Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni

Chrysanthemum is frequently attacked by aphids, which greatly hinders the growth and ornamental value of this plant species. WRKY transcription factors play an important role in the response to biotic stresses such as pathogen and insect stresses. Here, chrysanthemum CmWRKY53 was cloned, and its exp...

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Autores principales: Zhang, Wanwan, Gao, Tianwei, Li, Peiling, Tian, Chang, Song, Aiping, Jiang, Jiafu, Guan, Zhiyong, Fang, Weimin, Chen, Fadi, Chen, Sumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327015/
https://www.ncbi.nlm.nih.gov/pubmed/32637137
http://dx.doi.org/10.1038/s41438-020-0334-0
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author Zhang, Wanwan
Gao, Tianwei
Li, Peiling
Tian, Chang
Song, Aiping
Jiang, Jiafu
Guan, Zhiyong
Fang, Weimin
Chen, Fadi
Chen, Sumei
author_facet Zhang, Wanwan
Gao, Tianwei
Li, Peiling
Tian, Chang
Song, Aiping
Jiang, Jiafu
Guan, Zhiyong
Fang, Weimin
Chen, Fadi
Chen, Sumei
author_sort Zhang, Wanwan
collection PubMed
description Chrysanthemum is frequently attacked by aphids, which greatly hinders the growth and ornamental value of this plant species. WRKY transcription factors play an important role in the response to biotic stresses such as pathogen and insect stresses. Here, chrysanthemum CmWRKY53 was cloned, and its expression was induced by aphid infestation. To verify the role of CmWRKY53 in resistance to aphids, CmWRKY53 transgenic chrysanthemum was generated. CmWRKY53 was found to mediate the susceptibility of chrysanthemum to aphids. The expression levels of secondary metabolite biosynthesis genes, such as peroxidase- and polyphenol oxidase-encoding genes, decreased in CmWRKY53-overexpressing (CmWRKY53-Oe) plants but dramatically increased in chimeric dominant repressor (CmWRKY53-SRDX) plants, suggesting that CmWRKY53 contributes to the susceptibility of chrysanthemum to aphids, possibly due to its role in the regulation of secondary metabolites.
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spelling pubmed-73270152020-07-06 Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni Zhang, Wanwan Gao, Tianwei Li, Peiling Tian, Chang Song, Aiping Jiang, Jiafu Guan, Zhiyong Fang, Weimin Chen, Fadi Chen, Sumei Hortic Res Article Chrysanthemum is frequently attacked by aphids, which greatly hinders the growth and ornamental value of this plant species. WRKY transcription factors play an important role in the response to biotic stresses such as pathogen and insect stresses. Here, chrysanthemum CmWRKY53 was cloned, and its expression was induced by aphid infestation. To verify the role of CmWRKY53 in resistance to aphids, CmWRKY53 transgenic chrysanthemum was generated. CmWRKY53 was found to mediate the susceptibility of chrysanthemum to aphids. The expression levels of secondary metabolite biosynthesis genes, such as peroxidase- and polyphenol oxidase-encoding genes, decreased in CmWRKY53-overexpressing (CmWRKY53-Oe) plants but dramatically increased in chimeric dominant repressor (CmWRKY53-SRDX) plants, suggesting that CmWRKY53 contributes to the susceptibility of chrysanthemum to aphids, possibly due to its role in the regulation of secondary metabolites. Nature Publishing Group UK 2020-07-01 /pmc/articles/PMC7327015/ /pubmed/32637137 http://dx.doi.org/10.1038/s41438-020-0334-0 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhang, Wanwan
Gao, Tianwei
Li, Peiling
Tian, Chang
Song, Aiping
Jiang, Jiafu
Guan, Zhiyong
Fang, Weimin
Chen, Fadi
Chen, Sumei
Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni
title Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni
title_full Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni
title_fullStr Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni
title_full_unstemmed Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni
title_short Chrysanthemum CmWRKY53 negatively regulates the resistance of chrysanthemum to the aphid Macrosiphoniella sanborni
title_sort chrysanthemum cmwrky53 negatively regulates the resistance of chrysanthemum to the aphid macrosiphoniella sanborni
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327015/
https://www.ncbi.nlm.nih.gov/pubmed/32637137
http://dx.doi.org/10.1038/s41438-020-0334-0
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