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jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths
Femtosecond lasers at fixed wavelengths above 1000 nm are powerful, stable, and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335340/ https://www.ncbi.nlm.nih.gov/pubmed/32451475 http://dx.doi.org/10.1038/s41592-020-0835-7 |
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| author | Mohr, Manuel Alexander Bushey, Daniel Aggarwal, Abhi Marvin, Jonathan S. Kim, Jeong Jun Marquez, Emiliano Jimenez Liang, Yajie Patel, Ronak Macklin, John J. Lee, Chi-Yu Tsang, Arthur Tsegaye, Getahun Ahrens, Allison M. Chen, Jerry L. Kim, Douglas S. Wong, Allan M. Looger, Loren L. Schreiter, Eric R. Podgorski, Kaspar |
| author_facet | Mohr, Manuel Alexander Bushey, Daniel Aggarwal, Abhi Marvin, Jonathan S. Kim, Jeong Jun Marquez, Emiliano Jimenez Liang, Yajie Patel, Ronak Macklin, John J. Lee, Chi-Yu Tsang, Arthur Tsegaye, Getahun Ahrens, Allison M. Chen, Jerry L. Kim, Douglas S. Wong, Allan M. Looger, Loren L. Schreiter, Eric R. Podgorski, Kaspar |
| author_sort | Mohr, Manuel Alexander |
| collection | PubMed |
| description | Femtosecond lasers at fixed wavelengths above 1000 nm are powerful, stable, and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the Calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators. |
| format | Online Article Text |
| id | pubmed-7335340 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-73353402020-11-25 jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths Mohr, Manuel Alexander Bushey, Daniel Aggarwal, Abhi Marvin, Jonathan S. Kim, Jeong Jun Marquez, Emiliano Jimenez Liang, Yajie Patel, Ronak Macklin, John J. Lee, Chi-Yu Tsang, Arthur Tsegaye, Getahun Ahrens, Allison M. Chen, Jerry L. Kim, Douglas S. Wong, Allan M. Looger, Loren L. Schreiter, Eric R. Podgorski, Kaspar Nat Methods Article Femtosecond lasers at fixed wavelengths above 1000 nm are powerful, stable, and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the Calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators. 2020-05-25 2020-07 /pmc/articles/PMC7335340/ /pubmed/32451475 http://dx.doi.org/10.1038/s41592-020-0835-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Mohr, Manuel Alexander Bushey, Daniel Aggarwal, Abhi Marvin, Jonathan S. Kim, Jeong Jun Marquez, Emiliano Jimenez Liang, Yajie Patel, Ronak Macklin, John J. Lee, Chi-Yu Tsang, Arthur Tsegaye, Getahun Ahrens, Allison M. Chen, Jerry L. Kim, Douglas S. Wong, Allan M. Looger, Loren L. Schreiter, Eric R. Podgorski, Kaspar jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| title | jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| title_full | jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| title_fullStr | jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| title_full_unstemmed | jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| title_short | jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| title_sort | jycamp: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335340/ https://www.ncbi.nlm.nih.gov/pubmed/32451475 http://dx.doi.org/10.1038/s41592-020-0835-7 |
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