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Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract
Erythroxylum cuneatum (E. cuneatum) which belongs to Erythroxylaceae family is a tropical flowering plant from the genus of Erythroxylum. It is used in Malaysia and Thailand's traditional medicines, yet there is limited scientific reports on its medicinal value. This study aimed at exploring th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330067/ https://www.ncbi.nlm.nih.gov/pubmed/32637674 http://dx.doi.org/10.1016/j.heliyon.2020.e04141 |
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author | Li, Lim Seow Chiroma, Samaila Musa Hashim, Thuaibah Adam, Siti Khadijah Mohd Moklas, Mohamad Aris Yusuf, Zauyah Rahman, Shamima Abdul |
author_facet | Li, Lim Seow Chiroma, Samaila Musa Hashim, Thuaibah Adam, Siti Khadijah Mohd Moklas, Mohamad Aris Yusuf, Zauyah Rahman, Shamima Abdul |
author_sort | Li, Lim Seow |
collection | PubMed |
description | Erythroxylum cuneatum (E. cuneatum) which belongs to Erythroxylaceae family is a tropical flowering plant from the genus of Erythroxylum. It is used in Malaysia and Thailand's traditional medicines, yet there is limited scientific reports on its medicinal value. This study aimed at exploring the antioxidative and anti-inflammatory properties of E. cuneatum alkaloid leaf extract. The alkaloid extract was obtained through Soxhlet heat extraction method, while the antioxidantive properties were assessed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferric reducing antioxidant power (FRAP) and xanthine oxidase inhibition (XOI) assays. Further, anti-inflammatory property of the extract was evaluated on rat's model of carrageenan induced paw model of edema via physical measurements and histology. The extract exhibited antioxidant activity with an EC(50) value of 1482 μg/ml in the DPPH radical scavenging assay, an EC(1) value of 2191 μg/ml in the FRAP assay and 10.15 ± 6.20% in the XOI assay. Rats pretreated with the extract have shown dose dependent decrease in paw edema when compared to non-treated group of rats. The highest dose (50 mg/kg) of extract exhibited similar effects to aspirin in terms of reducing paw thickness, leucocytes infiltration and disruption of collagen. In conclusion, the E. cuneatum alkaloid leaf extract possesses both antioxidative and anti-inflammatory properties suggesting its potentials for future development of antioxidant and anti-inflammatory drugs. |
format | Online Article Text |
id | pubmed-7330067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73300672020-07-06 Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract Li, Lim Seow Chiroma, Samaila Musa Hashim, Thuaibah Adam, Siti Khadijah Mohd Moklas, Mohamad Aris Yusuf, Zauyah Rahman, Shamima Abdul Heliyon Article Erythroxylum cuneatum (E. cuneatum) which belongs to Erythroxylaceae family is a tropical flowering plant from the genus of Erythroxylum. It is used in Malaysia and Thailand's traditional medicines, yet there is limited scientific reports on its medicinal value. This study aimed at exploring the antioxidative and anti-inflammatory properties of E. cuneatum alkaloid leaf extract. The alkaloid extract was obtained through Soxhlet heat extraction method, while the antioxidantive properties were assessed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, ferric reducing antioxidant power (FRAP) and xanthine oxidase inhibition (XOI) assays. Further, anti-inflammatory property of the extract was evaluated on rat's model of carrageenan induced paw model of edema via physical measurements and histology. The extract exhibited antioxidant activity with an EC(50) value of 1482 μg/ml in the DPPH radical scavenging assay, an EC(1) value of 2191 μg/ml in the FRAP assay and 10.15 ± 6.20% in the XOI assay. Rats pretreated with the extract have shown dose dependent decrease in paw edema when compared to non-treated group of rats. The highest dose (50 mg/kg) of extract exhibited similar effects to aspirin in terms of reducing paw thickness, leucocytes infiltration and disruption of collagen. In conclusion, the E. cuneatum alkaloid leaf extract possesses both antioxidative and anti-inflammatory properties suggesting its potentials for future development of antioxidant and anti-inflammatory drugs. Elsevier 2020-06-28 /pmc/articles/PMC7330067/ /pubmed/32637674 http://dx.doi.org/10.1016/j.heliyon.2020.e04141 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Lim Seow Chiroma, Samaila Musa Hashim, Thuaibah Adam, Siti Khadijah Mohd Moklas, Mohamad Aris Yusuf, Zauyah Rahman, Shamima Abdul Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract |
title | Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract |
title_full | Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract |
title_fullStr | Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract |
title_full_unstemmed | Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract |
title_short | Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract |
title_sort | antioxidant and anti-inflammatory properties of erythroxylum cuneatum alkaloid leaf extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330067/ https://www.ncbi.nlm.nih.gov/pubmed/32637674 http://dx.doi.org/10.1016/j.heliyon.2020.e04141 |
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