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Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean

GDSL-type esterase/lipase proteins (GELPs) belong to the SGNH hydrolase superfamily and contain a conserved GDSL motif at their N-terminus. GELPs are widely distributed in nature, from microbes to plants, and play crucial roles in growth and development, stress responses and pathogen defense. Howeve...

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Autores principales: Su, Hong-Gang, Zhang, Xiao-Hong, Wang, Ting-Ting, Wei, Wen-Liang, Wang, Yan-Xia, Chen, Jun, Zhou, Yong-Bin, Chen, Ming, Ma, You-Zhi, Xu, Zhao-Shi, Min, Dong-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332888/
https://www.ncbi.nlm.nih.gov/pubmed/32670311
http://dx.doi.org/10.3389/fpls.2020.00726
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author Su, Hong-Gang
Zhang, Xiao-Hong
Wang, Ting-Ting
Wei, Wen-Liang
Wang, Yan-Xia
Chen, Jun
Zhou, Yong-Bin
Chen, Ming
Ma, You-Zhi
Xu, Zhao-Shi
Min, Dong-Hong
author_facet Su, Hong-Gang
Zhang, Xiao-Hong
Wang, Ting-Ting
Wei, Wen-Liang
Wang, Yan-Xia
Chen, Jun
Zhou, Yong-Bin
Chen, Ming
Ma, You-Zhi
Xu, Zhao-Shi
Min, Dong-Hong
author_sort Su, Hong-Gang
collection PubMed
description GDSL-type esterase/lipase proteins (GELPs) belong to the SGNH hydrolase superfamily and contain a conserved GDSL motif at their N-terminus. GELPs are widely distributed in nature, from microbes to plants, and play crucial roles in growth and development, stress responses and pathogen defense. However, the identification and functional analysis of GELP genes are hardly explored in soybean. This study describes the identification of 194 GELP genes in the soybean genome and their phylogenetic classification into 11 subfamilies (A–K). GmGELP genes are disproportionally distributed on 20 soybean chromosomes. Large-scale WGD/segmental duplication events contribute greatly to the expansion of the soybean GDSL gene family. The Ka/Ks ratios of more than 70% of duplicated gene pairs ranged from 0.1–0.3, indicating that most GmGELP genes were under purifying selection pressure. Gene structure analysis indicate that more than 74% of GmGELP genes are interrupted by 4 introns and composed of 5 exons in their coding regions, and closer homologous genes in the phylogenetic tree often have similar exon-intron organization. Further statistics revealed that approximately 56% of subfamily K members contain more than 4 introns, and about 28% of subfamily I members consist of less than 4 introns. For this reason, the two subfamilies were used to simulate intron gain and loss events, respectively. Furthermore, a new model of intron position distribution was established in current study to explore whether the evolution of multi-gene families resulted from the diversity of gene structure. Finally, RNA-seq data were used to investigate the expression profiles of GmGELP gene under different tissues and multiple abiotic stress treatments. Subsequently, 7 stress-responsive GmGELP genes were selected to verify their expression levels by RT-qPCR, the results were consistent with RNA-seq data. Among 7 GmGELP genes, GmGELP28 was selected for further study owing to clear responses to drought, salt and ABA treatments. Transgenic Arabidopsis thaliana and soybean plants showed drought and salt tolerant phenotype. Overexpression of GmGELP28 resulted in the changes of several physiological indicators, which allowed plants to adapt adverse conditions. In all, GmGELP28 is a potential candidate gene for improving the salinity and drought tolerance of soybean.
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spelling pubmed-73328882020-07-14 Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean Su, Hong-Gang Zhang, Xiao-Hong Wang, Ting-Ting Wei, Wen-Liang Wang, Yan-Xia Chen, Jun Zhou, Yong-Bin Chen, Ming Ma, You-Zhi Xu, Zhao-Shi Min, Dong-Hong Front Plant Sci Plant Science GDSL-type esterase/lipase proteins (GELPs) belong to the SGNH hydrolase superfamily and contain a conserved GDSL motif at their N-terminus. GELPs are widely distributed in nature, from microbes to plants, and play crucial roles in growth and development, stress responses and pathogen defense. However, the identification and functional analysis of GELP genes are hardly explored in soybean. This study describes the identification of 194 GELP genes in the soybean genome and their phylogenetic classification into 11 subfamilies (A–K). GmGELP genes are disproportionally distributed on 20 soybean chromosomes. Large-scale WGD/segmental duplication events contribute greatly to the expansion of the soybean GDSL gene family. The Ka/Ks ratios of more than 70% of duplicated gene pairs ranged from 0.1–0.3, indicating that most GmGELP genes were under purifying selection pressure. Gene structure analysis indicate that more than 74% of GmGELP genes are interrupted by 4 introns and composed of 5 exons in their coding regions, and closer homologous genes in the phylogenetic tree often have similar exon-intron organization. Further statistics revealed that approximately 56% of subfamily K members contain more than 4 introns, and about 28% of subfamily I members consist of less than 4 introns. For this reason, the two subfamilies were used to simulate intron gain and loss events, respectively. Furthermore, a new model of intron position distribution was established in current study to explore whether the evolution of multi-gene families resulted from the diversity of gene structure. Finally, RNA-seq data were used to investigate the expression profiles of GmGELP gene under different tissues and multiple abiotic stress treatments. Subsequently, 7 stress-responsive GmGELP genes were selected to verify their expression levels by RT-qPCR, the results were consistent with RNA-seq data. Among 7 GmGELP genes, GmGELP28 was selected for further study owing to clear responses to drought, salt and ABA treatments. Transgenic Arabidopsis thaliana and soybean plants showed drought and salt tolerant phenotype. Overexpression of GmGELP28 resulted in the changes of several physiological indicators, which allowed plants to adapt adverse conditions. In all, GmGELP28 is a potential candidate gene for improving the salinity and drought tolerance of soybean. Frontiers Media S.A. 2020-06-25 /pmc/articles/PMC7332888/ /pubmed/32670311 http://dx.doi.org/10.3389/fpls.2020.00726 Text en Copyright © 2020 Su, Zhang, Wang, Wei, Wang, Chen, Zhou, Chen, Ma, Xu and Min. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Su, Hong-Gang
Zhang, Xiao-Hong
Wang, Ting-Ting
Wei, Wen-Liang
Wang, Yan-Xia
Chen, Jun
Zhou, Yong-Bin
Chen, Ming
Ma, You-Zhi
Xu, Zhao-Shi
Min, Dong-Hong
Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean
title Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean
title_full Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean
title_fullStr Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean
title_full_unstemmed Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean
title_short Genome-Wide Identification, Evolution, and Expression of GDSL-Type Esterase/Lipase Gene Family in Soybean
title_sort genome-wide identification, evolution, and expression of gdsl-type esterase/lipase gene family in soybean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332888/
https://www.ncbi.nlm.nih.gov/pubmed/32670311
http://dx.doi.org/10.3389/fpls.2020.00726
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