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Optimization of western blotting for the detection of proteins of different molecular weight

Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended...

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Detalles Bibliográficos
Autores principales:	Heda, Ghanshyam D, Shrestha, Lisa, Thapa, Sagarina, Ghimire, Shreya, Raut, Diptika
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Future Science Ltd 2020
Materias:	Reports
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333534/ https://www.ncbi.nlm.nih.gov/pubmed/32283940 http://dx.doi.org/10.2144/btn-2019-0124

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