jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Femtosecond lasers at fixed wavelengths above 1000 nm are powerful, stable, and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a...

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Detalles Bibliográficos
Autores principales: Mohr, Manuel Alexander, Bushey, Daniel, Aggarwal, Abhi, Marvin, Jonathan S., Kim, Jeong Jun, Marquez, Emiliano Jimenez, Liang, Yajie, Patel, Ronak, Macklin, John J., Lee, Chi-Yu, Tsang, Arthur, Tsegaye, Getahun, Ahrens, Allison M., Chen, Jerry L., Kim, Douglas S., Wong, Allan M., Looger, Loren L., Schreiter, Eric R., Podgorski, Kaspar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335340/
https://www.ncbi.nlm.nih.gov/pubmed/32451475
http://dx.doi.org/10.1038/s41592-020-0835-7
Descripción
Sumario:Femtosecond lasers at fixed wavelengths above 1000 nm are powerful, stable, and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the Calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators.

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