Cargando…

Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins

Ribosomal proteins (RPs) are essential components that translate genetic information from mRNA templates into proteins. Their expressional dysregulation adversely affects the survival and growth of human cells. Nevertheless, little is known about the nucleotide sequences regulating the expression of...

Descripción completa

Detalles Bibliográficos
Autores principales: Ryu, Jihye, Lee, Chaeyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326009/
https://www.ncbi.nlm.nih.gov/pubmed/32655613
http://dx.doi.org/10.3389/fgene.2020.00501
_version_ 1783552254768316416
author Ryu, Jihye
Lee, Chaeyoung
author_facet Ryu, Jihye
Lee, Chaeyoung
author_sort Ryu, Jihye
collection PubMed
description Ribosomal proteins (RPs) are essential components that translate genetic information from mRNA templates into proteins. Their expressional dysregulation adversely affects the survival and growth of human cells. Nevertheless, little is known about the nucleotide sequences regulating the expression of RPs. Genome-wide associations for expression level of 70 RP genes were conducted across expression stages. Eighteen expression regulatory quantitative trait loci (erQTLs) were identified for protein abundances of 21 RPs (P < 1 × 10(–5)), but not for their mRNA expression and ribosome occupancy (P > 1 × 10(–5)). These erQTLs for protein abundance (pQTLs) were all trans-acting. Three of the pQTLs were associated with the expression of long noncoding RNAs (lncRNAs). Target genes of these lncRNAs may produce ribosomal components or may control the metabolic cues for ribosome synthesis. mRNAs of the RP genes extensively interact with miRNAs. The protein-specific erQTLs may become engendered by intensive miRNA controls at the translational stage, which in turn can produce RPs efficient in handling instantaneous cell requirements. This study suggests that the expression levels of RPs may be greatly influenced by trans-acting regulation, presumably via interference of miRNAs and target genes of lncRNAs. Further studies are warranted to examine the molecular functions of pQTLs presented in this study and to understand the underlying regulatory mechanisms of gene expression of RPs.
format Online
Article
Text
id pubmed-7326009
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-73260092020-07-09 Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins Ryu, Jihye Lee, Chaeyoung Front Genet Genetics Ribosomal proteins (RPs) are essential components that translate genetic information from mRNA templates into proteins. Their expressional dysregulation adversely affects the survival and growth of human cells. Nevertheless, little is known about the nucleotide sequences regulating the expression of RPs. Genome-wide associations for expression level of 70 RP genes were conducted across expression stages. Eighteen expression regulatory quantitative trait loci (erQTLs) were identified for protein abundances of 21 RPs (P < 1 × 10(–5)), but not for their mRNA expression and ribosome occupancy (P > 1 × 10(–5)). These erQTLs for protein abundance (pQTLs) were all trans-acting. Three of the pQTLs were associated with the expression of long noncoding RNAs (lncRNAs). Target genes of these lncRNAs may produce ribosomal components or may control the metabolic cues for ribosome synthesis. mRNAs of the RP genes extensively interact with miRNAs. The protein-specific erQTLs may become engendered by intensive miRNA controls at the translational stage, which in turn can produce RPs efficient in handling instantaneous cell requirements. This study suggests that the expression levels of RPs may be greatly influenced by trans-acting regulation, presumably via interference of miRNAs and target genes of lncRNAs. Further studies are warranted to examine the molecular functions of pQTLs presented in this study and to understand the underlying regulatory mechanisms of gene expression of RPs. Frontiers Media S.A. 2020-06-05 /pmc/articles/PMC7326009/ /pubmed/32655613 http://dx.doi.org/10.3389/fgene.2020.00501 Text en Copyright © 2020 Ryu and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ryu, Jihye
Lee, Chaeyoung
Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins
title Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins
title_full Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins
title_fullStr Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins
title_full_unstemmed Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins
title_short Regulatory Nucleotide Sequence Signals for Expression of the Genes Encoding Ribosomal Proteins
title_sort regulatory nucleotide sequence signals for expression of the genes encoding ribosomal proteins
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326009/
https://www.ncbi.nlm.nih.gov/pubmed/32655613
http://dx.doi.org/10.3389/fgene.2020.00501
work_keys_str_mv AT ryujihye regulatorynucleotidesequencesignalsforexpressionofthegenesencodingribosomalproteins
AT leechaeyoung regulatorynucleotidesequencesignalsforexpressionofthegenesencodingribosomalproteins