Cargando…
Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells
BACKGROUND: To investigate the anticancer effects of limonoid compounds that were isolated and purified from Xylocarpus granatum fruits on human esophageal cancer (EC) cells. A structure‐activity relationship experiment was designed to identify the functional moiety of limonoid compounds identified...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327699/ https://www.ncbi.nlm.nih.gov/pubmed/32449599 http://dx.doi.org/10.1111/1759-7714.13455 |
_version_ | 1783552597743894528 |
---|---|
author | Jing, Li Feng, Li Zhou, Zhiguo Shi, Shuai Deng, Ruoying Wang, Zhicong Liu, Yibing |
author_facet | Jing, Li Feng, Li Zhou, Zhiguo Shi, Shuai Deng, Ruoying Wang, Zhicong Liu, Yibing |
author_sort | Jing, Li |
collection | PubMed |
description | BACKGROUND: To investigate the anticancer effects of limonoid compounds that were isolated and purified from Xylocarpus granatum fruits on human esophageal cancer (EC) cells. A structure‐activity relationship experiment was designed to identify the functional moiety of limonoid compounds identified as being critical for its anticancer activity. METHODS: Eca109 cells were cultured in RPMI1640 medium and treated with limonoid compounds. Cell proliferation was determined by the MTT assay in vitro. Eca109 cells apoptosis was analyzed by by flow cytometry after being treated with xylogranatin C. The expression of p53, Bax, bcl‐2, caspase‐3 and GRP78 in Eca109 cells after xylogranatin C treatment was examined by western blot assay. RESULTS: Four linonoid compounds strongly inhibited the cellular proliferation of Eca109 cells. Xylogranatin C was the strongest inhibitor, whose inhibitory effect was comparable to that of the well‐known chemotherapeutic agent, cisplatin. Furthermore, xylogranatin C might induce Eca109 cell apoptosis through joint effects on multiple pathways, including the death receptor and endoplasmic reticulum pathways. Additionally, xylogranatin C suppressed tumor cell proliferation by upregulating miR‐203a expression in Eca109 cells. CONCLUSIONS: Xylogranatin C induced Eca109 cellular apoptosis and exerted antitumor activity. Xylogranatin C suppressed tumor cell proliferation by upregulating miR‐203a expression in Eca109 cells. |
format | Online Article Text |
id | pubmed-7327699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-73276992020-07-02 Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells Jing, Li Feng, Li Zhou, Zhiguo Shi, Shuai Deng, Ruoying Wang, Zhicong Liu, Yibing Thorac Cancer Original Articles BACKGROUND: To investigate the anticancer effects of limonoid compounds that were isolated and purified from Xylocarpus granatum fruits on human esophageal cancer (EC) cells. A structure‐activity relationship experiment was designed to identify the functional moiety of limonoid compounds identified as being critical for its anticancer activity. METHODS: Eca109 cells were cultured in RPMI1640 medium and treated with limonoid compounds. Cell proliferation was determined by the MTT assay in vitro. Eca109 cells apoptosis was analyzed by by flow cytometry after being treated with xylogranatin C. The expression of p53, Bax, bcl‐2, caspase‐3 and GRP78 in Eca109 cells after xylogranatin C treatment was examined by western blot assay. RESULTS: Four linonoid compounds strongly inhibited the cellular proliferation of Eca109 cells. Xylogranatin C was the strongest inhibitor, whose inhibitory effect was comparable to that of the well‐known chemotherapeutic agent, cisplatin. Furthermore, xylogranatin C might induce Eca109 cell apoptosis through joint effects on multiple pathways, including the death receptor and endoplasmic reticulum pathways. Additionally, xylogranatin C suppressed tumor cell proliferation by upregulating miR‐203a expression in Eca109 cells. CONCLUSIONS: Xylogranatin C induced Eca109 cellular apoptosis and exerted antitumor activity. Xylogranatin C suppressed tumor cell proliferation by upregulating miR‐203a expression in Eca109 cells. John Wiley & Sons Australia, Ltd 2020-05-25 2020-07 /pmc/articles/PMC7327699/ /pubmed/32449599 http://dx.doi.org/10.1111/1759-7714.13455 Text en © 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Jing, Li Feng, Li Zhou, Zhiguo Shi, Shuai Deng, Ruoying Wang, Zhicong Liu, Yibing Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells |
title | Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells |
title_full | Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells |
title_fullStr | Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells |
title_full_unstemmed | Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells |
title_short | Limonoid compounds from Xylocarpus granatum and their anticancer activity against esophageal cancer cells |
title_sort | limonoid compounds from xylocarpus granatum and their anticancer activity against esophageal cancer cells |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327699/ https://www.ncbi.nlm.nih.gov/pubmed/32449599 http://dx.doi.org/10.1111/1759-7714.13455 |
work_keys_str_mv | AT jingli limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells AT fengli limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells AT zhouzhiguo limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells AT shishuai limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells AT dengruoying limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells AT wangzhicong limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells AT liuyibing limonoidcompoundsfromxylocarpusgranatumandtheiranticanceractivityagainstesophagealcancercells |