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An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020
We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributabl...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
European Centre for Disease Prevention and Control (ECDC)
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331142/ https://www.ncbi.nlm.nih.gov/pubmed/32613938 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.25.2000929 |
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| author | Monecke, Stefan König, Elisabeth Earls, Megan R Leitner, Eva Müller, Elke Wagner, Gabriel E Poitz, David M Jatzwauk, Lutz Vremerǎ, Teodora Dorneanu, Olivia S Simbeck, Alexandra Ambrosch, Andreas Zollner-Schwetz, Ines Krause, Robert Ruppitsch, Werner Schneider-Brachert, Wulf Coleman, David C Steinmetz, Ivo Ehricht, Ralf |
| author_facet | Monecke, Stefan König, Elisabeth Earls, Megan R Leitner, Eva Müller, Elke Wagner, Gabriel E Poitz, David M Jatzwauk, Lutz Vremerǎ, Teodora Dorneanu, Olivia S Simbeck, Alexandra Ambrosch, Andreas Zollner-Schwetz, Ines Krause, Robert Ruppitsch, Werner Schneider-Brachert, Wulf Coleman, David C Steinmetz, Ivo Ehricht, Ralf |
| author_sort | Monecke, Stefan |
| collection | PubMed |
| description | We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites. |
| format | Online Article Text |
| id | pubmed-7331142 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | European Centre for Disease Prevention and Control (ECDC) |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-73311422020-07-06 An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 Monecke, Stefan König, Elisabeth Earls, Megan R Leitner, Eva Müller, Elke Wagner, Gabriel E Poitz, David M Jatzwauk, Lutz Vremerǎ, Teodora Dorneanu, Olivia S Simbeck, Alexandra Ambrosch, Andreas Zollner-Schwetz, Ines Krause, Robert Ruppitsch, Werner Schneider-Brachert, Wulf Coleman, David C Steinmetz, Ivo Ehricht, Ralf Euro Surveill Rapid Communication We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites. European Centre for Disease Prevention and Control (ECDC) 2020-06-25 /pmc/articles/PMC7331142/ /pubmed/32613938 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.25.2000929 Text en This article is copyright of the authors or their affiliated institutions, 2020. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
| spellingShingle | Rapid Communication Monecke, Stefan König, Elisabeth Earls, Megan R Leitner, Eva Müller, Elke Wagner, Gabriel E Poitz, David M Jatzwauk, Lutz Vremerǎ, Teodora Dorneanu, Olivia S Simbeck, Alexandra Ambrosch, Andreas Zollner-Schwetz, Ines Krause, Robert Ruppitsch, Werner Schneider-Brachert, Wulf Coleman, David C Steinmetz, Ivo Ehricht, Ralf An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 |
| title | An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 |
| title_full | An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 |
| title_fullStr | An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 |
| title_full_unstemmed | An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 |
| title_short | An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020 |
| title_sort | epidemic cc1-mrsa-iv clone yields false-negative test results in molecular mrsa identification assays: a note of caution, austria, germany, ireland, 2020 |
| topic | Rapid Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331142/ https://www.ncbi.nlm.nih.gov/pubmed/32613938 http://dx.doi.org/10.2807/1560-7917.ES.2020.25.25.2000929 |
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