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Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix

Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report t...

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Autores principales: Telengech, Paul, Hisano, Sakae, Mugambi, Cyrus, Hyodo, Kiwamu, Arjona-López, Juan Manuel, López-Herrera, Carlos José, Kanematsu, Satoko, Kondo, Hideki, Suzuki, Nobuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332551/
https://www.ncbi.nlm.nih.gov/pubmed/32670213
http://dx.doi.org/10.3389/fmicb.2020.01064
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author Telengech, Paul
Hisano, Sakae
Mugambi, Cyrus
Hyodo, Kiwamu
Arjona-López, Juan Manuel
López-Herrera, Carlos José
Kanematsu, Satoko
Kondo, Hideki
Suzuki, Nobuhiro
author_facet Telengech, Paul
Hisano, Sakae
Mugambi, Cyrus
Hyodo, Kiwamu
Arjona-López, Juan Manuel
López-Herrera, Carlos José
Kanematsu, Satoko
Kondo, Hideki
Suzuki, Nobuhiro
author_sort Telengech, Paul
collection PubMed
description Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.
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spelling pubmed-73325512020-07-14 Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix Telengech, Paul Hisano, Sakae Mugambi, Cyrus Hyodo, Kiwamu Arjona-López, Juan Manuel López-Herrera, Carlos José Kanematsu, Satoko Kondo, Hideki Suzuki, Nobuhiro Front Microbiol Microbiology Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed. Frontiers Media S.A. 2020-06-26 /pmc/articles/PMC7332551/ /pubmed/32670213 http://dx.doi.org/10.3389/fmicb.2020.01064 Text en Copyright © 2020 Telengech, Hisano, Mugambi, Hyodo, Arjona-López, López-Herrera, Kanematsu, Kondo and Suzuki. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Telengech, Paul
Hisano, Sakae
Mugambi, Cyrus
Hyodo, Kiwamu
Arjona-López, Juan Manuel
López-Herrera, Carlos José
Kanematsu, Satoko
Kondo, Hideki
Suzuki, Nobuhiro
Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
title Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
title_full Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
title_fullStr Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
title_full_unstemmed Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
title_short Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
title_sort diverse partitiviruses from the phytopathogenic fungus, rosellinia necatrix
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332551/
https://www.ncbi.nlm.nih.gov/pubmed/32670213
http://dx.doi.org/10.3389/fmicb.2020.01064
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