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Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach

Black Soldier Fly (BSF) meal is considered as an alternative, emerging and sustainable ingredient for aquafeed production. However, results on fish physiological responses are still fragmentary and often controversial, while no studies are available on fish behavior in response to these new diets. T...

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Autores principales: Zarantoniello, Matteo, Randazzo, Basilio, Gioacchini, Giorgia, Truzzi, Cristina, Giorgini, Elisabetta, Riolo, Paola, Gioia, Giorgia, Bertolucci, Cristiano, Osimani, Andrea, Cardinaletti, Gloriana, Lucon-Xiccato, Tyrone, Milanović, Vesna, Annibaldi, Anna, Tulli, Francesca, Notarstefano, Valentina, Ruschioni, Sara, Clementi, Francesca, Olivotto, Ike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326965/
https://www.ncbi.nlm.nih.gov/pubmed/32606335
http://dx.doi.org/10.1038/s41598-020-67740-w
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author Zarantoniello, Matteo
Randazzo, Basilio
Gioacchini, Giorgia
Truzzi, Cristina
Giorgini, Elisabetta
Riolo, Paola
Gioia, Giorgia
Bertolucci, Cristiano
Osimani, Andrea
Cardinaletti, Gloriana
Lucon-Xiccato, Tyrone
Milanović, Vesna
Annibaldi, Anna
Tulli, Francesca
Notarstefano, Valentina
Ruschioni, Sara
Clementi, Francesca
Olivotto, Ike
author_facet Zarantoniello, Matteo
Randazzo, Basilio
Gioacchini, Giorgia
Truzzi, Cristina
Giorgini, Elisabetta
Riolo, Paola
Gioia, Giorgia
Bertolucci, Cristiano
Osimani, Andrea
Cardinaletti, Gloriana
Lucon-Xiccato, Tyrone
Milanović, Vesna
Annibaldi, Anna
Tulli, Francesca
Notarstefano, Valentina
Ruschioni, Sara
Clementi, Francesca
Olivotto, Ike
author_sort Zarantoniello, Matteo
collection PubMed
description Black Soldier Fly (BSF) meal is considered as an alternative, emerging and sustainable ingredient for aquafeed production. However, results on fish physiological responses are still fragmentary and often controversial, while no studies are available on fish behavior in response to these new diets. The present work represents the first comprehensive multidisciplinary study aimed to investigate zebrafish physiological and behavioural responses to BSF-based diets. Five experimental diets characterized by increasing inclusion levels (0, 25, 50, 75 and 100% respect to fish meal) of full fat BSF prepupae meal were tested during a 2-months feeding trial. Prepupae were cultured on coffee silverskin growth substrate enriched with a 10% Schizochytrium sp. to improve insects’ fatty acids profile. The responses of zebrafish were assayed through biometric, histological, gas chromatographic, microbiological, spectroscopic, molecular and behavioural analyses. Results evidenced that BSF-based diets affected fish fatty acid composition, while behavioural tests did not show differences among groups. Specifically, a 50% BSF inclusion level diet represented the best compromise between ingredient sustainability and proper fish growth and welfare. Fish fed with higher BSF inclusions (75 and 100%) showed hepatic steatosis, microbiota modification, higher lipid content, fatty acid modification and higher expression of immune response markers.
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spelling pubmed-73269652020-07-01 Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach Zarantoniello, Matteo Randazzo, Basilio Gioacchini, Giorgia Truzzi, Cristina Giorgini, Elisabetta Riolo, Paola Gioia, Giorgia Bertolucci, Cristiano Osimani, Andrea Cardinaletti, Gloriana Lucon-Xiccato, Tyrone Milanović, Vesna Annibaldi, Anna Tulli, Francesca Notarstefano, Valentina Ruschioni, Sara Clementi, Francesca Olivotto, Ike Sci Rep Article Black Soldier Fly (BSF) meal is considered as an alternative, emerging and sustainable ingredient for aquafeed production. However, results on fish physiological responses are still fragmentary and often controversial, while no studies are available on fish behavior in response to these new diets. The present work represents the first comprehensive multidisciplinary study aimed to investigate zebrafish physiological and behavioural responses to BSF-based diets. Five experimental diets characterized by increasing inclusion levels (0, 25, 50, 75 and 100% respect to fish meal) of full fat BSF prepupae meal were tested during a 2-months feeding trial. Prepupae were cultured on coffee silverskin growth substrate enriched with a 10% Schizochytrium sp. to improve insects’ fatty acids profile. The responses of zebrafish were assayed through biometric, histological, gas chromatographic, microbiological, spectroscopic, molecular and behavioural analyses. Results evidenced that BSF-based diets affected fish fatty acid composition, while behavioural tests did not show differences among groups. Specifically, a 50% BSF inclusion level diet represented the best compromise between ingredient sustainability and proper fish growth and welfare. Fish fed with higher BSF inclusions (75 and 100%) showed hepatic steatosis, microbiota modification, higher lipid content, fatty acid modification and higher expression of immune response markers. Nature Publishing Group UK 2020-06-30 /pmc/articles/PMC7326965/ /pubmed/32606335 http://dx.doi.org/10.1038/s41598-020-67740-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zarantoniello, Matteo
Randazzo, Basilio
Gioacchini, Giorgia
Truzzi, Cristina
Giorgini, Elisabetta
Riolo, Paola
Gioia, Giorgia
Bertolucci, Cristiano
Osimani, Andrea
Cardinaletti, Gloriana
Lucon-Xiccato, Tyrone
Milanović, Vesna
Annibaldi, Anna
Tulli, Francesca
Notarstefano, Valentina
Ruschioni, Sara
Clementi, Francesca
Olivotto, Ike
Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
title Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
title_full Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
title_fullStr Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
title_full_unstemmed Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
title_short Zebrafish (Danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
title_sort zebrafish (danio rerio) physiological and behavioural responses to insect-based diets: a multidisciplinary approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326965/
https://www.ncbi.nlm.nih.gov/pubmed/32606335
http://dx.doi.org/10.1038/s41598-020-67740-w
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