An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020

We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributabl...

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Detalles Bibliográficos
Autores principales: Monecke, Stefan, König, Elisabeth, Earls, Megan R, Leitner, Eva, Müller, Elke, Wagner, Gabriel E, Poitz, David M, Jatzwauk, Lutz, Vremerǎ, Teodora, Dorneanu, Olivia S, Simbeck, Alexandra, Ambrosch, Andreas, Zollner-Schwetz, Ines, Krause, Robert, Ruppitsch, Werner, Schneider-Brachert, Wulf, Coleman, David C, Steinmetz, Ivo, Ehricht, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Centre for Disease Prevention and Control (ECDC) 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331142/
https://www.ncbi.nlm.nih.gov/pubmed/32613938
http://dx.doi.org/10.2807/1560-7917.ES.2020.25.25.2000929
Descripción
Sumario:We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.

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