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A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a mult...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331650/ https://www.ncbi.nlm.nih.gov/pubmed/32616824 http://dx.doi.org/10.1038/s41598-020-68072-5 |
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author | Mazzaglia, Angelo Rahi, Yaseen Jundi Taratufolo, Maria Claudia Tatì, Marta Turco, Silvia Ciarroni, Serena Tagliavento, Vincenzo Valentini, Franco D’Onghia, Anna Maria Balestra, Giorgio Mariano |
author_facet | Mazzaglia, Angelo Rahi, Yaseen Jundi Taratufolo, Maria Claudia Tatì, Marta Turco, Silvia Ciarroni, Serena Tagliavento, Vincenzo Valentini, Franco D’Onghia, Anna Maria Balestra, Giorgio Mariano |
author_sort | Mazzaglia, Angelo |
collection | PubMed |
description | The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a multiple locus VNTR analysis assay, a molecular tool to differentiate between populations of the pathogen. It has already been successfully applied to different X. fastidiosa subspecies from various plant hosts. The previously published TR loci, together with a set of new design, have been tested in silico on the genome of the Apulian De Donno strain. The resulting selection of 37 TR loci was amplified on the genomic DNAs of the Apulian strains AND from representatives of X. fastidiosa subspecies, and directly on DNA extracted from infected plants. The assay clearly discerned among subspecies or even sequence types (ST), but also pointed out variants within the same ST so as to provide more detailed information on the dynamics and pathogen diffusion pathways. Its effective application even on total DNAs extracted from infected tissues of different host plants makes it particularly useful for large-scale screening of infection and for the strengthening of containment measures. |
format | Online Article Text |
id | pubmed-7331650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73316502020-07-06 A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy Mazzaglia, Angelo Rahi, Yaseen Jundi Taratufolo, Maria Claudia Tatì, Marta Turco, Silvia Ciarroni, Serena Tagliavento, Vincenzo Valentini, Franco D’Onghia, Anna Maria Balestra, Giorgio Mariano Sci Rep Article The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a multiple locus VNTR analysis assay, a molecular tool to differentiate between populations of the pathogen. It has already been successfully applied to different X. fastidiosa subspecies from various plant hosts. The previously published TR loci, together with a set of new design, have been tested in silico on the genome of the Apulian De Donno strain. The resulting selection of 37 TR loci was amplified on the genomic DNAs of the Apulian strains AND from representatives of X. fastidiosa subspecies, and directly on DNA extracted from infected plants. The assay clearly discerned among subspecies or even sequence types (ST), but also pointed out variants within the same ST so as to provide more detailed information on the dynamics and pathogen diffusion pathways. Its effective application even on total DNAs extracted from infected tissues of different host plants makes it particularly useful for large-scale screening of infection and for the strengthening of containment measures. Nature Publishing Group UK 2020-07-02 /pmc/articles/PMC7331650/ /pubmed/32616824 http://dx.doi.org/10.1038/s41598-020-68072-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mazzaglia, Angelo Rahi, Yaseen Jundi Taratufolo, Maria Claudia Tatì, Marta Turco, Silvia Ciarroni, Serena Tagliavento, Vincenzo Valentini, Franco D’Onghia, Anna Maria Balestra, Giorgio Mariano A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy |
title | A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy |
title_full | A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy |
title_fullStr | A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy |
title_full_unstemmed | A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy |
title_short | A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy |
title_sort | new inclusive mlva assay to investigate genetic variability of xylella fastidiosa with a specific focus on the apulian outbreak in italy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331650/ https://www.ncbi.nlm.nih.gov/pubmed/32616824 http://dx.doi.org/10.1038/s41598-020-68072-5 |
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