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Larger active site in an ancestral hydroxynitrile lyase increases catalytically promiscuous esterase activity

Hydroxynitrile lyases (HNL's) belonging to the α/β-hydrolase-fold superfamily evolved from esterases approximately 100 million years ago. Reconstruction of an ancestral hydroxynitrile lyase in the α/β-hydrolase fold superfamily yielded a catalytically active hydroxynitrile lyase, HNL1. Several...

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Detalles Bibliográficos
Autores principales:	Jones, Bryan J., Evans, Robert L., Mylrea, Nathan J., Chaudhury, Debayan, Luo, Christine, Guan, Bo, Pierce, Colin T., Gordon, Wendy R., Wilmot, Carrie M., Kazlauskas, Romas J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326234/ https://www.ncbi.nlm.nih.gov/pubmed/32603354 http://dx.doi.org/10.1371/journal.pone.0235341

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