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Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly

Centriole assembly requires a small number of conserved proteins. The precise pathway of centriole assembly has been difficult to study, as the lack of any one of the core assembly proteins [Plk4, Ana2 (the homologue of mammalian STIL), Sas-6, Sas-4 (mammalian CPAP) or Asl (mammalian Cep152)] leads...

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Autores principales: Gartenmann, Lisa, Vicente, Catarina C., Wainman, Alan, Novak, Zsofi A., Sieber, Boris, Richens, Jennifer H., Raff, Jordan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328145/
https://www.ncbi.nlm.nih.gov/pubmed/32409564
http://dx.doi.org/10.1242/jcs.244574
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author Gartenmann, Lisa
Vicente, Catarina C.
Wainman, Alan
Novak, Zsofi A.
Sieber, Boris
Richens, Jennifer H.
Raff, Jordan W.
author_facet Gartenmann, Lisa
Vicente, Catarina C.
Wainman, Alan
Novak, Zsofi A.
Sieber, Boris
Richens, Jennifer H.
Raff, Jordan W.
author_sort Gartenmann, Lisa
collection PubMed
description Centriole assembly requires a small number of conserved proteins. The precise pathway of centriole assembly has been difficult to study, as the lack of any one of the core assembly proteins [Plk4, Ana2 (the homologue of mammalian STIL), Sas-6, Sas-4 (mammalian CPAP) or Asl (mammalian Cep152)] leads to the absence of centrioles. Here, we use Sas-6 and Ana2 particles (SAPs) as a new model to probe the pathway of centriole and centrosome assembly. SAPs form in Drosophila eggs or embryos when Sas-6 and Ana2 are overexpressed. SAP assembly requires Sas-4, but not Plk4, whereas Asl helps to initiate SAP assembly but is not required for SAP growth. Although not centrioles, SAPs recruit and organise many centriole and centrosome components, nucleate microtubules, organise actin structures and compete with endogenous centrosomes to form mitotic spindle poles. SAPs require Asl to efficiently recruit pericentriolar material (PCM), but Spd-2 (the homologue of mammalian Cep192) can promote some PCM assembly independently of Asl. These observations provide new insights into the pathways of centriole and centrosome assembly.
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spelling pubmed-73281452020-07-10 Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly Gartenmann, Lisa Vicente, Catarina C. Wainman, Alan Novak, Zsofi A. Sieber, Boris Richens, Jennifer H. Raff, Jordan W. J Cell Sci Research Article Centriole assembly requires a small number of conserved proteins. The precise pathway of centriole assembly has been difficult to study, as the lack of any one of the core assembly proteins [Plk4, Ana2 (the homologue of mammalian STIL), Sas-6, Sas-4 (mammalian CPAP) or Asl (mammalian Cep152)] leads to the absence of centrioles. Here, we use Sas-6 and Ana2 particles (SAPs) as a new model to probe the pathway of centriole and centrosome assembly. SAPs form in Drosophila eggs or embryos when Sas-6 and Ana2 are overexpressed. SAP assembly requires Sas-4, but not Plk4, whereas Asl helps to initiate SAP assembly but is not required for SAP growth. Although not centrioles, SAPs recruit and organise many centriole and centrosome components, nucleate microtubules, organise actin structures and compete with endogenous centrosomes to form mitotic spindle poles. SAPs require Asl to efficiently recruit pericentriolar material (PCM), but Spd-2 (the homologue of mammalian Cep192) can promote some PCM assembly independently of Asl. These observations provide new insights into the pathways of centriole and centrosome assembly. The Company of Biologists Ltd 2020-06-22 /pmc/articles/PMC7328145/ /pubmed/32409564 http://dx.doi.org/10.1242/jcs.244574 Text en © 2020. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Gartenmann, Lisa
Vicente, Catarina C.
Wainman, Alan
Novak, Zsofi A.
Sieber, Boris
Richens, Jennifer H.
Raff, Jordan W.
Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
title Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
title_full Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
title_fullStr Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
title_full_unstemmed Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
title_short Drosophila Sas-6, Ana2 and Sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
title_sort drosophila sas-6, ana2 and sas-4 self-organise into macromolecular structures that can be used to probe centriole and centrosome assembly
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7328145/
https://www.ncbi.nlm.nih.gov/pubmed/32409564
http://dx.doi.org/10.1242/jcs.244574
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