Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia

The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its...

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Autores principales: Abid, Salma, Ferjani, Sana, El Moussi, Awatef, Ferjani, Asma, Nasr, Mejda, Landolsi, Ichrak, Saidi, Karima, Gharbi, Hanène, Letaief, Hajer, Hechaichi, Aicha, Safer, Mouna, Ben Alaya, Nissaf Bouafif ep, Boubaker, Ilhem Boutiba-Ben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2020
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335417/
https://www.ncbi.nlm.nih.gov/pubmed/32768876
http://dx.doi.org/10.1016/j.diagmicrobio.2020.115125
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author Abid, Salma
Ferjani, Sana
El Moussi, Awatef
Ferjani, Asma
Nasr, Mejda
Landolsi, Ichrak
Saidi, Karima
Gharbi, Hanène
Letaief, Hajer
Hechaichi, Aicha
Safer, Mouna
Ben Alaya, Nissaf Bouafif ep
Boubaker, Ilhem Boutiba-Ben
author_facet Abid, Salma
Ferjani, Sana
El Moussi, Awatef
Ferjani, Asma
Nasr, Mejda
Landolsi, Ichrak
Saidi, Karima
Gharbi, Hanène
Letaief, Hajer
Hechaichi, Aicha
Safer, Mouna
Ben Alaya, Nissaf Bouafif ep
Boubaker, Ilhem Boutiba-Ben
author_sort Abid, Salma
collection PubMed
description The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific context.
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spelling pubmed-73354172020-07-06 Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia Abid, Salma Ferjani, Sana El Moussi, Awatef Ferjani, Asma Nasr, Mejda Landolsi, Ichrak Saidi, Karima Gharbi, Hanène Letaief, Hajer Hechaichi, Aicha Safer, Mouna Ben Alaya, Nissaf Bouafif ep Boubaker, Ilhem Boutiba-Ben Diagn Microbiol Infect Dis Virology The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific context. Elsevier Inc. 2020-11 2020-07-05 /pmc/articles/PMC7335417/ /pubmed/32768876 http://dx.doi.org/10.1016/j.diagmicrobio.2020.115125 Text en © 2020 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Virology
Abid, Salma
Ferjani, Sana
El Moussi, Awatef
Ferjani, Asma
Nasr, Mejda
Landolsi, Ichrak
Saidi, Karima
Gharbi, Hanène
Letaief, Hajer
Hechaichi, Aicha
Safer, Mouna
Ben Alaya, Nissaf Bouafif ep
Boubaker, Ilhem Boutiba-Ben
Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia
title Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia
title_full Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia
title_fullStr Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia
title_full_unstemmed Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia
title_short Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia
title_sort assessment of sample pooling for sars-cov-2 molecular testing for screening of asymptomatic persons in tunisia
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335417/
https://www.ncbi.nlm.nih.gov/pubmed/32768876
http://dx.doi.org/10.1016/j.diagmicrobio.2020.115125
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