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Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy

BACKGROUND: Medications to reduce oxidative stress are preventing cellular damage associated with hyperlipidemia. In this regard, statins (e.g., atorvastatin) act primarily by decrease in low-density lipoprotein-c but, in the last decade, hepatotoxicity, associated with liver injuries in the next mo...

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Autores principales: Mohamed, Erieg A., Bordean, Despina M., Radulov, Isidora, Moruzi, Răzvan F., Hulea, Călin I., Orășan, Sergiu A., Dumitrescu, Eugenia, Muselin, Florin, Herman, Hildegard, Brezovan, Diana, Hermenean, Anca, Cristina, Romeo T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327606/
https://www.ncbi.nlm.nih.gov/pubmed/32655657
http://dx.doi.org/10.1155/2020/1736803
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author Mohamed, Erieg A.
Bordean, Despina M.
Radulov, Isidora
Moruzi, Răzvan F.
Hulea, Călin I.
Orășan, Sergiu A.
Dumitrescu, Eugenia
Muselin, Florin
Herman, Hildegard
Brezovan, Diana
Hermenean, Anca
Cristina, Romeo T.
author_facet Mohamed, Erieg A.
Bordean, Despina M.
Radulov, Isidora
Moruzi, Răzvan F.
Hulea, Călin I.
Orășan, Sergiu A.
Dumitrescu, Eugenia
Muselin, Florin
Herman, Hildegard
Brezovan, Diana
Hermenean, Anca
Cristina, Romeo T.
author_sort Mohamed, Erieg A.
collection PubMed
description BACKGROUND: Medications to reduce oxidative stress are preventing cellular damage associated with hyperlipidemia. In this regard, statins (e.g., atorvastatin) act primarily by decrease in low-density lipoprotein-c but, in the last decade, hepatotoxicity, associated with liver injuries in the next months after treatments' initiation, was reported. In this case, associated phytotherapy can be a solution. PURPOSE: To investigate the antioxidant potential and response to free radicals, in the case of hyperlipidemic rats treated with atorvastatin. Sea buckthorn (Hippophae rhamnoides) and a grape extract (antioxivita) efficiency in the oxidative stress were investigated, also being ascertained the rats' organs cytoarchitecture. METHODS: Eighty-four hyperlipidemic Wistar rats were divided into seven groups and orally treated as follows: ATS, atorvastatin (20 mg/kg·bw); ATS + Hr, atorvastatin + H. rhamnoides; ATS + Aox, atorvastatin + grape extract; Hr, H. rhamnoides; and Aox, grape extract (both as 100 mg/kg·bw). HFD and Control received high fat diet and normal fodder only. After two and six months, respectively, rats were euthanized and the heart, liver, and kidneys were gathered. The tissue samples were prepared by homogenization of 0.5 g tissue, in ethanol, kept for 48 hours at 4°C–10°C and then filtered, in order to assess organs' cytoarchitecture and the TAC's values (by using cupric ion reducing antioxidant capacity (CUPRAC) assay). The test tubes were incubated, at room temperature, for 30 minutes, and then analyzed using a spectrophotometer at 450–650 nm. RESULTS: The statistics (ANOVA) revealed that sea buckthorn diminished notably (p < 0.001) the oxidative stress in the heart, liver, and kidney. After six months, the TAC's reduced levels for the heart were significant (p < 0.001) in ATS + Aox. In the case of histology, the liver's cytoarchitecture in ATS revealed abnormal cytoarchitecture. In ATS + Hr, ATS + Aox, Hr, and Aox, cell regeneration improved in different stages, especially for ATS + Hr and ATS + Aox, in comparison with HFD, which exhibited fat degeneration. Kidney's cytoarchitecture revealed cellular healing, especially in ATS + Hr and ATS + Aox.
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spelling pubmed-73276062020-07-11 Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy Mohamed, Erieg A. Bordean, Despina M. Radulov, Isidora Moruzi, Răzvan F. Hulea, Călin I. Orășan, Sergiu A. Dumitrescu, Eugenia Muselin, Florin Herman, Hildegard Brezovan, Diana Hermenean, Anca Cristina, Romeo T. Evid Based Complement Alternat Med Research Article BACKGROUND: Medications to reduce oxidative stress are preventing cellular damage associated with hyperlipidemia. In this regard, statins (e.g., atorvastatin) act primarily by decrease in low-density lipoprotein-c but, in the last decade, hepatotoxicity, associated with liver injuries in the next months after treatments' initiation, was reported. In this case, associated phytotherapy can be a solution. PURPOSE: To investigate the antioxidant potential and response to free radicals, in the case of hyperlipidemic rats treated with atorvastatin. Sea buckthorn (Hippophae rhamnoides) and a grape extract (antioxivita) efficiency in the oxidative stress were investigated, also being ascertained the rats' organs cytoarchitecture. METHODS: Eighty-four hyperlipidemic Wistar rats were divided into seven groups and orally treated as follows: ATS, atorvastatin (20 mg/kg·bw); ATS + Hr, atorvastatin + H. rhamnoides; ATS + Aox, atorvastatin + grape extract; Hr, H. rhamnoides; and Aox, grape extract (both as 100 mg/kg·bw). HFD and Control received high fat diet and normal fodder only. After two and six months, respectively, rats were euthanized and the heart, liver, and kidneys were gathered. The tissue samples were prepared by homogenization of 0.5 g tissue, in ethanol, kept for 48 hours at 4°C–10°C and then filtered, in order to assess organs' cytoarchitecture and the TAC's values (by using cupric ion reducing antioxidant capacity (CUPRAC) assay). The test tubes were incubated, at room temperature, for 30 minutes, and then analyzed using a spectrophotometer at 450–650 nm. RESULTS: The statistics (ANOVA) revealed that sea buckthorn diminished notably (p < 0.001) the oxidative stress in the heart, liver, and kidney. After six months, the TAC's reduced levels for the heart were significant (p < 0.001) in ATS + Aox. In the case of histology, the liver's cytoarchitecture in ATS revealed abnormal cytoarchitecture. In ATS + Hr, ATS + Aox, Hr, and Aox, cell regeneration improved in different stages, especially for ATS + Hr and ATS + Aox, in comparison with HFD, which exhibited fat degeneration. Kidney's cytoarchitecture revealed cellular healing, especially in ATS + Hr and ATS + Aox. Hindawi 2020-06-22 /pmc/articles/PMC7327606/ /pubmed/32655657 http://dx.doi.org/10.1155/2020/1736803 Text en Copyright © 2020 Erieg A. Mohamed et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mohamed, Erieg A.
Bordean, Despina M.
Radulov, Isidora
Moruzi, Răzvan F.
Hulea, Călin I.
Orășan, Sergiu A.
Dumitrescu, Eugenia
Muselin, Florin
Herman, Hildegard
Brezovan, Diana
Hermenean, Anca
Cristina, Romeo T.
Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy
title Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy
title_full Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy
title_fullStr Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy
title_full_unstemmed Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy
title_short Sea Buckthorn and Grape Antioxidant Effects in Hyperlipidemic Rats: Relationship with the Atorvastatin Therapy
title_sort sea buckthorn and grape antioxidant effects in hyperlipidemic rats: relationship with the atorvastatin therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327606/
https://www.ncbi.nlm.nih.gov/pubmed/32655657
http://dx.doi.org/10.1155/2020/1736803
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