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Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum

Flavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about...

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Autores principales: Ren, Zhiyao, Ji, Xiaoyu, Jiao, Zhenbin, Luo, Yingyi, Zhang, Guo-Qiang, Tao, Shengchang, Lei, Zhouxi, Zhang, Jing, Wang, Yuchen, Liu, Zhong-Jian, Wei, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326982/
https://www.ncbi.nlm.nih.gov/pubmed/32637139
http://dx.doi.org/10.1038/s41438-020-0330-4
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author Ren, Zhiyao
Ji, Xiaoyu
Jiao, Zhenbin
Luo, Yingyi
Zhang, Guo-Qiang
Tao, Shengchang
Lei, Zhouxi
Zhang, Jing
Wang, Yuchen
Liu, Zhong-Jian
Wei, Gang
author_facet Ren, Zhiyao
Ji, Xiaoyu
Jiao, Zhenbin
Luo, Yingyi
Zhang, Guo-Qiang
Tao, Shengchang
Lei, Zhouxi
Zhang, Jing
Wang, Yuchen
Liu, Zhong-Jian
Wei, Gang
author_sort Ren, Zhiyao
collection PubMed
description Flavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about glycosyltransferase in the orchid Dendrobium catenatum (D. officinale). In this study, we isolated a novel C-glycosyltransferase (designated DcaCGT) from the orchid D. catenatum by identifying and analyzing 82 putative genes in the GT1 family. DcaCGT could specifically catalyze not only di-C-glycosylation but also O-glycosylation. Apart from the normal function of catalyzing 2-hydroxynaringenin and phloretin to the respective di-C-glycosides, DcaCGT also catalyzes apigenin to cosmosiin. Targeted metabolic profiling of the substrates (2-hydroxynaringenin, phloretin, and apigenin) and products (vitexin, isovitexin, vicenin-2, nothofagin, 3’,5’-di-C-glucosylphloretin, and cosmosiin) in different tissues showed that vicenin-2 was the most abundant product of this novel enzyme. Cosmosiin was detected in flowers and flower buds. We also established that DcaCGT functions expanded throughout the evolution of D. catenatum. Residual OGT activity may help D. catenatum resist drought stress. Our study illustrates the function, origin, and differentiation of DcaCGT and provides insights into glycosylation and molecular propagation processes, which can be used to improve the production of flavonoids by the cultivated medicinal plant D. catenatum.
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spelling pubmed-73269822020-07-06 Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum Ren, Zhiyao Ji, Xiaoyu Jiao, Zhenbin Luo, Yingyi Zhang, Guo-Qiang Tao, Shengchang Lei, Zhouxi Zhang, Jing Wang, Yuchen Liu, Zhong-Jian Wei, Gang Hortic Res Article Flavonoids, which are a diverse class of phytonutrients, are used by organisms to respond to nearly all abiotic stresses and are beneficial for human health. Glycosyltransferase, used during the last step of flavonoid biosynthesis, is important in flavonoid enrichment. However, little is known about glycosyltransferase in the orchid Dendrobium catenatum (D. officinale). In this study, we isolated a novel C-glycosyltransferase (designated DcaCGT) from the orchid D. catenatum by identifying and analyzing 82 putative genes in the GT1 family. DcaCGT could specifically catalyze not only di-C-glycosylation but also O-glycosylation. Apart from the normal function of catalyzing 2-hydroxynaringenin and phloretin to the respective di-C-glycosides, DcaCGT also catalyzes apigenin to cosmosiin. Targeted metabolic profiling of the substrates (2-hydroxynaringenin, phloretin, and apigenin) and products (vitexin, isovitexin, vicenin-2, nothofagin, 3’,5’-di-C-glucosylphloretin, and cosmosiin) in different tissues showed that vicenin-2 was the most abundant product of this novel enzyme. Cosmosiin was detected in flowers and flower buds. We also established that DcaCGT functions expanded throughout the evolution of D. catenatum. Residual OGT activity may help D. catenatum resist drought stress. Our study illustrates the function, origin, and differentiation of DcaCGT and provides insights into glycosylation and molecular propagation processes, which can be used to improve the production of flavonoids by the cultivated medicinal plant D. catenatum. Nature Publishing Group UK 2020-07-01 /pmc/articles/PMC7326982/ /pubmed/32637139 http://dx.doi.org/10.1038/s41438-020-0330-4 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ren, Zhiyao
Ji, Xiaoyu
Jiao, Zhenbin
Luo, Yingyi
Zhang, Guo-Qiang
Tao, Shengchang
Lei, Zhouxi
Zhang, Jing
Wang, Yuchen
Liu, Zhong-Jian
Wei, Gang
Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
title Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
title_full Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
title_fullStr Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
title_full_unstemmed Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
title_short Functional analysis of a novel C-glycosyltransferase in the orchid Dendrobium catenatum
title_sort functional analysis of a novel c-glycosyltransferase in the orchid dendrobium catenatum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7326982/
https://www.ncbi.nlm.nih.gov/pubmed/32637139
http://dx.doi.org/10.1038/s41438-020-0330-4
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