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New Twists in Detecting mRNA Modification Dynamics

Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modif...

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Autores principales: Anreiter, Ina, Mir, Quoseena, Simpson, Jared T., Janga, Sarath C., Soller, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326690/
https://www.ncbi.nlm.nih.gov/pubmed/32620324
http://dx.doi.org/10.1016/j.tibtech.2020.06.002
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author Anreiter, Ina
Mir, Quoseena
Simpson, Jared T.
Janga, Sarath C.
Soller, Matthias
author_facet Anreiter, Ina
Mir, Quoseena
Simpson, Jared T.
Janga, Sarath C.
Soller, Matthias
author_sort Anreiter, Ina
collection PubMed
description Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.
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spelling pubmed-73266902020-07-01 New Twists in Detecting mRNA Modification Dynamics Anreiter, Ina Mir, Quoseena Simpson, Jared T. Janga, Sarath C. Soller, Matthias Trends Biotechnol Review Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology. Elsevier Ltd. 2021-01 2020-07-01 /pmc/articles/PMC7326690/ /pubmed/32620324 http://dx.doi.org/10.1016/j.tibtech.2020.06.002 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Review
Anreiter, Ina
Mir, Quoseena
Simpson, Jared T.
Janga, Sarath C.
Soller, Matthias
New Twists in Detecting mRNA Modification Dynamics
title New Twists in Detecting mRNA Modification Dynamics
title_full New Twists in Detecting mRNA Modification Dynamics
title_fullStr New Twists in Detecting mRNA Modification Dynamics
title_full_unstemmed New Twists in Detecting mRNA Modification Dynamics
title_short New Twists in Detecting mRNA Modification Dynamics
title_sort new twists in detecting mrna modification dynamics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326690/
https://www.ncbi.nlm.nih.gov/pubmed/32620324
http://dx.doi.org/10.1016/j.tibtech.2020.06.002
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