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Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract

Erythroxylum cuneatum (E. cuneatum) which belongs to Erythroxylaceae family is a tropical flowering plant from the genus of Erythroxylum. It is used in Malaysia and Thailand's traditional medicines, yet there is limited scientific reports on its medicinal value. This study aimed at exploring th...

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Autores principales: Li, Lim Seow, Chiroma, Samaila Musa, Hashim, Thuaibah, Adam, Siti Khadijah, Mohd Moklas, Mohamad Aris, Yusuf, Zauyah, Rahman, Shamima Abdul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330067/
https://www.ncbi.nlm.nih.gov/pubmed/32637674
http://dx.doi.org/10.1016/j.heliyon.2020.e04141
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author Li, Lim Seow
Chiroma, Samaila Musa
Hashim, Thuaibah
Adam, Siti Khadijah
Mohd Moklas, Mohamad Aris
Yusuf, Zauyah
Rahman, Shamima Abdul
author_facet Li, Lim Seow
Chiroma, Samaila Musa
Hashim, Thuaibah
Adam, Siti Khadijah
Mohd Moklas, Mohamad Aris
Yusuf, Zauyah
Rahman, Shamima Abdul
author_sort Li, Lim Seow
collection PubMed
description Erythroxylum cuneatum (E. cuneatum) which belongs to Erythroxylaceae family is a tropical flowering plant from the genus of Erythroxylum. It is used in Malaysia and Thailand's traditional medicines, yet there is limited scientific reports on its medicinal value. This study aimed at exploring the antioxidative and anti-inflammatory properties of E. cuneatum alkaloid leaf extract. The alkaloid extract was obtained through Soxhlet heat extraction method, while the antioxidantive properties were assessed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferric reducing antioxidant power (FRAP) and xanthine oxidase inhibition (XOI) assays. Further, anti-inflammatory property of the extract was evaluated on rat's model of carrageenan induced paw model of edema via physical measurements and histology. The extract exhibited antioxidant activity with an EC(50) value of 1482 μg/ml in the DPPH radical scavenging assay, an EC(1) value of 2191 μg/ml in the FRAP assay and 10.15 ± 6.20% in the XOI assay. Rats pretreated with the extract have shown dose dependent decrease in paw edema when compared to non-treated group of rats. The highest dose (50 mg/kg) of extract exhibited similar effects to aspirin in terms of reducing paw thickness, leucocytes infiltration and disruption of collagen. In conclusion, the E. cuneatum alkaloid leaf extract possesses both antioxidative and anti-inflammatory properties suggesting its potentials for future development of antioxidant and anti-inflammatory drugs.
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spelling pubmed-73300672020-07-06 Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract Li, Lim Seow Chiroma, Samaila Musa Hashim, Thuaibah Adam, Siti Khadijah Mohd Moklas, Mohamad Aris Yusuf, Zauyah Rahman, Shamima Abdul Heliyon Article Erythroxylum cuneatum (E. cuneatum) which belongs to Erythroxylaceae family is a tropical flowering plant from the genus of Erythroxylum. It is used in Malaysia and Thailand's traditional medicines, yet there is limited scientific reports on its medicinal value. This study aimed at exploring the antioxidative and anti-inflammatory properties of E. cuneatum alkaloid leaf extract. The alkaloid extract was obtained through Soxhlet heat extraction method, while the antioxidantive properties were assessed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferric reducing antioxidant power (FRAP) and xanthine oxidase inhibition (XOI) assays. Further, anti-inflammatory property of the extract was evaluated on rat's model of carrageenan induced paw model of edema via physical measurements and histology. The extract exhibited antioxidant activity with an EC(50) value of 1482 μg/ml in the DPPH radical scavenging assay, an EC(1) value of 2191 μg/ml in the FRAP assay and 10.15 ± 6.20% in the XOI assay. Rats pretreated with the extract have shown dose dependent decrease in paw edema when compared to non-treated group of rats. The highest dose (50 mg/kg) of extract exhibited similar effects to aspirin in terms of reducing paw thickness, leucocytes infiltration and disruption of collagen. In conclusion, the E. cuneatum alkaloid leaf extract possesses both antioxidative and anti-inflammatory properties suggesting its potentials for future development of antioxidant and anti-inflammatory drugs. Elsevier 2020-06-28 /pmc/articles/PMC7330067/ /pubmed/32637674 http://dx.doi.org/10.1016/j.heliyon.2020.e04141 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Lim Seow
Chiroma, Samaila Musa
Hashim, Thuaibah
Adam, Siti Khadijah
Mohd Moklas, Mohamad Aris
Yusuf, Zauyah
Rahman, Shamima Abdul
Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
title Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
title_full Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
title_fullStr Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
title_full_unstemmed Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
title_short Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
title_sort antioxidant and anti-inflammatory properties of erythroxylum cuneatum alkaloid leaf extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330067/
https://www.ncbi.nlm.nih.gov/pubmed/32637674
http://dx.doi.org/10.1016/j.heliyon.2020.e04141
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