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A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy

The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a mult...

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Autores principales: Mazzaglia, Angelo, Rahi, Yaseen Jundi, Taratufolo, Maria Claudia, Tatì, Marta, Turco, Silvia, Ciarroni, Serena, Tagliavento, Vincenzo, Valentini, Franco, D’Onghia, Anna Maria, Balestra, Giorgio Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331650/
https://www.ncbi.nlm.nih.gov/pubmed/32616824
http://dx.doi.org/10.1038/s41598-020-68072-5
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author Mazzaglia, Angelo
Rahi, Yaseen Jundi
Taratufolo, Maria Claudia
Tatì, Marta
Turco, Silvia
Ciarroni, Serena
Tagliavento, Vincenzo
Valentini, Franco
D’Onghia, Anna Maria
Balestra, Giorgio Mariano
author_facet Mazzaglia, Angelo
Rahi, Yaseen Jundi
Taratufolo, Maria Claudia
Tatì, Marta
Turco, Silvia
Ciarroni, Serena
Tagliavento, Vincenzo
Valentini, Franco
D’Onghia, Anna Maria
Balestra, Giorgio Mariano
author_sort Mazzaglia, Angelo
collection PubMed
description The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a multiple locus VNTR analysis assay, a molecular tool to differentiate between populations of the pathogen. It has already been successfully applied to different X. fastidiosa subspecies from various plant hosts. The previously published TR loci, together with a set of new design, have been tested in silico on the genome of the Apulian De Donno strain. The resulting selection of 37 TR loci was amplified on the genomic DNAs of the Apulian strains AND from representatives of X. fastidiosa subspecies, and directly on DNA extracted from infected plants. The assay clearly discerned among subspecies or even sequence types (ST), but also pointed out variants within the same ST so as to provide more detailed information on the dynamics and pathogen diffusion pathways. Its effective application even on total DNAs extracted from infected tissues of different host plants makes it particularly useful for large-scale screening of infection and for the strengthening of containment measures.
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spelling pubmed-73316502020-07-06 A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy Mazzaglia, Angelo Rahi, Yaseen Jundi Taratufolo, Maria Claudia Tatì, Marta Turco, Silvia Ciarroni, Serena Tagliavento, Vincenzo Valentini, Franco D’Onghia, Anna Maria Balestra, Giorgio Mariano Sci Rep Article The Olive Quick Decline Syndrome by Xylella fastidiosa subspecies pauca is among the most severe phytopathological emergencies nowadays. In few years, the outbreak devastated olive groves in Apulia (Italy), potentially endangering the entire Mediterranean basin. This research aimed to develop a multiple locus VNTR analysis assay, a molecular tool to differentiate between populations of the pathogen. It has already been successfully applied to different X. fastidiosa subspecies from various plant hosts. The previously published TR loci, together with a set of new design, have been tested in silico on the genome of the Apulian De Donno strain. The resulting selection of 37 TR loci was amplified on the genomic DNAs of the Apulian strains AND from representatives of X. fastidiosa subspecies, and directly on DNA extracted from infected plants. The assay clearly discerned among subspecies or even sequence types (ST), but also pointed out variants within the same ST so as to provide more detailed information on the dynamics and pathogen diffusion pathways. Its effective application even on total DNAs extracted from infected tissues of different host plants makes it particularly useful for large-scale screening of infection and for the strengthening of containment measures. Nature Publishing Group UK 2020-07-02 /pmc/articles/PMC7331650/ /pubmed/32616824 http://dx.doi.org/10.1038/s41598-020-68072-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mazzaglia, Angelo
Rahi, Yaseen Jundi
Taratufolo, Maria Claudia
Tatì, Marta
Turco, Silvia
Ciarroni, Serena
Tagliavento, Vincenzo
Valentini, Franco
D’Onghia, Anna Maria
Balestra, Giorgio Mariano
A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
title A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
title_full A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
title_fullStr A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
title_full_unstemmed A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
title_short A new inclusive MLVA assay to investigate genetic variability of Xylella fastidiosa with a specific focus on the Apulian outbreak in Italy
title_sort new inclusive mlva assay to investigate genetic variability of xylella fastidiosa with a specific focus on the apulian outbreak in italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331650/
https://www.ncbi.nlm.nih.gov/pubmed/32616824
http://dx.doi.org/10.1038/s41598-020-68072-5
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