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MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5

Overuse of fungicides and fertilizers has resulted in copper (Cu) contamination of soils and toxic levels of Cu in apple fruits. To breed Cu-resistant apple (Malus domestica) cultivars, the underlying molecular mechanisms and key genes involved in Cu resistance must be identified. Here, we show that...

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Autores principales: Shi, Kun, Liu, Xuan, Zhu, Yunpeng, Bai, Yixue, Shan, Dongqian, Zheng, Xiaodong, Wang, Lin, Zhang, Haixia, Wang, Chanyu, Yan, Tianci, Zhou, Fangfang, Hu, Zehui, Sun, Yanzhao, Guo, Yan, Kong, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327004/
https://www.ncbi.nlm.nih.gov/pubmed/32637133
http://dx.doi.org/10.1038/s41438-020-0326-0
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author Shi, Kun
Liu, Xuan
Zhu, Yunpeng
Bai, Yixue
Shan, Dongqian
Zheng, Xiaodong
Wang, Lin
Zhang, Haixia
Wang, Chanyu
Yan, Tianci
Zhou, Fangfang
Hu, Zehui
Sun, Yanzhao
Guo, Yan
Kong, Jin
author_facet Shi, Kun
Liu, Xuan
Zhu, Yunpeng
Bai, Yixue
Shan, Dongqian
Zheng, Xiaodong
Wang, Lin
Zhang, Haixia
Wang, Chanyu
Yan, Tianci
Zhou, Fangfang
Hu, Zehui
Sun, Yanzhao
Guo, Yan
Kong, Jin
author_sort Shi, Kun
collection PubMed
description Overuse of fungicides and fertilizers has resulted in copper (Cu) contamination of soils and toxic levels of Cu in apple fruits. To breed Cu-resistant apple (Malus domestica) cultivars, the underlying molecular mechanisms and key genes involved in Cu resistance must be identified. Here, we show that MdWRKY11 increases Cu tolerance by directly promoting the transcription of MdHMA5. MdHMA5 is a Cu transporter that may function in the storage of excess Cu in root cell walls and stems for Cu tolerance in apple. The transcription factor MdWRKY11 is highly induced by excess Cu. MdWRKY11 overexpression in transgenic apple enhanced Cu tolerance and decreased Cu accumulation. Apple calli transformed with an MdWRKY11-RNAi construct exhibited the opposite phenotype. Both an in vivo chromatin immunoprecipitation assay and an in vitro electrophoretic mobility shift assay indicated that MdWRKY11 binds to the promoter of MdHMA5. Furthermore, MdWRKY11 promoted MdHMA5 expression in transgenic apple plants, as revealed by quantitative PCR. Moreover, inhibition of MdWRKY11 expression by RNA interference led to a significant decrease in MdHMA5 transcription. Thus, MdWRKY11 directly regulates MdHMA5 transcription. Our work resulted in the identification of a novel MdWRKY11-MdHMA5 pathway that mediates Cu resistance in apple.
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spelling pubmed-73270042020-07-06 MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5 Shi, Kun Liu, Xuan Zhu, Yunpeng Bai, Yixue Shan, Dongqian Zheng, Xiaodong Wang, Lin Zhang, Haixia Wang, Chanyu Yan, Tianci Zhou, Fangfang Hu, Zehui Sun, Yanzhao Guo, Yan Kong, Jin Hortic Res Article Overuse of fungicides and fertilizers has resulted in copper (Cu) contamination of soils and toxic levels of Cu in apple fruits. To breed Cu-resistant apple (Malus domestica) cultivars, the underlying molecular mechanisms and key genes involved in Cu resistance must be identified. Here, we show that MdWRKY11 increases Cu tolerance by directly promoting the transcription of MdHMA5. MdHMA5 is a Cu transporter that may function in the storage of excess Cu in root cell walls and stems for Cu tolerance in apple. The transcription factor MdWRKY11 is highly induced by excess Cu. MdWRKY11 overexpression in transgenic apple enhanced Cu tolerance and decreased Cu accumulation. Apple calli transformed with an MdWRKY11-RNAi construct exhibited the opposite phenotype. Both an in vivo chromatin immunoprecipitation assay and an in vitro electrophoretic mobility shift assay indicated that MdWRKY11 binds to the promoter of MdHMA5. Furthermore, MdWRKY11 promoted MdHMA5 expression in transgenic apple plants, as revealed by quantitative PCR. Moreover, inhibition of MdWRKY11 expression by RNA interference led to a significant decrease in MdHMA5 transcription. Thus, MdWRKY11 directly regulates MdHMA5 transcription. Our work resulted in the identification of a novel MdWRKY11-MdHMA5 pathway that mediates Cu resistance in apple. Nature Publishing Group UK 2020-07-01 /pmc/articles/PMC7327004/ /pubmed/32637133 http://dx.doi.org/10.1038/s41438-020-0326-0 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shi, Kun
Liu, Xuan
Zhu, Yunpeng
Bai, Yixue
Shan, Dongqian
Zheng, Xiaodong
Wang, Lin
Zhang, Haixia
Wang, Chanyu
Yan, Tianci
Zhou, Fangfang
Hu, Zehui
Sun, Yanzhao
Guo, Yan
Kong, Jin
MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5
title MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5
title_full MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5
title_fullStr MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5
title_full_unstemmed MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5
title_short MdWRKY11 improves copper tolerance by directly promoting the expression of the copper transporter gene MdHMA5
title_sort mdwrky11 improves copper tolerance by directly promoting the expression of the copper transporter gene mdhma5
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327004/
https://www.ncbi.nlm.nih.gov/pubmed/32637133
http://dx.doi.org/10.1038/s41438-020-0326-0
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