Cargando…
Analysis of non-conjugated steroids in water using paper spray mass spectrometry
A novel strategy for the direct analysis of non-conjugated steroids in water using paper spray mass spectrometry (PS-MS) has been developed. PS-MS was used in the identification and quantification of non-conjugated (free) steroids in fish tank water samples. Data shown herein indicates that individu...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329809/ https://www.ncbi.nlm.nih.gov/pubmed/32612114 http://dx.doi.org/10.1038/s41598-020-67484-7 |
_version_ | 1783552974672363520 |
---|---|
author | Jjunju, Fred. P. M. Damon, Deidre E. Romero-Perez, David Young, Iain S. Ward, Ryan J. Marshall, Alan Maher, Simon Badu-Tawiah, Abraham K. |
author_facet | Jjunju, Fred. P. M. Damon, Deidre E. Romero-Perez, David Young, Iain S. Ward, Ryan J. Marshall, Alan Maher, Simon Badu-Tawiah, Abraham K. |
author_sort | Jjunju, Fred. P. M. |
collection | PubMed |
description | A novel strategy for the direct analysis of non-conjugated steroids in water using paper spray mass spectrometry (PS-MS) has been developed. PS-MS was used in the identification and quantification of non-conjugated (free) steroids in fish tank water samples. Data shown herein indicates that individual amounts of free steroids can be detected in aqua as low as; 0.17 ng/µL, 0.039 ng/µL, 0.43 ng/µL, 0.0076 ng/µL for aldosterone, corticosterone, cortisol, and β-estrone, respectively, and with an average relative standard deviation of ca. < 10% in the positive ion mode using PS-MS/MS. Direct detection of free steroids in a raw water mixture, from aquaculture, without prior sample preparation is demonstrated. The presence of free steroids released in fish water samples was confirmed via tandem mass spectrometry using collision-induced dissociation. This approach shows promise for rapid and direct water quality monitoring to provide a holistic assessment of non-conjugated steroids in aqua. |
format | Online Article Text |
id | pubmed-7329809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73298092020-07-06 Analysis of non-conjugated steroids in water using paper spray mass spectrometry Jjunju, Fred. P. M. Damon, Deidre E. Romero-Perez, David Young, Iain S. Ward, Ryan J. Marshall, Alan Maher, Simon Badu-Tawiah, Abraham K. Sci Rep Article A novel strategy for the direct analysis of non-conjugated steroids in water using paper spray mass spectrometry (PS-MS) has been developed. PS-MS was used in the identification and quantification of non-conjugated (free) steroids in fish tank water samples. Data shown herein indicates that individual amounts of free steroids can be detected in aqua as low as; 0.17 ng/µL, 0.039 ng/µL, 0.43 ng/µL, 0.0076 ng/µL for aldosterone, corticosterone, cortisol, and β-estrone, respectively, and with an average relative standard deviation of ca. < 10% in the positive ion mode using PS-MS/MS. Direct detection of free steroids in a raw water mixture, from aquaculture, without prior sample preparation is demonstrated. The presence of free steroids released in fish water samples was confirmed via tandem mass spectrometry using collision-induced dissociation. This approach shows promise for rapid and direct water quality monitoring to provide a holistic assessment of non-conjugated steroids in aqua. Nature Publishing Group UK 2020-07-01 /pmc/articles/PMC7329809/ /pubmed/32612114 http://dx.doi.org/10.1038/s41598-020-67484-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jjunju, Fred. P. M. Damon, Deidre E. Romero-Perez, David Young, Iain S. Ward, Ryan J. Marshall, Alan Maher, Simon Badu-Tawiah, Abraham K. Analysis of non-conjugated steroids in water using paper spray mass spectrometry |
title | Analysis of non-conjugated steroids in water using paper spray mass spectrometry |
title_full | Analysis of non-conjugated steroids in water using paper spray mass spectrometry |
title_fullStr | Analysis of non-conjugated steroids in water using paper spray mass spectrometry |
title_full_unstemmed | Analysis of non-conjugated steroids in water using paper spray mass spectrometry |
title_short | Analysis of non-conjugated steroids in water using paper spray mass spectrometry |
title_sort | analysis of non-conjugated steroids in water using paper spray mass spectrometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329809/ https://www.ncbi.nlm.nih.gov/pubmed/32612114 http://dx.doi.org/10.1038/s41598-020-67484-7 |
work_keys_str_mv | AT jjunjufredpm analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT damondeidree analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT romeroperezdavid analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT youngiains analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT wardryanj analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT marshallalan analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT mahersimon analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry AT badutawiahabrahamk analysisofnonconjugatedsteroidsinwaterusingpaperspraymassspectrometry |