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miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum
Cellular transitions during development and stress response depend on coordinated transcriptomic and proteomic alterations. Pollen is particular because its development is a complex process that includes meiotic and mitotic divisions which causes a high heat sensitivity of these cells. Development a...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329895/ https://www.ncbi.nlm.nih.gov/pubmed/32612181 http://dx.doi.org/10.1038/s41598-020-67833-6 |
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author | Keller, Mario Schleiff, Enrico Simm, Stefan |
author_facet | Keller, Mario Schleiff, Enrico Simm, Stefan |
author_sort | Keller, Mario |
collection | PubMed |
description | Cellular transitions during development and stress response depend on coordinated transcriptomic and proteomic alterations. Pollen is particular because its development is a complex process that includes meiotic and mitotic divisions which causes a high heat sensitivity of these cells. Development and stress response are accompanied by a reprogramming of the transcriptome, e.g. by post-transcriptional regulation via miRNAs. We identified known and potentially novel miRNAs in the transcriptome of developing and heat-stressed pollen of Solanum lycopersicum (tomato). The prediction of target mRNAs yielded an equal number of predicted target-sites in CDS and 3′UTR regions of target mRNAs. The result enabled the postulation of a possible link between miRNAs and a fine-tuning of transcription factor abundance during pollen development. miRNAs seem to play a role in the pollen heat stress response as well. We identified several heat stress transcription factors and heat shock proteins as putative targets of miRNAs in response to heat stress, thereby placing these miRNAs as important elements of thermotolerance. Moreover, for members of the AP2, SBP and ARF family members we could predict a miRNA-mediated regulation during development via the miR172, mir156 and mir160-family strengthening the current concept of a cross-connection between development and stress response in plants. |
format | Online Article Text |
id | pubmed-7329895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73298952020-07-06 miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum Keller, Mario Schleiff, Enrico Simm, Stefan Sci Rep Article Cellular transitions during development and stress response depend on coordinated transcriptomic and proteomic alterations. Pollen is particular because its development is a complex process that includes meiotic and mitotic divisions which causes a high heat sensitivity of these cells. Development and stress response are accompanied by a reprogramming of the transcriptome, e.g. by post-transcriptional regulation via miRNAs. We identified known and potentially novel miRNAs in the transcriptome of developing and heat-stressed pollen of Solanum lycopersicum (tomato). The prediction of target mRNAs yielded an equal number of predicted target-sites in CDS and 3′UTR regions of target mRNAs. The result enabled the postulation of a possible link between miRNAs and a fine-tuning of transcription factor abundance during pollen development. miRNAs seem to play a role in the pollen heat stress response as well. We identified several heat stress transcription factors and heat shock proteins as putative targets of miRNAs in response to heat stress, thereby placing these miRNAs as important elements of thermotolerance. Moreover, for members of the AP2, SBP and ARF family members we could predict a miRNA-mediated regulation during development via the miR172, mir156 and mir160-family strengthening the current concept of a cross-connection between development and stress response in plants. Nature Publishing Group UK 2020-07-01 /pmc/articles/PMC7329895/ /pubmed/32612181 http://dx.doi.org/10.1038/s41598-020-67833-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Keller, Mario Schleiff, Enrico Simm, Stefan miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum |
title | miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum |
title_full | miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum |
title_fullStr | miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum |
title_full_unstemmed | miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum |
title_short | miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum |
title_sort | mirnas involved in transcriptome remodeling during pollen development and heat stress response in solanum lycopersicum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329895/ https://www.ncbi.nlm.nih.gov/pubmed/32612181 http://dx.doi.org/10.1038/s41598-020-67833-6 |
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