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Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, re...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335352/ https://www.ncbi.nlm.nih.gov/pubmed/32445620 http://dx.doi.org/10.1016/j.molcel.2020.04.026 |
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author | Kong, Muwen Cutts, Erin E. Pan, Dongqing Beuron, Fabienne Kaliyappan, Thangavelu Xue, Chaoyou Morris, Edward P. Musacchio, Andrea Vannini, Alessandro Greene, Eric C. |
author_facet | Kong, Muwen Cutts, Erin E. Pan, Dongqing Beuron, Fabienne Kaliyappan, Thangavelu Xue, Chaoyou Morris, Edward P. Musacchio, Andrea Vannini, Alessandro Greene, Eric C. |
author_sort | Kong, Muwen |
collection | PubMed |
description | Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells. |
format | Online Article Text |
id | pubmed-7335352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73353522020-11-16 Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA Kong, Muwen Cutts, Erin E. Pan, Dongqing Beuron, Fabienne Kaliyappan, Thangavelu Xue, Chaoyou Morris, Edward P. Musacchio, Andrea Vannini, Alessandro Greene, Eric C. Mol Cell Article Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells. Cell Press 2020-07-02 /pmc/articles/PMC7335352/ /pubmed/32445620 http://dx.doi.org/10.1016/j.molcel.2020.04.026 Text en © 2020 The Authors. Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kong, Muwen Cutts, Erin E. Pan, Dongqing Beuron, Fabienne Kaliyappan, Thangavelu Xue, Chaoyou Morris, Edward P. Musacchio, Andrea Vannini, Alessandro Greene, Eric C. Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA |
title | Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA |
title_full | Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA |
title_fullStr | Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA |
title_full_unstemmed | Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA |
title_short | Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA |
title_sort | human condensin i and ii drive extensive atp-dependent compaction of nucleosome-bound dna |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335352/ https://www.ncbi.nlm.nih.gov/pubmed/32445620 http://dx.doi.org/10.1016/j.molcel.2020.04.026 |
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