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Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA

Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, re...

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Autores principales: Kong, Muwen, Cutts, Erin E., Pan, Dongqing, Beuron, Fabienne, Kaliyappan, Thangavelu, Xue, Chaoyou, Morris, Edward P., Musacchio, Andrea, Vannini, Alessandro, Greene, Eric C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335352/
https://www.ncbi.nlm.nih.gov/pubmed/32445620
http://dx.doi.org/10.1016/j.molcel.2020.04.026
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author Kong, Muwen
Cutts, Erin E.
Pan, Dongqing
Beuron, Fabienne
Kaliyappan, Thangavelu
Xue, Chaoyou
Morris, Edward P.
Musacchio, Andrea
Vannini, Alessandro
Greene, Eric C.
author_facet Kong, Muwen
Cutts, Erin E.
Pan, Dongqing
Beuron, Fabienne
Kaliyappan, Thangavelu
Xue, Chaoyou
Morris, Edward P.
Musacchio, Andrea
Vannini, Alessandro
Greene, Eric C.
author_sort Kong, Muwen
collection PubMed
description Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells.
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spelling pubmed-73353522020-11-16 Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA Kong, Muwen Cutts, Erin E. Pan, Dongqing Beuron, Fabienne Kaliyappan, Thangavelu Xue, Chaoyou Morris, Edward P. Musacchio, Andrea Vannini, Alessandro Greene, Eric C. Mol Cell Article Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compaction of double-stranded DNA. Nucleosomes are incorporated into DNA loops during compaction without being displaced from the DNA, indicating that condensin complexes can readily act upon nucleosome-bound DNA molecules. These observations shed light on critical processes involved in genome organization in human cells. Cell Press 2020-07-02 /pmc/articles/PMC7335352/ /pubmed/32445620 http://dx.doi.org/10.1016/j.molcel.2020.04.026 Text en © 2020 The Authors. Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kong, Muwen
Cutts, Erin E.
Pan, Dongqing
Beuron, Fabienne
Kaliyappan, Thangavelu
Xue, Chaoyou
Morris, Edward P.
Musacchio, Andrea
Vannini, Alessandro
Greene, Eric C.
Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
title Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
title_full Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
title_fullStr Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
title_full_unstemmed Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
title_short Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA
title_sort human condensin i and ii drive extensive atp-dependent compaction of nucleosome-bound dna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335352/
https://www.ncbi.nlm.nih.gov/pubmed/32445620
http://dx.doi.org/10.1016/j.molcel.2020.04.026
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