FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis

Lipid metabolism participates in regulating the functions of granulosa cells (GCs), which is important for follicular development. In this experiment, goose GCs from pre-hierarchical follicles and hierarchical follicles were selected to be the model for studying the putative regulatory role of lipid...

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Autores principales: Chen, Xi, Huang, Kailiang, Hu, Shenqiang, Lan, Gang, Gan, Xiang, Gao, Shanyan, Deng, Yan, Hu, Jiwei, Li, Liang, Hu, Bo, He, Hua, Liu, Hehe, Xia, Lu, Wang, Jiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333536/
https://www.ncbi.nlm.nih.gov/pubmed/32676035
http://dx.doi.org/10.3389/fphys.2020.00600
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author Chen, Xi
Huang, Kailiang
Hu, Shenqiang
Lan, Gang
Gan, Xiang
Gao, Shanyan
Deng, Yan
Hu, Jiwei
Li, Liang
Hu, Bo
He, Hua
Liu, Hehe
Xia, Lu
Wang, Jiwen
author_facet Chen, Xi
Huang, Kailiang
Hu, Shenqiang
Lan, Gang
Gan, Xiang
Gao, Shanyan
Deng, Yan
Hu, Jiwei
Li, Liang
Hu, Bo
He, Hua
Liu, Hehe
Xia, Lu
Wang, Jiwen
author_sort Chen, Xi
collection PubMed
description Lipid metabolism participates in regulating the functions of granulosa cells (GCs), which is important for follicular development. In this experiment, goose GCs from pre-hierarchical follicles and hierarchical follicles were selected to be the model for studying the putative regulatory role of lipid metabolism in apoptosis and steroidogenesis, through overexpression and interference with fatty acid synthase (FASN). When FASN was overexpressed, the lipid accumulation was increased in hierarchical GCs (hGCs) and it was increased in the two categorized GCs when FASN was interfered. In addition, the apoptosis of the two categorized GCs was increased when FASN was overexpressed, and their progesterone production was decreased when FASN was interfered. The results of qRT-PCR showed that, when FASN was overexpressed, the expression level of CYP11A1 was decreased in pre-hierarchical GCs (phGCs), while the expression levels of SCD1, DGAT2, APOB, and StAR were increased in hGCs. When FASN was interfered, the expression levels of CPT-1, DGAT2, and StAR were decreased whereas the expression level of CYP11A1 was increased in phGCs, and the expression levels of CPT-1, SCD1, and StAR were decreased in hGCs. These results not only identify the different effects of manipulated FASN expression on lipid metabolism of goose phGCs and hGCs but also demonstrate that FASN-mediated lipid metabolism plays an important role in regulating apoptosis and steroidogenesis of in vitro cultured goose GCs.
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spelling pubmed-73335362020-07-15 FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis Chen, Xi Huang, Kailiang Hu, Shenqiang Lan, Gang Gan, Xiang Gao, Shanyan Deng, Yan Hu, Jiwei Li, Liang Hu, Bo He, Hua Liu, Hehe Xia, Lu Wang, Jiwen Front Physiol Physiology Lipid metabolism participates in regulating the functions of granulosa cells (GCs), which is important for follicular development. In this experiment, goose GCs from pre-hierarchical follicles and hierarchical follicles were selected to be the model for studying the putative regulatory role of lipid metabolism in apoptosis and steroidogenesis, through overexpression and interference with fatty acid synthase (FASN). When FASN was overexpressed, the lipid accumulation was increased in hierarchical GCs (hGCs) and it was increased in the two categorized GCs when FASN was interfered. In addition, the apoptosis of the two categorized GCs was increased when FASN was overexpressed, and their progesterone production was decreased when FASN was interfered. The results of qRT-PCR showed that, when FASN was overexpressed, the expression level of CYP11A1 was decreased in pre-hierarchical GCs (phGCs), while the expression levels of SCD1, DGAT2, APOB, and StAR were increased in hGCs. When FASN was interfered, the expression levels of CPT-1, DGAT2, and StAR were decreased whereas the expression level of CYP11A1 was increased in phGCs, and the expression levels of CPT-1, SCD1, and StAR were decreased in hGCs. These results not only identify the different effects of manipulated FASN expression on lipid metabolism of goose phGCs and hGCs but also demonstrate that FASN-mediated lipid metabolism plays an important role in regulating apoptosis and steroidogenesis of in vitro cultured goose GCs. Frontiers Media S.A. 2020-06-26 /pmc/articles/PMC7333536/ /pubmed/32676035 http://dx.doi.org/10.3389/fphys.2020.00600 Text en Copyright © 2020 Chen, Huang, Hu, Lan, Gan, Gao, Deng, Hu, Li, Hu, He, Liu, Xia and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chen, Xi
Huang, Kailiang
Hu, Shenqiang
Lan, Gang
Gan, Xiang
Gao, Shanyan
Deng, Yan
Hu, Jiwei
Li, Liang
Hu, Bo
He, Hua
Liu, Hehe
Xia, Lu
Wang, Jiwen
FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis
title FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis
title_full FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis
title_fullStr FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis
title_full_unstemmed FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis
title_short FASN-Mediated Lipid Metabolism Regulates Goose Granulosa Cells Apoptosis and Steroidogenesis
title_sort fasn-mediated lipid metabolism regulates goose granulosa cells apoptosis and steroidogenesis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333536/
https://www.ncbi.nlm.nih.gov/pubmed/32676035
http://dx.doi.org/10.3389/fphys.2020.00600
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