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Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans
Variability in gene expression across a population of homogeneous cells is known to influence various biological processes. In model organisms, natural genetic variants were found that modify expression dispersion (variability at a fixed mean) but very few studies have detected such effects in human...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335051/ https://www.ncbi.nlm.nih.gov/pubmed/32620900 http://dx.doi.org/10.1038/s42003-020-1075-1 |
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author | Triqueneaux, Gérard Burny, Claire Symmons, Orsolya Janczarski, Stéphane Gruffat, Henri Yvert, Gaël |
author_facet | Triqueneaux, Gérard Burny, Claire Symmons, Orsolya Janczarski, Stéphane Gruffat, Henri Yvert, Gaël |
author_sort | Triqueneaux, Gérard |
collection | PubMed |
description | Variability in gene expression across a population of homogeneous cells is known to influence various biological processes. In model organisms, natural genetic variants were found that modify expression dispersion (variability at a fixed mean) but very few studies have detected such effects in humans. Here, we analyzed single-cell expression of four proteins (CD23, CD55, CD63 and CD86) across cell lines derived from individuals of the Yoruba population. Using data from over 30 million cells, we found substantial inter-individual variation of dispersion. We demonstrate, via de novo cell line generation and subcloning experiments, that this variation exceeds the variation associated with cellular immortalization. We detected a genetic association between the expression dispersion of CD63 and the rs971 SNP. Our results show that human DNA variants can have inherently-probabilistic effects on gene expression. Such subtle genetic effects may participate to phenotypic variation and disease outcome. |
format | Online Article Text |
id | pubmed-7335051 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-73350512020-07-09 Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans Triqueneaux, Gérard Burny, Claire Symmons, Orsolya Janczarski, Stéphane Gruffat, Henri Yvert, Gaël Commun Biol Article Variability in gene expression across a population of homogeneous cells is known to influence various biological processes. In model organisms, natural genetic variants were found that modify expression dispersion (variability at a fixed mean) but very few studies have detected such effects in humans. Here, we analyzed single-cell expression of four proteins (CD23, CD55, CD63 and CD86) across cell lines derived from individuals of the Yoruba population. Using data from over 30 million cells, we found substantial inter-individual variation of dispersion. We demonstrate, via de novo cell line generation and subcloning experiments, that this variation exceeds the variation associated with cellular immortalization. We detected a genetic association between the expression dispersion of CD63 and the rs971 SNP. Our results show that human DNA variants can have inherently-probabilistic effects on gene expression. Such subtle genetic effects may participate to phenotypic variation and disease outcome. Nature Publishing Group UK 2020-07-03 /pmc/articles/PMC7335051/ /pubmed/32620900 http://dx.doi.org/10.1038/s42003-020-1075-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Triqueneaux, Gérard Burny, Claire Symmons, Orsolya Janczarski, Stéphane Gruffat, Henri Yvert, Gaël Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans |
title | Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans |
title_full | Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans |
title_fullStr | Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans |
title_full_unstemmed | Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans |
title_short | Cell-to-cell expression dispersion of B-cell surface proteins is linked to genetic variants in humans |
title_sort | cell-to-cell expression dispersion of b-cell surface proteins is linked to genetic variants in humans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335051/ https://www.ncbi.nlm.nih.gov/pubmed/32620900 http://dx.doi.org/10.1038/s42003-020-1075-1 |
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