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Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract
Cardiovascular diseases (CVDs) are silent killers and hyperlipidemia is a high-risk factor. Morinda citrolia leaf (MCL), which is commonly consumed by many cultural groups and has high level of catechins, might exert antihyperlipidemic properties. In this study, the catechins profile of MCL water ex...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327747/ https://www.ncbi.nlm.nih.gov/pubmed/32637711 http://dx.doi.org/10.1016/j.heliyon.2020.e04337 |
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author | Kah Hui, Chong Majid, Nurul Izwanie Mohd Yusof, Hayati Mohd Zainol, Khairi Mohamad, Habsah Mohd Zin, Zamzahaila |
author_facet | Kah Hui, Chong Majid, Nurul Izwanie Mohd Yusof, Hayati Mohd Zainol, Khairi Mohamad, Habsah Mohd Zin, Zamzahaila |
author_sort | Kah Hui, Chong |
collection | PubMed |
description | Cardiovascular diseases (CVDs) are silent killers and hyperlipidemia is a high-risk factor. Morinda citrolia leaf (MCL), which is commonly consumed by many cultural groups and has high level of catechins, might exert antihyperlipidemic properties. In this study, the catechins profile of MCL water extract was determined via HPLC and ultraperformance liquid chromatography-traveling wave ion mobility-quadrupole time of flight mass spectrometry (UPLC-TWIMS-QTOF). The major catechin in MCL and the most widely studied catechin with hypolipidemic activity, epigallocatechin gallate (EGCG), was studied in a cytotoxicity test on HepG2 cells prior the in vitro anti-hyperlipidemic assay. The total catechins of MCL reached 141.88 ± 5.04 mg/g, with catechin gallate (CG) (75.27 ± 8.49 mg/g) as the major catechin. Catechin derivatives that were identified include epigallocatechin-3-O-gallate (EGCG) with m/z 459.0912 [M + H](+), epigallocatechin (EGC) with m/z 307.0818 [M + H](+), CG with m/z 443.0976 [M + H](+), epigallocatechin(4β→8)-gallocatechin with m/z 649.0951 [M + K](+), and gallocatechin(4α→8)-epicatechin with m/z 633.1 [M + K](+). Cell inhibitions of MCL, CG and EGCG were more than IC(50) of 100 μg/ml. MCL increased LDL-c uptake up to 1.11 ± 0.03-fold, but this was insignificant relative to control. CG and EGCG significantly increased LDL-c uptake up to 1.37 ± 0.19-fold and 1.59 ± 0.19-fold, respectively. Thus, MCL with CG has shown potential for modulating hyperlipidemia. |
format | Online Article Text |
id | pubmed-7327747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73277472020-07-06 Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract Kah Hui, Chong Majid, Nurul Izwanie Mohd Yusof, Hayati Mohd Zainol, Khairi Mohamad, Habsah Mohd Zin, Zamzahaila Heliyon Article Cardiovascular diseases (CVDs) are silent killers and hyperlipidemia is a high-risk factor. Morinda citrolia leaf (MCL), which is commonly consumed by many cultural groups and has high level of catechins, might exert antihyperlipidemic properties. In this study, the catechins profile of MCL water extract was determined via HPLC and ultraperformance liquid chromatography-traveling wave ion mobility-quadrupole time of flight mass spectrometry (UPLC-TWIMS-QTOF). The major catechin in MCL and the most widely studied catechin with hypolipidemic activity, epigallocatechin gallate (EGCG), was studied in a cytotoxicity test on HepG2 cells prior the in vitro anti-hyperlipidemic assay. The total catechins of MCL reached 141.88 ± 5.04 mg/g, with catechin gallate (CG) (75.27 ± 8.49 mg/g) as the major catechin. Catechin derivatives that were identified include epigallocatechin-3-O-gallate (EGCG) with m/z 459.0912 [M + H](+), epigallocatechin (EGC) with m/z 307.0818 [M + H](+), CG with m/z 443.0976 [M + H](+), epigallocatechin(4β→8)-gallocatechin with m/z 649.0951 [M + K](+), and gallocatechin(4α→8)-epicatechin with m/z 633.1 [M + K](+). Cell inhibitions of MCL, CG and EGCG were more than IC(50) of 100 μg/ml. MCL increased LDL-c uptake up to 1.11 ± 0.03-fold, but this was insignificant relative to control. CG and EGCG significantly increased LDL-c uptake up to 1.37 ± 0.19-fold and 1.59 ± 0.19-fold, respectively. Thus, MCL with CG has shown potential for modulating hyperlipidemia. Elsevier 2020-06-28 /pmc/articles/PMC7327747/ /pubmed/32637711 http://dx.doi.org/10.1016/j.heliyon.2020.e04337 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Kah Hui, Chong Majid, Nurul Izwanie Mohd Yusof, Hayati Mohd Zainol, Khairi Mohamad, Habsah Mohd Zin, Zamzahaila Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract |
title | Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract |
title_full | Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract |
title_fullStr | Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract |
title_full_unstemmed | Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract |
title_short | Catechin profile and hypolipidemic activity of Morinda citrifolia leaf water extract |
title_sort | catechin profile and hypolipidemic activity of morinda citrifolia leaf water extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327747/ https://www.ncbi.nlm.nih.gov/pubmed/32637711 http://dx.doi.org/10.1016/j.heliyon.2020.e04337 |
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