Magnetically Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem-Cell Proliferation
title_sort magnetically triggered release of entrapped bioactive proteins from thermally responsive polymer-coated iron oxide nanoparticles for stem-cell proliferation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325428/
https://www.ncbi.nlm.nih.gov/pubmed/32626842
http://dx.doi.org/10.1021/acsanm.0c01167
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