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Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation
BACKGROUND: There are many situations of abnormal metabolism influencing liver graft function. This study aims to provide data for the development of liver function recovery after liver transplantation by dynamically analyzing metabolites of bile acids pathway in serum. MATERIAL/METHODS: A comprehen...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333510/ https://www.ncbi.nlm.nih.gov/pubmed/32572018 http://dx.doi.org/10.12659/AOT.921844 |
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author | Sui, Weiguo Gan, Qing Liu, Fuhua Ou, Minglin Wang, Bingguo Liao, Songbai Lai, Liusheng Chen, Huaizhou Yang, Ming Dai, Yong |
author_facet | Sui, Weiguo Gan, Qing Liu, Fuhua Ou, Minglin Wang, Bingguo Liao, Songbai Lai, Liusheng Chen, Huaizhou Yang, Ming Dai, Yong |
author_sort | Sui, Weiguo |
collection | PubMed |
description | BACKGROUND: There are many situations of abnormal metabolism influencing liver graft function. This study aims to provide data for the development of liver function recovery after liver transplantation by dynamically analyzing metabolites of bile acids pathway in serum. MATERIAL/METHODS: A comprehensive metabolomics profiling of serum of 9 liver transplantation patients before transplantation, on the 1(st), 3(rd), and 7(th) days after liver transplantation, and healthy individuals were performed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Multivariate data and dynamic analysis were used to search for biomarkers between the metabolomics profiles present in perioperative liver transplantation and normal controls. RESULTS: Thirty-three differential endogenous metabolites were screened by the threshold of variable importance in the projection (VIP) from an orthogonal partial least square discriminant analysis (OPLS-DA) greater than 1.0, q-value <0.05, and fold change (FC) ≤0.8 or ≥1.2 between the preoperative group and the normal controls in negative mode. The metabolite intensities of taurocholic acid, taurochenodeoxycholic acid, chenodeoxycholic acid glycine conjugate, and glycocholic acid pre-transplantation were significantly higher than those of normal controls. The average metabolite intensities of taurocholic acid and taurochenodesoxycholic acid on the first day after liver transplantation were lower than those observed pre-transplantation. The average metabolite intensities on day 3 after liver transplantation showed a sudden increase and then decreased after 7 postoperative days. The average metabolite intensities of glycocholic acid and chenodeoxycholic acid glycine conjugate showed an increasing trend on the 1(st), 3(rd), and 7(th) days after liver transplantation. CONCLUSIONS: Use of taurocholic acid and taurochenodeoxycholic acid-related bile secretion, liver regeneration, and de novo bile acid synthesis may help clinical evaluation and provide data for the development of liver function recovery after liver transplantation. |
format | Online Article Text |
id | pubmed-7333510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73335102020-07-08 Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation Sui, Weiguo Gan, Qing Liu, Fuhua Ou, Minglin Wang, Bingguo Liao, Songbai Lai, Liusheng Chen, Huaizhou Yang, Ming Dai, Yong Ann Transplant Original Paper BACKGROUND: There are many situations of abnormal metabolism influencing liver graft function. This study aims to provide data for the development of liver function recovery after liver transplantation by dynamically analyzing metabolites of bile acids pathway in serum. MATERIAL/METHODS: A comprehensive metabolomics profiling of serum of 9 liver transplantation patients before transplantation, on the 1(st), 3(rd), and 7(th) days after liver transplantation, and healthy individuals were performed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Multivariate data and dynamic analysis were used to search for biomarkers between the metabolomics profiles present in perioperative liver transplantation and normal controls. RESULTS: Thirty-three differential endogenous metabolites were screened by the threshold of variable importance in the projection (VIP) from an orthogonal partial least square discriminant analysis (OPLS-DA) greater than 1.0, q-value <0.05, and fold change (FC) ≤0.8 or ≥1.2 between the preoperative group and the normal controls in negative mode. The metabolite intensities of taurocholic acid, taurochenodeoxycholic acid, chenodeoxycholic acid glycine conjugate, and glycocholic acid pre-transplantation were significantly higher than those of normal controls. The average metabolite intensities of taurocholic acid and taurochenodesoxycholic acid on the first day after liver transplantation were lower than those observed pre-transplantation. The average metabolite intensities on day 3 after liver transplantation showed a sudden increase and then decreased after 7 postoperative days. The average metabolite intensities of glycocholic acid and chenodeoxycholic acid glycine conjugate showed an increasing trend on the 1(st), 3(rd), and 7(th) days after liver transplantation. CONCLUSIONS: Use of taurocholic acid and taurochenodeoxycholic acid-related bile secretion, liver regeneration, and de novo bile acid synthesis may help clinical evaluation and provide data for the development of liver function recovery after liver transplantation. International Scientific Literature, Inc. 2020-06-23 /pmc/articles/PMC7333510/ /pubmed/32572018 http://dx.doi.org/10.12659/AOT.921844 Text en © Ann Transplant, 2020 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Original Paper Sui, Weiguo Gan, Qing Liu, Fuhua Ou, Minglin Wang, Bingguo Liao, Songbai Lai, Liusheng Chen, Huaizhou Yang, Ming Dai, Yong Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation |
title | Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation |
title_full | Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation |
title_fullStr | Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation |
title_full_unstemmed | Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation |
title_short | Dynamic Metabolomics Study of the Bile Acid Pathway During Perioperative Primary Hepatic Carcinoma Following Liver Transplantation |
title_sort | dynamic metabolomics study of the bile acid pathway during perioperative primary hepatic carcinoma following liver transplantation |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333510/ https://www.ncbi.nlm.nih.gov/pubmed/32572018 http://dx.doi.org/10.12659/AOT.921844 |
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