Platelet‐specific antibodies and differences in their expression in childhood immune thrombocytopenic purpura predicts clinical progression

IMPORTANCE: Immune thrombocytopenic purpura (ITP) is the most common bleeding disorder in children. Despite the highly spontaneously remission, still almost 20% of cases progress into chronic or refractory ITP, which seriously affects children's quality of life. Currently there is no method to predict the initial stage of childhood ITP. OBJECTIVES: To evaluate platelet‐specific antibodies and compare differences in their expression in childhood ITP to predict clinical progression. METHODS: This is a single‐center prospective cohort study from April 2014 to October 2015. We enrolled children initially diagnosed as ITP. Anti‐GPIIb/IIIa and GPIb/IX antibodies were assayed by enzyme‐linked immunoadsorbent assay (ELISA) and patients were followed up for 1 year. We also analyzed the relationship between the expression of the platelet‐specific antibodies GPIIb/IIIa and GPIb/IX and their respective clinical prognoses. RESULTS: Overall, 134 cases were enrolled including 77 boys and 57 girls with a median age of 19 months (range: 1 to 159). Positive rates of anti‐platelet antibodies were 79.8%. After a 1‐year observation period, 84.3% were diagnosed as newly diagnosed ITP and 13.4% were diagnosed as chronic ITP. Patients with anti‐GPIIb/IIIa antibody had a higher risk for newly diagnosed ITP compared with patients who were anti‐GPIb/IX antibody positive only (93% vs 25%, P = 0.005; 87% vs 25%, P = 0.014, respectively). There were more anti‐GPIb/IX antibody positive only cases, diagnosed as chronic ITP, compared with anti‐GPIIb/IIIa antibody positive only cases and double GPIIb/IIIa and GPIb/IX antibody positive cases (75% vs 7%, P = 0.005; 75% vs 13%, P = 0.014, respectively). Interpretation INTERPRETATION: Patients with anti‐GPIIb/IIIa antibody (either single or double) were predicted to have a good prognosis, whereas anti‐GPIb/ IX antibody only predicted a poor prognosis. These results should be confirmed via a larger cohort multicenter study.