Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae

Since the end of 2019, the global COVID-19 outbreak has once again made coronaviruses a hot topic. Vaccines are hoped to be an effective way to stop the spread of the virus. However, there are no clinically approved vaccines available for coronavirus infections. Reverse genetics technology can reali...

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Detalles Bibliográficos
Autores principales: Ma, Zhiqian, Li, Zhiwei, Dong, Linfang, Yang, Ting, Xiao, Shuqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326460/
https://www.ncbi.nlm.nih.gov/pubmed/32711735
http://dx.doi.org/10.1016/bs.aivir.2020.06.003
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author Ma, Zhiqian
Li, Zhiwei
Dong, Linfang
Yang, Ting
Xiao, Shuqi
author_facet Ma, Zhiqian
Li, Zhiwei
Dong, Linfang
Yang, Ting
Xiao, Shuqi
author_sort Ma, Zhiqian
collection PubMed
description Since the end of 2019, the global COVID-19 outbreak has once again made coronaviruses a hot topic. Vaccines are hoped to be an effective way to stop the spread of the virus. However, there are no clinically approved vaccines available for coronavirus infections. Reverse genetics technology can realize the operation of RNA virus genomes at the DNA level and provide new ideas and strategies for the development of new vaccines. In this review, we systematically describe the role of reverse genetics technology in studying the effects of coronavirus proteins on viral virulence and innate immunity, cell and tissue tropism and antiviral drug screening. An efficient reverse genetics platform is useful for obtaining the ideal attenuated strain to prepare an attenuated live vaccine.
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spelling pubmed-73264602020-07-01 Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae Ma, Zhiqian Li, Zhiwei Dong, Linfang Yang, Ting Xiao, Shuqi Adv Virus Res Article Since the end of 2019, the global COVID-19 outbreak has once again made coronaviruses a hot topic. Vaccines are hoped to be an effective way to stop the spread of the virus. However, there are no clinically approved vaccines available for coronavirus infections. Reverse genetics technology can realize the operation of RNA virus genomes at the DNA level and provide new ideas and strategies for the development of new vaccines. In this review, we systematically describe the role of reverse genetics technology in studying the effects of coronavirus proteins on viral virulence and innate immunity, cell and tissue tropism and antiviral drug screening. An efficient reverse genetics platform is useful for obtaining the ideal attenuated strain to prepare an attenuated live vaccine. Elsevier Inc. 2020 2020-06-30 /pmc/articles/PMC7326460/ /pubmed/32711735 http://dx.doi.org/10.1016/bs.aivir.2020.06.003 Text en Copyright © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ma, Zhiqian
Li, Zhiwei
Dong, Linfang
Yang, Ting
Xiao, Shuqi
Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae
title Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae
title_full Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae
title_fullStr Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae
title_full_unstemmed Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae
title_short Reverse genetic systems: Rational design of coronavirus live attenuated vaccines with immune sequelae
title_sort reverse genetic systems: rational design of coronavirus live attenuated vaccines with immune sequelae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326460/
https://www.ncbi.nlm.nih.gov/pubmed/32711735
http://dx.doi.org/10.1016/bs.aivir.2020.06.003
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