Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica

The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a were expressed in most organs and could support...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Chunhui, Zhang, Dong, Zheng, Liwei, Shen, Yawen, Zuo, Xiya, Mao, Jiangping, Meng, Yuan, Wu, Haiqin, Zhang, Yike, Liu, Xiaoyuan, Qian, Ming, Zhang, Jie, Li, Gaochao, Zhao, Caiping, Xing, Libo, Ma, Juanjuan, Han, Mingyu, An, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331580/
https://www.ncbi.nlm.nih.gov/pubmed/32616911
http://dx.doi.org/10.1038/s41598-020-66483-y
_version_ 1783553358896824320
author Song, Chunhui
Zhang, Dong
Zheng, Liwei
Shen, Yawen
Zuo, Xiya
Mao, Jiangping
Meng, Yuan
Wu, Haiqin
Zhang, Yike
Liu, Xiaoyuan
Qian, Ming
Zhang, Jie
Li, Gaochao
Zhao, Caiping
Xing, Libo
Ma, Juanjuan
Han, Mingyu
An, Na
author_facet Song, Chunhui
Zhang, Dong
Zheng, Liwei
Shen, Yawen
Zuo, Xiya
Mao, Jiangping
Meng, Yuan
Wu, Haiqin
Zhang, Yike
Liu, Xiaoyuan
Qian, Ming
Zhang, Jie
Li, Gaochao
Zhao, Caiping
Xing, Libo
Ma, Juanjuan
Han, Mingyu
An, Na
author_sort Song, Chunhui
collection PubMed
description The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a were expressed in most organs and could support whole plant basal auxin synthesis. MdYUCCA4a, MdYUCCA10b, and MdYUCCA11a expression indicated roles for these genes in auxin biosynthesis in vegetative organs. MdYUCCA2b, MdYUCCA11b, and MdYUCCA11d were mainly expressed in flower organs. High temperature induced the expression of MdYUCCA4a, MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a, and down-regulated the expression of MdYUCCA2b and MdYUCCA6b. Dual-luciferase assay indicated that MdPIF4 could trans-activate the MdYUCCA8a promoter. Overexpression of MdYUCCA8a increased IAA content, increased stem height, enhanced apical dominance, and led to silique malformation. These results provide a foundation for further investigation of the biological functions of apple MdYUCCAs.
format Online
Article
Text
id pubmed-7331580
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-73315802020-07-06 Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica Song, Chunhui Zhang, Dong Zheng, Liwei Shen, Yawen Zuo, Xiya Mao, Jiangping Meng, Yuan Wu, Haiqin Zhang, Yike Liu, Xiaoyuan Qian, Ming Zhang, Jie Li, Gaochao Zhao, Caiping Xing, Libo Ma, Juanjuan Han, Mingyu An, Na Sci Rep Article The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a were expressed in most organs and could support whole plant basal auxin synthesis. MdYUCCA4a, MdYUCCA10b, and MdYUCCA11a expression indicated roles for these genes in auxin biosynthesis in vegetative organs. MdYUCCA2b, MdYUCCA11b, and MdYUCCA11d were mainly expressed in flower organs. High temperature induced the expression of MdYUCCA4a, MdYUCCA6a, MdYUCCA8a, and MdYUCCA10a, and down-regulated the expression of MdYUCCA2b and MdYUCCA6b. Dual-luciferase assay indicated that MdPIF4 could trans-activate the MdYUCCA8a promoter. Overexpression of MdYUCCA8a increased IAA content, increased stem height, enhanced apical dominance, and led to silique malformation. These results provide a foundation for further investigation of the biological functions of apple MdYUCCAs. Nature Publishing Group UK 2020-07-02 /pmc/articles/PMC7331580/ /pubmed/32616911 http://dx.doi.org/10.1038/s41598-020-66483-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Song, Chunhui
Zhang, Dong
Zheng, Liwei
Shen, Yawen
Zuo, Xiya
Mao, Jiangping
Meng, Yuan
Wu, Haiqin
Zhang, Yike
Liu, Xiaoyuan
Qian, Ming
Zhang, Jie
Li, Gaochao
Zhao, Caiping
Xing, Libo
Ma, Juanjuan
Han, Mingyu
An, Na
Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica
title Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica
title_full Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica
title_fullStr Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica
title_full_unstemmed Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica
title_short Genome-wide identification and expression profiling of the YUCCA gene family in Malus domestica
title_sort genome-wide identification and expression profiling of the yucca gene family in malus domestica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331580/
https://www.ncbi.nlm.nih.gov/pubmed/32616911
http://dx.doi.org/10.1038/s41598-020-66483-y
work_keys_str_mv AT songchunhui genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT zhangdong genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT zhengliwei genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT shenyawen genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT zuoxiya genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT maojiangping genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT mengyuan genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT wuhaiqin genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT zhangyike genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT liuxiaoyuan genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT qianming genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT zhangjie genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT ligaochao genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT zhaocaiping genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT xinglibo genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT majuanjuan genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT hanmingyu genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica
AT anna genomewideidentificationandexpressionprofilingoftheyuccagenefamilyinmalusdomestica

Ejemplares similares