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Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders
BACKGROUND: Adipose derived stem cells (ADSCs) are clinically widely used somatic stem cells obtained from white adipose tissue. They are characterized by ability to differentiate e.g. into osteoblasts and might successfully regenerate bone tissue in fracture repair. However, the main problem of som...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331176/ https://www.ncbi.nlm.nih.gov/pubmed/32615920 http://dx.doi.org/10.1186/s10020-020-00192-0 |
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author | Skubis-Sikora, Aleksandra Sikora, Bartosz Witkowska, Agnieszka Mazurek, Urszula Gola, Joanna |
author_facet | Skubis-Sikora, Aleksandra Sikora, Bartosz Witkowska, Agnieszka Mazurek, Urszula Gola, Joanna |
author_sort | Skubis-Sikora, Aleksandra |
collection | PubMed |
description | BACKGROUND: Adipose derived stem cells (ADSCs) are clinically widely used somatic stem cells obtained from white adipose tissue. They are characterized by ability to differentiate e.g. into osteoblasts and might successfully regenerate bone tissue in fracture repair. However, the main problem of somatic stem cells is a documented influence of various diseases, drugs or age which can inhibit cells activity. Therefore, in the present study, we investigated the influence of insulin resistance (IR) and type 2 diabetes (T2D) on the proliferation and differentiation potential of ADSCs. METHODS: The fat from subcutaneous abdominal adipose tissue was acquired by lipoaspiration from 23 voluntary participants, divided into three groups: with diabetes type 2, with insulin resistance and control healthy donors. The proliferative potential was analyzed by cell cytotoxicity assays and by mRNA expression of genes connected with proliferation. Flow cytometry was done for identifying proteins characteristic for mesenchymal stem cells and an analysis of osteogenic differentiation potential based on the assessment of osteogenic markers by real time RT-qPCR, and the evaluation of calcium deposition were also performed. RESULTS: The results showed that diabetes type 2 lowered the activity of ADSCs in proliferation assays and changed their phenotypical characteristics. Interestingly, we observed differences in the proliferation potential of ADSCs in patients with insulin resistance, which is often the first phase of diabetes, compared to the control. It might suggest that insulin resistance, early-stage T2D, alters the activity of cells. Moreover, expression of osteogenesis markers was higher in cells from T2D patients than in cells from patients with IR and control. CONCLUSION: We conclude that type 2 diabetes changes the activity of stem cells, and insulin resistance influences on the proliferation of ADSCs. |
format | Online Article Text |
id | pubmed-7331176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73311762020-07-06 Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders Skubis-Sikora, Aleksandra Sikora, Bartosz Witkowska, Agnieszka Mazurek, Urszula Gola, Joanna Mol Med Research Article BACKGROUND: Adipose derived stem cells (ADSCs) are clinically widely used somatic stem cells obtained from white adipose tissue. They are characterized by ability to differentiate e.g. into osteoblasts and might successfully regenerate bone tissue in fracture repair. However, the main problem of somatic stem cells is a documented influence of various diseases, drugs or age which can inhibit cells activity. Therefore, in the present study, we investigated the influence of insulin resistance (IR) and type 2 diabetes (T2D) on the proliferation and differentiation potential of ADSCs. METHODS: The fat from subcutaneous abdominal adipose tissue was acquired by lipoaspiration from 23 voluntary participants, divided into three groups: with diabetes type 2, with insulin resistance and control healthy donors. The proliferative potential was analyzed by cell cytotoxicity assays and by mRNA expression of genes connected with proliferation. Flow cytometry was done for identifying proteins characteristic for mesenchymal stem cells and an analysis of osteogenic differentiation potential based on the assessment of osteogenic markers by real time RT-qPCR, and the evaluation of calcium deposition were also performed. RESULTS: The results showed that diabetes type 2 lowered the activity of ADSCs in proliferation assays and changed their phenotypical characteristics. Interestingly, we observed differences in the proliferation potential of ADSCs in patients with insulin resistance, which is often the first phase of diabetes, compared to the control. It might suggest that insulin resistance, early-stage T2D, alters the activity of cells. Moreover, expression of osteogenesis markers was higher in cells from T2D patients than in cells from patients with IR and control. CONCLUSION: We conclude that type 2 diabetes changes the activity of stem cells, and insulin resistance influences on the proliferation of ADSCs. BioMed Central 2020-07-02 /pmc/articles/PMC7331176/ /pubmed/32615920 http://dx.doi.org/10.1186/s10020-020-00192-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Skubis-Sikora, Aleksandra Sikora, Bartosz Witkowska, Agnieszka Mazurek, Urszula Gola, Joanna Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
title | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
title_full | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
title_fullStr | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
title_full_unstemmed | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
title_short | Osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
title_sort | osteogenesis of adipose-derived stem cells from patients with glucose metabolism disorders |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331176/ https://www.ncbi.nlm.nih.gov/pubmed/32615920 http://dx.doi.org/10.1186/s10020-020-00192-0 |
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