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Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells

Proteomic analysis identifies post-translational functions of proteins, which remains obscure in transcriptomics. Given the important functions of Th9 cells in anti-tumor immunity, we performed proteome analysis of Th9 cells to understand the involvement of proteins that might be crucial for the ant...

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Autores principales: Roy, Suyasha, Goel, Renu, Aggarwal, Suruchi, Asthana, Shailendra, Yadav, Amit Kumar, Awasthi, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335106/
https://www.ncbi.nlm.nih.gov/pubmed/32620893
http://dx.doi.org/10.1038/s41598-020-67845-2
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author Roy, Suyasha
Goel, Renu
Aggarwal, Suruchi
Asthana, Shailendra
Yadav, Amit Kumar
Awasthi, Amit
author_facet Roy, Suyasha
Goel, Renu
Aggarwal, Suruchi
Asthana, Shailendra
Yadav, Amit Kumar
Awasthi, Amit
author_sort Roy, Suyasha
collection PubMed
description Proteomic analysis identifies post-translational functions of proteins, which remains obscure in transcriptomics. Given the important functions of Th9 cells in anti-tumor immunity, we performed proteome analysis of Th9 cells to understand the involvement of proteins that might be crucial for the anti-tumor functions of Th9 cells. Here we performed a comprehensive proteomic analysis of murine Th0 and Th9 cells, and identified proteins that are enriched in Th9 cells. Pathway analysis identified an abundance of phosphoproteins in the proteome of Th9 cells as compared to Th0 cells. Among upregulated phosphoproteins, Ppp2ca (catalytic subunit of protein phosphatase, PP2A) was found to be highly enriched in Th9 cells. Although the role of PP2A has been shown to regulate the differentiation and functions of Th1, Th2, Th17 and Tregs, its role in the differentiation and functions of Th9 cells is not identified yet. Here we found that PP2A is required for the induction of Th9 cells, as PP2A inhibition leads to the suppression of IL-9 and expression of key transcription factors of Th9 cells. PP2A inhibition abrogates Th9 cell-mediated anti-tumor immune response in B16-OVA melanoma tumor model. Thus, we report that PP2A is essential for the differentiation and anti-tumor functions of Th9 cells.
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spelling pubmed-73351062020-07-07 Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells Roy, Suyasha Goel, Renu Aggarwal, Suruchi Asthana, Shailendra Yadav, Amit Kumar Awasthi, Amit Sci Rep Article Proteomic analysis identifies post-translational functions of proteins, which remains obscure in transcriptomics. Given the important functions of Th9 cells in anti-tumor immunity, we performed proteome analysis of Th9 cells to understand the involvement of proteins that might be crucial for the anti-tumor functions of Th9 cells. Here we performed a comprehensive proteomic analysis of murine Th0 and Th9 cells, and identified proteins that are enriched in Th9 cells. Pathway analysis identified an abundance of phosphoproteins in the proteome of Th9 cells as compared to Th0 cells. Among upregulated phosphoproteins, Ppp2ca (catalytic subunit of protein phosphatase, PP2A) was found to be highly enriched in Th9 cells. Although the role of PP2A has been shown to regulate the differentiation and functions of Th1, Th2, Th17 and Tregs, its role in the differentiation and functions of Th9 cells is not identified yet. Here we found that PP2A is required for the induction of Th9 cells, as PP2A inhibition leads to the suppression of IL-9 and expression of key transcription factors of Th9 cells. PP2A inhibition abrogates Th9 cell-mediated anti-tumor immune response in B16-OVA melanoma tumor model. Thus, we report that PP2A is essential for the differentiation and anti-tumor functions of Th9 cells. Nature Publishing Group UK 2020-07-03 /pmc/articles/PMC7335106/ /pubmed/32620893 http://dx.doi.org/10.1038/s41598-020-67845-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Roy, Suyasha
Goel, Renu
Aggarwal, Suruchi
Asthana, Shailendra
Yadav, Amit Kumar
Awasthi, Amit
Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
title Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
title_full Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
title_fullStr Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
title_full_unstemmed Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
title_short Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cells
title_sort proteome analysis revealed the essential functions of protein phosphatase pp2a in the induction of th9 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335106/
https://www.ncbi.nlm.nih.gov/pubmed/32620893
http://dx.doi.org/10.1038/s41598-020-67845-2
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