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Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System

Planktonic heterotrophic diazotrophs (N(2)-fixers) are widely distributed in marine and freshwater systems, yet limited information is available on their activity, especially in environments with adverse conditions for diazotrophy (e.g., N-rich and oxygenated). Here, we followed the localization and...

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Autores principales: Geisler, Eyal, Bogler, Anne, Bar-Zeev, Edo, Rahav, Eyal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326945/
https://www.ncbi.nlm.nih.gov/pubmed/32670236
http://dx.doi.org/10.3389/fmicb.2020.01370
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author Geisler, Eyal
Bogler, Anne
Bar-Zeev, Edo
Rahav, Eyal
author_facet Geisler, Eyal
Bogler, Anne
Bar-Zeev, Edo
Rahav, Eyal
author_sort Geisler, Eyal
collection PubMed
description Planktonic heterotrophic diazotrophs (N(2)-fixers) are widely distributed in marine and freshwater systems, yet limited information is available on their activity, especially in environments with adverse conditions for diazotrophy (e.g., N-rich and oxygenated). Here, we followed the localization and activity of heterotrophic diazotrophs in the hyper-eutrophic N-rich Qishon River—an environment previously considered to be unfavorable for diazotrophy. Our results indicate high heterotrophic N(2) fixation rates (up to 6.9 nmol N L(–1) d(–1)), which were approximately three fold higher at an upstream location (freshwater) compared to an estuary (brackish) site. Further, active heterotrophic diazotrophs were capture associated with free-floating aggregates by a newly developed immunolocalization approach. These findings provide new insights on the activity of heterotrophic diazotrophs on aggregates in environments previously considered with adverse conditions for diazotrophy. Moreover, these new insights may be applicable to other aquatic regimes worldwide with similar N-rich/oxygenated conditions that should potentially inhibit N(2) fixation.
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spelling pubmed-73269452020-07-14 Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System Geisler, Eyal Bogler, Anne Bar-Zeev, Edo Rahav, Eyal Front Microbiol Microbiology Planktonic heterotrophic diazotrophs (N(2)-fixers) are widely distributed in marine and freshwater systems, yet limited information is available on their activity, especially in environments with adverse conditions for diazotrophy (e.g., N-rich and oxygenated). Here, we followed the localization and activity of heterotrophic diazotrophs in the hyper-eutrophic N-rich Qishon River—an environment previously considered to be unfavorable for diazotrophy. Our results indicate high heterotrophic N(2) fixation rates (up to 6.9 nmol N L(–1) d(–1)), which were approximately three fold higher at an upstream location (freshwater) compared to an estuary (brackish) site. Further, active heterotrophic diazotrophs were capture associated with free-floating aggregates by a newly developed immunolocalization approach. These findings provide new insights on the activity of heterotrophic diazotrophs on aggregates in environments previously considered with adverse conditions for diazotrophy. Moreover, these new insights may be applicable to other aquatic regimes worldwide with similar N-rich/oxygenated conditions that should potentially inhibit N(2) fixation. Frontiers Media S.A. 2020-06-24 /pmc/articles/PMC7326945/ /pubmed/32670236 http://dx.doi.org/10.3389/fmicb.2020.01370 Text en Copyright © 2020 Geisler, Bogler, Bar-Zeev and Rahav. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Geisler, Eyal
Bogler, Anne
Bar-Zeev, Edo
Rahav, Eyal
Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System
title Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System
title_full Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System
title_fullStr Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System
title_full_unstemmed Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System
title_short Heterotrophic Nitrogen Fixation at the Hyper-Eutrophic Qishon River and Estuary System
title_sort heterotrophic nitrogen fixation at the hyper-eutrophic qishon river and estuary system
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326945/
https://www.ncbi.nlm.nih.gov/pubmed/32670236
http://dx.doi.org/10.3389/fmicb.2020.01370
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