Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling

L-myo-inositol phosphate synthase (MIPS; EC 5.5.1.4) is involved in abiotic stress tolerance, however its disruption and overexpression has also been associated with enhanced tolerance to pathogens. The molecular mechanism underlying the role of MIPS in growth, immunity and abiotic stress tolerance...

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Autores principales: Sharma, Naveen, Chaudhary, Chanderkant, Khurana, Paramjit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329911/
https://www.ncbi.nlm.nih.gov/pubmed/32612188
http://dx.doi.org/10.1038/s41598-020-67627-w
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author Sharma, Naveen
Chaudhary, Chanderkant
Khurana, Paramjit
author_facet Sharma, Naveen
Chaudhary, Chanderkant
Khurana, Paramjit
author_sort Sharma, Naveen
collection PubMed
description L-myo-inositol phosphate synthase (MIPS; EC 5.5.1.4) is involved in abiotic stress tolerance, however its disruption and overexpression has also been associated with enhanced tolerance to pathogens. The molecular mechanism underlying the role of MIPS in growth, immunity and abiotic stress tolerance remains uncharacterized. We explore the molecular mechanism of MIPS action during growth and heat stress conditions. We raised and characterized the TaMIPS over-expressing rice transgenics which showed a reduced reproductive potential. Transcriptome analysis of overexpression transgenics revealed the activation of ET/JA dependent immune response. Pull-down analysis revealed the interaction of TaMIPS-B with ethylene related proteins. Our results suggest an essential requirement of MIPS for mediating the ethylene response and regulate the growth. A model is proposed outlining how fine tuning of MIPS regulate growth and stress tolerance of the plant.
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spelling pubmed-73299112020-07-06 Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling Sharma, Naveen Chaudhary, Chanderkant Khurana, Paramjit Sci Rep Article L-myo-inositol phosphate synthase (MIPS; EC 5.5.1.4) is involved in abiotic stress tolerance, however its disruption and overexpression has also been associated with enhanced tolerance to pathogens. The molecular mechanism underlying the role of MIPS in growth, immunity and abiotic stress tolerance remains uncharacterized. We explore the molecular mechanism of MIPS action during growth and heat stress conditions. We raised and characterized the TaMIPS over-expressing rice transgenics which showed a reduced reproductive potential. Transcriptome analysis of overexpression transgenics revealed the activation of ET/JA dependent immune response. Pull-down analysis revealed the interaction of TaMIPS-B with ethylene related proteins. Our results suggest an essential requirement of MIPS for mediating the ethylene response and regulate the growth. A model is proposed outlining how fine tuning of MIPS regulate growth and stress tolerance of the plant. Nature Publishing Group UK 2020-07-01 /pmc/articles/PMC7329911/ /pubmed/32612188 http://dx.doi.org/10.1038/s41598-020-67627-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sharma, Naveen
Chaudhary, Chanderkant
Khurana, Paramjit
Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
title Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
title_full Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
title_fullStr Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
title_full_unstemmed Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
title_short Wheat Myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
title_sort wheat myo-inositol phosphate synthase influences plant growth and stress responses via ethylene mediated signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7329911/
https://www.ncbi.nlm.nih.gov/pubmed/32612188
http://dx.doi.org/10.1038/s41598-020-67627-w
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