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Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations
The medial amygdala (MeA) is essential for processing innate social and non-social behaviors, such as territorial aggression and mating, which display in a sex-specific manner. While sex differences in cell numbers and neuronal morphology in the MeA are well established, if and how these differences...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333980/ https://www.ncbi.nlm.nih.gov/pubmed/32493755 http://dx.doi.org/10.1523/ENEURO.0035-20.2020 |
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author | Matos, Heidi Y. Hernandez-Pineda, David Charpentier, Claire M. Rusk, Allison Corbin, Joshua G. Jones, Kevin S. |
author_facet | Matos, Heidi Y. Hernandez-Pineda, David Charpentier, Claire M. Rusk, Allison Corbin, Joshua G. Jones, Kevin S. |
author_sort | Matos, Heidi Y. |
collection | PubMed |
description | The medial amygdala (MeA) is essential for processing innate social and non-social behaviors, such as territorial aggression and mating, which display in a sex-specific manner. While sex differences in cell numbers and neuronal morphology in the MeA are well established, if and how these differences extend to the biophysical level remain unknown. Our previous studies revealed that expression of the transcription factors, Dbx1 and Foxp2, during embryogenesis defines separate progenitor pools destined to generate different subclasses of MEA inhibitory output neurons. We have also previously shown that Dbx1-lineage and Foxp2-lineage neurons display different responses to innate olfactory cues and in a sex-specific manner. To examine whether these neurons also possess sex-specific biophysical signatures, we conducted a multidimensional analysis of the intrinsic electrophysiological profiles of these transcription factor defined neurons in the male and female MeA. We observed striking differences in the action potential (AP) spiking patterns across lineages, and across sex within each lineage, properties known to be modified by different voltage-gated ion channels. To identify the potential mechanism underlying the observed lineage-specific and sex-specific differences in spiking adaptation, we conducted a phase plot analysis to narrow down putative ion channel candidates. Of these candidates, we found a subset expressed in a lineage-biased and/or sex-biased manner. Thus, our results uncover neuronal subpopulation and sex differences in the biophysical signatures of developmentally defined MeA output neurons, providing a potential physiological substrate for how the male and female MeA may process social and non-social cues that trigger innate behavioral responses. |
format | Online Article Text |
id | pubmed-7333980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-73339802020-07-06 Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations Matos, Heidi Y. Hernandez-Pineda, David Charpentier, Claire M. Rusk, Allison Corbin, Joshua G. Jones, Kevin S. eNeuro Research Article: New Research The medial amygdala (MeA) is essential for processing innate social and non-social behaviors, such as territorial aggression and mating, which display in a sex-specific manner. While sex differences in cell numbers and neuronal morphology in the MeA are well established, if and how these differences extend to the biophysical level remain unknown. Our previous studies revealed that expression of the transcription factors, Dbx1 and Foxp2, during embryogenesis defines separate progenitor pools destined to generate different subclasses of MEA inhibitory output neurons. We have also previously shown that Dbx1-lineage and Foxp2-lineage neurons display different responses to innate olfactory cues and in a sex-specific manner. To examine whether these neurons also possess sex-specific biophysical signatures, we conducted a multidimensional analysis of the intrinsic electrophysiological profiles of these transcription factor defined neurons in the male and female MeA. We observed striking differences in the action potential (AP) spiking patterns across lineages, and across sex within each lineage, properties known to be modified by different voltage-gated ion channels. To identify the potential mechanism underlying the observed lineage-specific and sex-specific differences in spiking adaptation, we conducted a phase plot analysis to narrow down putative ion channel candidates. Of these candidates, we found a subset expressed in a lineage-biased and/or sex-biased manner. Thus, our results uncover neuronal subpopulation and sex differences in the biophysical signatures of developmentally defined MeA output neurons, providing a potential physiological substrate for how the male and female MeA may process social and non-social cues that trigger innate behavioral responses. Society for Neuroscience 2020-07-02 /pmc/articles/PMC7333980/ /pubmed/32493755 http://dx.doi.org/10.1523/ENEURO.0035-20.2020 Text en Copyright © 2020 Matos et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article: New Research Matos, Heidi Y. Hernandez-Pineda, David Charpentier, Claire M. Rusk, Allison Corbin, Joshua G. Jones, Kevin S. Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations |
title | Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations |
title_full | Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations |
title_fullStr | Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations |
title_full_unstemmed | Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations |
title_short | Sex Differences in Biophysical Signatures across Molecularly Defined Medial Amygdala Neuronal Subpopulations |
title_sort | sex differences in biophysical signatures across molecularly defined medial amygdala neuronal subpopulations |
topic | Research Article: New Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333980/ https://www.ncbi.nlm.nih.gov/pubmed/32493755 http://dx.doi.org/10.1523/ENEURO.0035-20.2020 |
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