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Fast three-color single-molecule FRET using statistical inference

We describe theory, experiments, and analyses of three-color Förster resonance energy transfer (FRET) spectroscopy for probing sub-millisecond conformational dynamics of protein folding and binding of disordered proteins. We devise a scheme that uses single continuous-wave laser excitation of the do...

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Detalles Bibliográficos
Autores principales: Yoo, Janghyun, Kim, Jae-Yeol, Louis, John M., Gopich, Irina V., Chung, Hoi Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335206/
https://www.ncbi.nlm.nih.gov/pubmed/32620782
http://dx.doi.org/10.1038/s41467-020-17149-w