Cargando…
The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver
GATA1 is an essential transcriptional regulator of myeloid hematopoietic differentiation towards red blood cells. During erythroid differentiation, GATA1 forms different complexes with other transcription factors such as LDB1, TAL1, E2A and LMO2 (“the LDB1 complex”) or with FOG1. The functions of GA...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ferrata Storti Foundation
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327653/ https://www.ncbi.nlm.nih.gov/pubmed/31582556 http://dx.doi.org/10.3324/haematol.2019.216010 |
_version_ | 1783552587108188160 |
---|---|
author | Yu, Xiao Martella, Andrea Kolovos, Petros Stevens, Mary Stadhouders, Ralph Grosveld, Frank G. Andrieu-Soler, Charlotte |
author_facet | Yu, Xiao Martella, Andrea Kolovos, Petros Stevens, Mary Stadhouders, Ralph Grosveld, Frank G. Andrieu-Soler, Charlotte |
author_sort | Yu, Xiao |
collection | PubMed |
description | GATA1 is an essential transcriptional regulator of myeloid hematopoietic differentiation towards red blood cells. During erythroid differentiation, GATA1 forms different complexes with other transcription factors such as LDB1, TAL1, E2A and LMO2 (“the LDB1 complex”) or with FOG1. The functions of GATA1 complexes have been studied extensively in definitive erythroid differentiation; however, the temporal and spatial formation of these complexes during erythroid development is unknown. We applied proximity ligation assay (PLA) to detect, localize and quantify individual interactions during embryonic stem cell differentiation and in mouse fetal liver (FL) tissue. We show that GATA1/LDB1 interactions appear before the proerythroblast stage and increase in a subset of the CD71(+)/TER119(−) cells to activate the terminal erythroid differentiation program in 12.5 day FL. Using Ldb1 and Gata1 knockdown FL cells, we studied the functional contribution of the GATA1/LDB1 complex during differentiation. This shows that the active LDB1 complex appears quite late at the proerythroblast stage of differentiation and confirms the power of PLA in studying the dynamic interaction of proteins in cell differentiation at the single cell level. We provide dynamic insight into the temporal and spatial formation of the GATA1 and LDB1 transcription factor complexes during hematopoietic development and differentiation. |
format | Online Article Text |
id | pubmed-7327653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ferrata Storti Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-73276532020-07-10 The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver Yu, Xiao Martella, Andrea Kolovos, Petros Stevens, Mary Stadhouders, Ralph Grosveld, Frank G. Andrieu-Soler, Charlotte Haematologica Articles GATA1 is an essential transcriptional regulator of myeloid hematopoietic differentiation towards red blood cells. During erythroid differentiation, GATA1 forms different complexes with other transcription factors such as LDB1, TAL1, E2A and LMO2 (“the LDB1 complex”) or with FOG1. The functions of GATA1 complexes have been studied extensively in definitive erythroid differentiation; however, the temporal and spatial formation of these complexes during erythroid development is unknown. We applied proximity ligation assay (PLA) to detect, localize and quantify individual interactions during embryonic stem cell differentiation and in mouse fetal liver (FL) tissue. We show that GATA1/LDB1 interactions appear before the proerythroblast stage and increase in a subset of the CD71(+)/TER119(−) cells to activate the terminal erythroid differentiation program in 12.5 day FL. Using Ldb1 and Gata1 knockdown FL cells, we studied the functional contribution of the GATA1/LDB1 complex during differentiation. This shows that the active LDB1 complex appears quite late at the proerythroblast stage of differentiation and confirms the power of PLA in studying the dynamic interaction of proteins in cell differentiation at the single cell level. We provide dynamic insight into the temporal and spatial formation of the GATA1 and LDB1 transcription factor complexes during hematopoietic development and differentiation. Ferrata Storti Foundation 2020-07 /pmc/articles/PMC7327653/ /pubmed/31582556 http://dx.doi.org/10.3324/haematol.2019.216010 Text en Copyright© 2020 Ferrata Storti Foundation Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions: https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher. |
spellingShingle | Articles Yu, Xiao Martella, Andrea Kolovos, Petros Stevens, Mary Stadhouders, Ralph Grosveld, Frank G. Andrieu-Soler, Charlotte The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
title | The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
title_full | The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
title_fullStr | The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
title_full_unstemmed | The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
title_short | The dynamic emergence of GATA1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
title_sort | dynamic emergence of gata1 complexes identified in in vitro embryonic stem cell differentiation and in vivo mouse fetal liver |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327653/ https://www.ncbi.nlm.nih.gov/pubmed/31582556 http://dx.doi.org/10.3324/haematol.2019.216010 |
work_keys_str_mv | AT yuxiao thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT martellaandrea thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT kolovospetros thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT stevensmary thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT stadhoudersralph thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT grosveldfrankg thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT andrieusolercharlotte thedynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT yuxiao dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT martellaandrea dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT kolovospetros dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT stevensmary dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT stadhoudersralph dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT grosveldfrankg dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver AT andrieusolercharlotte dynamicemergenceofgata1complexesidentifiedininvitroembryonicstemcelldifferentiationandinvivomousefetalliver |