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Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity
Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, prot...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325951/ https://www.ncbi.nlm.nih.gov/pubmed/32655520 http://dx.doi.org/10.3389/fmicb.2020.01231 |
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author | Leastro, Mikhail Oliveira Castro, Deibis Yorlenis Ortega Freitas-Astúa, Juliana Kitajima, Elliot Watanabe Pallás, Vicente Sánchez-Navarro, Jesús Ángel |
author_facet | Leastro, Mikhail Oliveira Castro, Deibis Yorlenis Ortega Freitas-Astúa, Juliana Kitajima, Elliot Watanabe Pallás, Vicente Sánchez-Navarro, Jesús Ángel |
author_sort | Leastro, Mikhail Oliveira |
collection | PubMed |
description | Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. |
format | Online Article Text |
id | pubmed-7325951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73259512020-07-09 Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity Leastro, Mikhail Oliveira Castro, Deibis Yorlenis Ortega Freitas-Astúa, Juliana Kitajima, Elliot Watanabe Pallás, Vicente Sánchez-Navarro, Jesús Ángel Front Microbiol Microbiology Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. Frontiers Media S.A. 2020-06-09 /pmc/articles/PMC7325951/ /pubmed/32655520 http://dx.doi.org/10.3389/fmicb.2020.01231 Text en Copyright © 2020 Leastro, Castro, Freitas-Astúa, Kitajima, Pallás and Sánchez-Navarro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Leastro, Mikhail Oliveira Castro, Deibis Yorlenis Ortega Freitas-Astúa, Juliana Kitajima, Elliot Watanabe Pallás, Vicente Sánchez-Navarro, Jesús Ángel Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity |
title | Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity |
title_full | Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity |
title_fullStr | Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity |
title_full_unstemmed | Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity |
title_short | Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity |
title_sort | citrus leprosis virus c encodes three proteins with gene silencing suppression activity |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325951/ https://www.ncbi.nlm.nih.gov/pubmed/32655520 http://dx.doi.org/10.3389/fmicb.2020.01231 |
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