Cargando…

Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation

BACKGROUND: There are many situations of abnormal metabolism influencing liver graft function. This study aims to provide data for the development of liver function recovery after liver transplantation by dynamically analyzing metabolites of bile acids pathway in serum. MATERIAL/METHODS: A comprehen...

Descripción completa

Detalles Bibliográficos
Autores principales: Sui, Weiguo, Gan, Qing, Liu, Fuhua, Ou, Minglin, Wang, Bingguo, Liao, Songbai, Lai, Liusheng, Chen, Huaizhou, Yang, Ming, Dai, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333510/
https://www.ncbi.nlm.nih.gov/pubmed/32572018
http://dx.doi.org/10.12659/AOT.921844
_version_ 1783553769151135744
author Sui, Weiguo
Gan, Qing
Liu, Fuhua
Ou, Minglin
Wang, Bingguo
Liao, Songbai
Lai, Liusheng
Chen, Huaizhou
Yang, Ming
Dai, Yong
author_facet Sui, Weiguo
Gan, Qing
Liu, Fuhua
Ou, Minglin
Wang, Bingguo
Liao, Songbai
Lai, Liusheng
Chen, Huaizhou
Yang, Ming
Dai, Yong
author_sort Sui, Weiguo
collection PubMed
description BACKGROUND: There are many situations of abnormal metabolism influencing liver graft function. This study aims to provide data for the development of liver function recovery after liver transplantation by dynamically analyzing metabolites of bile acids pathway in serum. MATERIAL/METHODS: A comprehensive metabolomics profiling of serum of 9 liver transplantation patients before transplantation, on the 1(st), 3(rd), and 7(th) days after liver transplantation, and healthy individuals were performed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Multivariate data and dynamic analysis were used to search for biomarkers between the metabolomics profiles present in perioperative liver transplantation and normal controls. RESULTS: Thirty-three differential endogenous metabolites were screened by the threshold of variable importance in the projection (VIP) from an orthogonal partial least square discriminant analysis (OPLS-DA) greater than 1.0, q-value <0.05, and fold change (FC) ≤0.8 or ≥1.2 between the preoperative group and the normal controls in negative mode. The metabolite intensities of taurocholic acid, taurochenodeoxycholic acid, chenodeoxycholic acid glycine conjugate, and glycocholic acid pre-transplantation were significantly higher than those of normal controls. The average metabolite intensities of taurocholic acid and taurochenodesoxycholic acid on the first day after liver transplantation were lower than those observed pre-transplantation. The average metabolite intensities on day 3 after liver transplantation showed a sudden increase and then decreased after 7 postoperative days. The average metabolite intensities of glycocholic acid and chenodeoxycholic acid glycine conjugate showed an increasing trend on the 1(st), 3(rd), and 7(th) days after liver transplantation. CONCLUSIONS: Use of taurocholic acid and taurochenodeoxycholic acid-related bile secretion, liver regeneration, and de novo bile acid synthesis may help clinical evaluation and provide data for the development of liver function recovery after liver transplantation.
format Online
Article
Text
id pubmed-7333510
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-73335102020-07-08 Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation Sui, Weiguo Gan, Qing Liu, Fuhua Ou, Minglin Wang, Bingguo Liao, Songbai Lai, Liusheng Chen, Huaizhou Yang, Ming Dai, Yong Ann Transplant Original Paper BACKGROUND: There are many situations of abnormal metabolism influencing liver graft function. This study aims to provide data for the development of liver function recovery after liver transplantation by dynamically analyzing metabolites of bile acids pathway in serum. MATERIAL/METHODS: A comprehensive metabolomics profiling of serum of 9 liver transplantation patients before transplantation, on the 1(st), 3(rd), and 7(th) days after liver transplantation, and healthy individuals were performed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Multivariate data and dynamic analysis were used to search for biomarkers between the metabolomics profiles present in perioperative liver transplantation and normal controls. RESULTS: Thirty-three differential endogenous metabolites were screened by the threshold of variable importance in the projection (VIP) from an orthogonal partial least square discriminant analysis (OPLS-DA) greater than 1.0, q-value <0.05, and fold change (FC) ≤0.8 or ≥1.2 between the preoperative group and the normal controls in negative mode. The metabolite intensities of taurocholic acid, taurochenodeoxycholic acid, chenodeoxycholic acid glycine conjugate, and glycocholic acid pre-transplantation were significantly higher than those of normal controls. The average metabolite intensities of taurocholic acid and taurochenodesoxycholic acid on the first day after liver transplantation were lower than those observed pre-transplantation. The average metabolite intensities on day 3 after liver transplantation showed a sudden increase and then decreased after 7 postoperative days. The average metabolite intensities of glycocholic acid and chenodeoxycholic acid glycine conjugate showed an increasing trend on the 1(st), 3(rd), and 7(th) days after liver transplantation. CONCLUSIONS: Use of taurocholic acid and taurochenodeoxycholic acid-related bile secretion, liver regeneration, and de novo bile acid synthesis may help clinical evaluation and provide data for the development of liver function recovery after liver transplantation. International Scientific Literature, Inc. 2020-06-23 /pmc/articles/PMC7333510/ /pubmed/32572018 http://dx.doi.org/10.12659/AOT.921844 Text en © Ann Transplant, 2020 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Original Paper
Sui, Weiguo
Gan, Qing
Liu, Fuhua
Ou, Minglin
Wang, Bingguo
Liao, Songbai
Lai, Liusheng
Chen, Huaizhou
Yang, Ming
Dai, Yong
Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
title Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
title_full Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
title_fullStr Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
title_full_unstemmed Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
title_short Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
title_sort dynamic metabolomics study of the bile acid pathway during perioperative primary hepatic carcinoma following liver transplantation
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333510/
https://www.ncbi.nlm.nih.gov/pubmed/32572018
http://dx.doi.org/10.12659/AOT.921844
work_keys_str_mv AT suiweiguo dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT ganqing dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT liufuhua dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT ouminglin dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT wangbingguo dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT liaosongbai dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT lailiusheng dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT chenhuaizhou dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT yangming dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation
AT daiyong dynamicmetabolomicsstudyofthebileacidpathwayduringperioperativeprimaryhepaticcarcinomafollowinglivertransplantation