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Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide

Lonicera japonica is a traditional Chinese herbal medicine with antioxidation, anti-inflammatory, antibacterial, and immunoregulation functions. A method to isolate polysaccharides from Lonicera japonica (LJP) has been reported previously by our group. We also reported previously that LJP was consis...

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Autores principales: Zhou, Xiaonan, He, Gui, Ma, Jinming, Tang, Min, Tian, Geng, Gong, Xun, Zhang, Huajun, Kui, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333056/
https://www.ncbi.nlm.nih.gov/pubmed/32685499
http://dx.doi.org/10.1155/2020/5279193
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author Zhou, Xiaonan
He, Gui
Ma, Jinming
Tang, Min
Tian, Geng
Gong, Xun
Zhang, Huajun
Kui, Ling
author_facet Zhou, Xiaonan
He, Gui
Ma, Jinming
Tang, Min
Tian, Geng
Gong, Xun
Zhang, Huajun
Kui, Ling
author_sort Zhou, Xiaonan
collection PubMed
description Lonicera japonica is a traditional Chinese herbal medicine with antioxidation, anti-inflammatory, antibacterial, and immunoregulation functions. A method to isolate polysaccharides from Lonicera japonica (LJP) has been reported previously by our group. We also reported previously that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides. In this study, we investigated the protective effect of LJP on cardiomyocytes of mice injured by hydrogen peroxide (H(2)O(2)). The results showed that LJP can increase the cardiomyocyte viability and the activities of the enzyme (SOD, CAT, GSH-Px, AST, CPK, and LDH) in cardiomyocytes of mice injured by hydrogen peroxide. The results of intracellular ROS contents showed that a high dose (40 μg mL(−1)) of LJP had the best effects on protecting the cardiomyocytes of mice injured by H(2)O(2). In addition, the measurement results of the cardiomyocyte apoptosis and the activity of caspase-3, caspase-8, and caspase-9 in cardiomyocytes confirmed this conclusion from another perspective.
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spelling pubmed-73330562020-07-17 Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide Zhou, Xiaonan He, Gui Ma, Jinming Tang, Min Tian, Geng Gong, Xun Zhang, Huajun Kui, Ling Biomed Res Int Research Article Lonicera japonica is a traditional Chinese herbal medicine with antioxidation, anti-inflammatory, antibacterial, and immunoregulation functions. A method to isolate polysaccharides from Lonicera japonica (LJP) has been reported previously by our group. We also reported previously that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides. In this study, we investigated the protective effect of LJP on cardiomyocytes of mice injured by hydrogen peroxide (H(2)O(2)). The results showed that LJP can increase the cardiomyocyte viability and the activities of the enzyme (SOD, CAT, GSH-Px, AST, CPK, and LDH) in cardiomyocytes of mice injured by hydrogen peroxide. The results of intracellular ROS contents showed that a high dose (40 μg mL(−1)) of LJP had the best effects on protecting the cardiomyocytes of mice injured by H(2)O(2). In addition, the measurement results of the cardiomyocyte apoptosis and the activity of caspase-3, caspase-8, and caspase-9 in cardiomyocytes confirmed this conclusion from another perspective. Hindawi 2020-06-23 /pmc/articles/PMC7333056/ /pubmed/32685499 http://dx.doi.org/10.1155/2020/5279193 Text en Copyright © 2020 Xiaonan Zhou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Xiaonan
He, Gui
Ma, Jinming
Tang, Min
Tian, Geng
Gong, Xun
Zhang, Huajun
Kui, Ling
Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide
title Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide
title_full Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide
title_fullStr Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide
title_full_unstemmed Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide
title_short Protective Effect of a Novel Polysaccharide from Lonicera japonica on Cardiomyocytes of Mice Injured by Hydrogen Peroxide
title_sort protective effect of a novel polysaccharide from lonicera japonica on cardiomyocytes of mice injured by hydrogen peroxide
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333056/
https://www.ncbi.nlm.nih.gov/pubmed/32685499
http://dx.doi.org/10.1155/2020/5279193
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