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Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress

Primary cilia of tubular cells are sensory organelles. Bending of the primary cilia with shear stress from urinary flow results in the elevation of intracellular calcium levels and activation of signaling pathways that maintain kidney function. Elongation of primary cilia is reported to occur due to...

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Autores principales: Chikamori, Masatomo, Kimura, Hiroshi, Inagi, Reiko, Zhou, Jing, Nangaku, Masaomi, Fujii, Teruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334031/
https://www.ncbi.nlm.nih.gov/pubmed/32665806
http://dx.doi.org/10.1063/5.0010737
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author Chikamori, Masatomo
Kimura, Hiroshi
Inagi, Reiko
Zhou, Jing
Nangaku, Masaomi
Fujii, Teruo
author_facet Chikamori, Masatomo
Kimura, Hiroshi
Inagi, Reiko
Zhou, Jing
Nangaku, Masaomi
Fujii, Teruo
author_sort Chikamori, Masatomo
collection PubMed
description Primary cilia of tubular cells are sensory organelles. Bending of the primary cilia with shear stress from urinary flow results in the elevation of intracellular calcium levels and activation of signaling pathways that maintain kidney function. Elongation of primary cilia is reported to occur due to oxidative stress, which is a major cause of ischemia-reperfusion injury and is accompanied by decreased kidney function. However, in the context of diminished kidney function, this elongation is yet to be investigated. In this study, we developed a new microfluidic device to monitor changes in the intracellular calcium levels while modulating shear stress on the cilia under different degrees of oxidative stress. The microfluidic device was designed to expose even shear stress in the observed area while supplying drugs in four different stepwise concentrations. The results showed that primary cilia were elongated by hydrogen peroxide, which induces oxidative stress. It was also observed that the elongated primary cilia were more sensitive to shear stress than those with normal morphology. This microfluidic device could, thus, be useful in the analysis of the morphology of the primary cilia, under low perfusion conditions.
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spelling pubmed-73340312020-07-13 Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress Chikamori, Masatomo Kimura, Hiroshi Inagi, Reiko Zhou, Jing Nangaku, Masaomi Fujii, Teruo Biomicrofluidics Regular Articles Primary cilia of tubular cells are sensory organelles. Bending of the primary cilia with shear stress from urinary flow results in the elevation of intracellular calcium levels and activation of signaling pathways that maintain kidney function. Elongation of primary cilia is reported to occur due to oxidative stress, which is a major cause of ischemia-reperfusion injury and is accompanied by decreased kidney function. However, in the context of diminished kidney function, this elongation is yet to be investigated. In this study, we developed a new microfluidic device to monitor changes in the intracellular calcium levels while modulating shear stress on the cilia under different degrees of oxidative stress. The microfluidic device was designed to expose even shear stress in the observed area while supplying drugs in four different stepwise concentrations. The results showed that primary cilia were elongated by hydrogen peroxide, which induces oxidative stress. It was also observed that the elongated primary cilia were more sensitive to shear stress than those with normal morphology. This microfluidic device could, thus, be useful in the analysis of the morphology of the primary cilia, under low perfusion conditions. AIP Publishing LLC 2020-07-02 /pmc/articles/PMC7334031/ /pubmed/32665806 http://dx.doi.org/10.1063/5.0010737 Text en © 2020 Author(s). 1932-1058/2020/14(4)/044102/6 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Articles
Chikamori, Masatomo
Kimura, Hiroshi
Inagi, Reiko
Zhou, Jing
Nangaku, Masaomi
Fujii, Teruo
Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
title Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
title_full Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
title_fullStr Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
title_full_unstemmed Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
title_short Intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
title_sort intracellular calcium response of primary cilia of tubular cells to modulated shear stress under oxidative stress
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334031/
https://www.ncbi.nlm.nih.gov/pubmed/32665806
http://dx.doi.org/10.1063/5.0010737
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