A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition

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There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune pro...

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Autores principales: Dieterle, M. Eugenia, Haslwanter, Denise, Bortz, Robert H., Wirchnianski, Ariel S., Lasso, Gorka, Vergnolle, Olivia, Abbasi, Shawn A., Fels, J. Maximilian, Laudermilch, Ethan, Florez, Catalina, Mengotto, Amanda, Kimmel, Duncan, Malonis, Ryan J., Georgiev, George, Quiroz, Jose, Barnhill, Jason, Pirofski, Liise-anne, Daily, Johanna P., Dye, John M., Lai, Jonathan R., Herbert, Andrew S., Chandran, Kartik, Jangra, Rohit K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2020
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332447/
https://www.ncbi.nlm.nih.gov/pubmed/32738193
http://dx.doi.org/10.1016/j.chom.2020.06.020
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author Dieterle, M. Eugenia
Haslwanter, Denise
Bortz, Robert H.
Wirchnianski, Ariel S.
Lasso, Gorka
Vergnolle, Olivia
Abbasi, Shawn A.
Fels, J. Maximilian
Laudermilch, Ethan
Florez, Catalina
Mengotto, Amanda
Kimmel, Duncan
Malonis, Ryan J.
Georgiev, George
Quiroz, Jose
Barnhill, Jason
Pirofski, Liise-anne
Daily, Johanna P.
Dye, John M.
Lai, Jonathan R.
Herbert, Andrew S.
Chandran, Kartik
Jangra, Rohit K.
author_facet Dieterle, M. Eugenia
Haslwanter, Denise
Bortz, Robert H.
Wirchnianski, Ariel S.
Lasso, Gorka
Vergnolle, Olivia
Abbasi, Shawn A.
Fels, J. Maximilian
Laudermilch, Ethan
Florez, Catalina
Mengotto, Amanda
Kimmel, Duncan
Malonis, Ryan J.
Georgiev, George
Quiroz, Jose
Barnhill, Jason
Pirofski, Liise-anne
Daily, Johanna P.
Dye, John M.
Lai, Jonathan R.
Herbert, Andrew S.
Chandran, Kartik
Jangra, Rohit K.
author_sort Dieterle, M. Eugenia
collection PubMed
description There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition.
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spelling pubmed-73324472020-07-06 A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition Dieterle, M. Eugenia Haslwanter, Denise Bortz, Robert H. Wirchnianski, Ariel S. Lasso, Gorka Vergnolle, Olivia Abbasi, Shawn A. Fels, J. Maximilian Laudermilch, Ethan Florez, Catalina Mengotto, Amanda Kimmel, Duncan Malonis, Ryan J. Georgiev, George Quiroz, Jose Barnhill, Jason Pirofski, Liise-anne Daily, Johanna P. Dye, John M. Lai, Jonathan R. Herbert, Andrew S. Chandran, Kartik Jangra, Rohit K. Cell Host Microbe Resource There is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, define correlates of immune protection, and down-select candidate antivirals. Here, we generate a highly infectious recombinant vesicular stomatitis virus (VSV) bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein and show that this recombinant virus, rVSV-SARS-CoV-2 S, closely resembles SARS-CoV-2 in its entry-related properties. The neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in a high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S, and neutralization of rVSV-SARS-CoV-2 S and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific therapeutics and for mechanistic studies of viral entry and its inhibition. Elsevier Inc. 2020-09-09 2020-07-03 /pmc/articles/PMC7332447/ /pubmed/32738193 http://dx.doi.org/10.1016/j.chom.2020.06.020 Text en © 2020 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Resource
Dieterle, M. Eugenia
Haslwanter, Denise
Bortz, Robert H.
Wirchnianski, Ariel S.
Lasso, Gorka
Vergnolle, Olivia
Abbasi, Shawn A.
Fels, J. Maximilian
Laudermilch, Ethan
Florez, Catalina
Mengotto, Amanda
Kimmel, Duncan
Malonis, Ryan J.
Georgiev, George
Quiroz, Jose
Barnhill, Jason
Pirofski, Liise-anne
Daily, Johanna P.
Dye, John M.
Lai, Jonathan R.
Herbert, Andrew S.
Chandran, Kartik
Jangra, Rohit K.
A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition
title A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition
title_full A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition
title_fullStr A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition
title_full_unstemmed A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition
title_short A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition
title_sort replication-competent vesicular stomatitis virus for studies of sars-cov-2 spike-mediated cell entry and its inhibition
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332447/
https://www.ncbi.nlm.nih.gov/pubmed/32738193
http://dx.doi.org/10.1016/j.chom.2020.06.020
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