Cargando…

Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma

Stomach adenocarcinoma (STAD) is one of the most common malignancies. But the molecular mechanism is unknown. In this study, we downloaded the transcriptional profiles and clinical data of 344 STAD and 30 normal samples from The Cancer Genome Atlas (TCGA) database. Stromal and immune scores of STAD...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Xinying, Xie, Xiaoli, Zhao, Qingchao, Zhang, Lixian, Li, Changjuan, Zhao, Dongqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335387/
https://www.ncbi.nlm.nih.gov/pubmed/32685487
http://dx.doi.org/10.1155/2020/4673153
_version_ 1783554126730231808
author Zhu, Xinying
Xie, Xiaoli
Zhao, Qingchao
Zhang, Lixian
Li, Changjuan
Zhao, Dongqiang
author_facet Zhu, Xinying
Xie, Xiaoli
Zhao, Qingchao
Zhang, Lixian
Li, Changjuan
Zhao, Dongqiang
author_sort Zhu, Xinying
collection PubMed
description Stomach adenocarcinoma (STAD) is one of the most common malignancies. But the molecular mechanism is unknown. In this study, we downloaded the transcriptional profiles and clinical data of 344 STAD and 30 normal samples from The Cancer Genome Atlas (TCGA) database. Stromal and immune scores of STAD were calculated by the Estimation of Stromal and Immune cells in Malignant Tumor tissues using Expression data (ESTIMATE) algorithm, and association of stromal/immune scores with tumor differentiation/T/N/M stage and survival was investigated. The differentially expressed genes (DEGs) between high and low score groups (based on media) were identified, and prognostic genes over-/underexpressed in both STAD and stromal/immune signature were retrieved. Furthermore, proportions of 22 infiltrating immune cells for the cohort from TCGA were estimated by the Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) algorithm, and association of 22 immune cells with tumor differentiation/T/N/M stage and survival was investigated. Next, coexpression analysis of 22 immune cells and intersection genes over-/underexpressed in both STAD and stromal signature was conducted. We found high stromal and immune scores and macrophage infiltration predicting poor tumor differentiation and severe local invasion, obtained a list of prognostic genes based on stromal-immune signature, and explored the interaction of collagen, chemokines such as CXCL9, CXCL10, and CXCL11, and macrophage through coexpression analysis and may provide novel prognostic biomarkers and immunotherapeutic targets for STAD.
format Online
Article
Text
id pubmed-7335387
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-73353872020-07-17 Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma Zhu, Xinying Xie, Xiaoli Zhao, Qingchao Zhang, Lixian Li, Changjuan Zhao, Dongqiang Biomed Res Int Research Article Stomach adenocarcinoma (STAD) is one of the most common malignancies. But the molecular mechanism is unknown. In this study, we downloaded the transcriptional profiles and clinical data of 344 STAD and 30 normal samples from The Cancer Genome Atlas (TCGA) database. Stromal and immune scores of STAD were calculated by the Estimation of Stromal and Immune cells in Malignant Tumor tissues using Expression data (ESTIMATE) algorithm, and association of stromal/immune scores with tumor differentiation/T/N/M stage and survival was investigated. The differentially expressed genes (DEGs) between high and low score groups (based on media) were identified, and prognostic genes over-/underexpressed in both STAD and stromal/immune signature were retrieved. Furthermore, proportions of 22 infiltrating immune cells for the cohort from TCGA were estimated by the Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) algorithm, and association of 22 immune cells with tumor differentiation/T/N/M stage and survival was investigated. Next, coexpression analysis of 22 immune cells and intersection genes over-/underexpressed in both STAD and stromal signature was conducted. We found high stromal and immune scores and macrophage infiltration predicting poor tumor differentiation and severe local invasion, obtained a list of prognostic genes based on stromal-immune signature, and explored the interaction of collagen, chemokines such as CXCL9, CXCL10, and CXCL11, and macrophage through coexpression analysis and may provide novel prognostic biomarkers and immunotherapeutic targets for STAD. Hindawi 2020-06-26 /pmc/articles/PMC7335387/ /pubmed/32685487 http://dx.doi.org/10.1155/2020/4673153 Text en Copyright © 2020 Xinying Zhu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhu, Xinying
Xie, Xiaoli
Zhao, Qingchao
Zhang, Lixian
Li, Changjuan
Zhao, Dongqiang
Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma
title Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma
title_full Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma
title_fullStr Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma
title_full_unstemmed Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma
title_short Potential Prognostic Value and Mechanism of Stromal-Immune Signature in Tumor Microenvironment for Stomach Adenocarcinoma
title_sort potential prognostic value and mechanism of stromal-immune signature in tumor microenvironment for stomach adenocarcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335387/
https://www.ncbi.nlm.nih.gov/pubmed/32685487
http://dx.doi.org/10.1155/2020/4673153
work_keys_str_mv AT zhuxinying potentialprognosticvalueandmechanismofstromalimmunesignatureintumormicroenvironmentforstomachadenocarcinoma
AT xiexiaoli potentialprognosticvalueandmechanismofstromalimmunesignatureintumormicroenvironmentforstomachadenocarcinoma
AT zhaoqingchao potentialprognosticvalueandmechanismofstromalimmunesignatureintumormicroenvironmentforstomachadenocarcinoma
AT zhanglixian potentialprognosticvalueandmechanismofstromalimmunesignatureintumormicroenvironmentforstomachadenocarcinoma
AT lichangjuan potentialprognosticvalueandmechanismofstromalimmunesignatureintumormicroenvironmentforstomachadenocarcinoma
AT zhaodongqiang potentialprognosticvalueandmechanismofstromalimmunesignatureintumormicroenvironmentforstomachadenocarcinoma