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Isolation and characterization of new Puumala orthohantavirus strains from Germany
Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city Osnabrück, north-west Germany. Coding and non-coding sequences of all th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329759/ https://www.ncbi.nlm.nih.gov/pubmed/32328924 http://dx.doi.org/10.1007/s11262-020-01755-3 |
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author | Binder, Florian Reiche, Sven Roman-Sosa, Gleyder Saathoff, Marion Ryll, René Trimpert, Jakob Kunec, Dusan Höper, Dirk Ulrich, Rainer G. |
author_facet | Binder, Florian Reiche, Sven Roman-Sosa, Gleyder Saathoff, Marion Ryll, René Trimpert, Jakob Kunec, Dusan Höper, Dirk Ulrich, Rainer G. |
author_sort | Binder, Florian |
collection | PubMed |
description | Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city Osnabrück, north-west Germany. Coding and non-coding sequences of all three segments (S, M, and L) were determined from original lung tissue, after isolation and after additional passaging in VeroE6 cells and a bank vole-derived kidney cell line. Different single amino acid substitutions were observed in the RNA-dependent RNA polymerase (RdRP) of the two stable PUUV isolates. The PUUV strain from VeroE6 cells showed a lower titer when propagated on bank vole cells compared to VeroE6 cells. Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. In conclusion, this is the first isolation of a PUUV strain from Central Europe and the generation of glycoprotein-specific monoclonal antibodies for this PUUV isolate. The obtained virus isolate and GPC-specific antibodies are instrumental tools for future reservoir host studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11262-020-01755-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7329759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-73297592020-07-07 Isolation and characterization of new Puumala orthohantavirus strains from Germany Binder, Florian Reiche, Sven Roman-Sosa, Gleyder Saathoff, Marion Ryll, René Trimpert, Jakob Kunec, Dusan Höper, Dirk Ulrich, Rainer G. Virus Genes Original Paper Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city Osnabrück, north-west Germany. Coding and non-coding sequences of all three segments (S, M, and L) were determined from original lung tissue, after isolation and after additional passaging in VeroE6 cells and a bank vole-derived kidney cell line. Different single amino acid substitutions were observed in the RNA-dependent RNA polymerase (RdRP) of the two stable PUUV isolates. The PUUV strain from VeroE6 cells showed a lower titer when propagated on bank vole cells compared to VeroE6 cells. Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. In conclusion, this is the first isolation of a PUUV strain from Central Europe and the generation of glycoprotein-specific monoclonal antibodies for this PUUV isolate. The obtained virus isolate and GPC-specific antibodies are instrumental tools for future reservoir host studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11262-020-01755-3) contains supplementary material, which is available to authorized users. Springer US 2020-04-23 2020 /pmc/articles/PMC7329759/ /pubmed/32328924 http://dx.doi.org/10.1007/s11262-020-01755-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Paper Binder, Florian Reiche, Sven Roman-Sosa, Gleyder Saathoff, Marion Ryll, René Trimpert, Jakob Kunec, Dusan Höper, Dirk Ulrich, Rainer G. Isolation and characterization of new Puumala orthohantavirus strains from Germany |
title | Isolation and characterization of new Puumala orthohantavirus strains from Germany |
title_full | Isolation and characterization of new Puumala orthohantavirus strains from Germany |
title_fullStr | Isolation and characterization of new Puumala orthohantavirus strains from Germany |
title_full_unstemmed | Isolation and characterization of new Puumala orthohantavirus strains from Germany |
title_short | Isolation and characterization of new Puumala orthohantavirus strains from Germany |
title_sort | isolation and characterization of new puumala orthohantavirus strains from germany |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329759/ https://www.ncbi.nlm.nih.gov/pubmed/32328924 http://dx.doi.org/10.1007/s11262-020-01755-3 |
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