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Renal cell tumors convert natural killer cells to a proangiogenic phenotype

Natural killer (NK) cells are classically associated with immune surveillance and destruction of tumor cells. Inconsistent with this function, NK cells are found in advanced human tumors including renal cell carcinoma (RCC). NK cells with non-classical phenotypes (CD56(+)CD16(dim/neg); termed decidu...

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Autores principales: Guan, Yue, Chambers, Christopher B., Tabatabai, Taylor, Hatley, Ha, Delfino, Kristin R., Robinson, Kathy, Alanee, Shaheen R., Ran, Sophia, Torry, Donald S., Wilber, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335666/
https://www.ncbi.nlm.nih.gov/pubmed/32655841
http://dx.doi.org/10.18632/oncotarget.27654
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author Guan, Yue
Chambers, Christopher B.
Tabatabai, Taylor
Hatley, Ha
Delfino, Kristin R.
Robinson, Kathy
Alanee, Shaheen R.
Ran, Sophia
Torry, Donald S.
Wilber, Andrew
author_facet Guan, Yue
Chambers, Christopher B.
Tabatabai, Taylor
Hatley, Ha
Delfino, Kristin R.
Robinson, Kathy
Alanee, Shaheen R.
Ran, Sophia
Torry, Donald S.
Wilber, Andrew
author_sort Guan, Yue
collection PubMed
description Natural killer (NK) cells are classically associated with immune surveillance and destruction of tumor cells. Inconsistent with this function, NK cells are found in advanced human tumors including renal cell carcinoma (RCC). NK cells with non-classical phenotypes (CD56(+)CD16(dim/neg); termed decidua NK (dNK) cells) accumulate at the maternal-fetal interface during embryo implantation. These dNK cells are poorly cytotoxic, proangiogenic, and facilitate placenta development. As similarities between embryo implantation and tumor growth exist, we tested the hypothesis that an analogous shift in NK cell phenotype and function occurs in RCC tumors. Our results show that peripheral NK (pNK) cells of RCC patients were uniformly CD56(+)CD16(bright), but lacked full cytotoxic ability. By comparison, RCC tumor-infiltrated NK (TiNK) cells were significantly enriched for CD56(+)CD16(dim-neg) cells, a phenotype of dNK cells. Gene expression analysis revealed that angiogenic and inflammatory genes were significantly increased for RCC TiNK versus RCC pNK populations, with enrichment of genes in the hypoxia inducible factor (HIF) 1α pathway. Consistent with this finding, NK cells cultured under hypoxia demonstrated limited cytotoxicity capacity, but augmented production of vascular endothelial growth factor (VEGF). Finally, comparison of gene expression data for RCC TiNK and dNK cells revealed a shared transcriptional signature of genes with known roles in angiogenesis and immunosuppression. These studies confirm conversion of pNK cells to a dNK-like phenotype in RCC tumors. These characteristics are conceivably beneficial for placentation, but likely exploited to support early tumor growth and promote metastasis.
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spelling pubmed-73356662020-07-10 Renal cell tumors convert natural killer cells to a proangiogenic phenotype Guan, Yue Chambers, Christopher B. Tabatabai, Taylor Hatley, Ha Delfino, Kristin R. Robinson, Kathy Alanee, Shaheen R. Ran, Sophia Torry, Donald S. Wilber, Andrew Oncotarget Research Paper Natural killer (NK) cells are classically associated with immune surveillance and destruction of tumor cells. Inconsistent with this function, NK cells are found in advanced human tumors including renal cell carcinoma (RCC). NK cells with non-classical phenotypes (CD56(+)CD16(dim/neg); termed decidua NK (dNK) cells) accumulate at the maternal-fetal interface during embryo implantation. These dNK cells are poorly cytotoxic, proangiogenic, and facilitate placenta development. As similarities between embryo implantation and tumor growth exist, we tested the hypothesis that an analogous shift in NK cell phenotype and function occurs in RCC tumors. Our results show that peripheral NK (pNK) cells of RCC patients were uniformly CD56(+)CD16(bright), but lacked full cytotoxic ability. By comparison, RCC tumor-infiltrated NK (TiNK) cells were significantly enriched for CD56(+)CD16(dim-neg) cells, a phenotype of dNK cells. Gene expression analysis revealed that angiogenic and inflammatory genes were significantly increased for RCC TiNK versus RCC pNK populations, with enrichment of genes in the hypoxia inducible factor (HIF) 1α pathway. Consistent with this finding, NK cells cultured under hypoxia demonstrated limited cytotoxicity capacity, but augmented production of vascular endothelial growth factor (VEGF). Finally, comparison of gene expression data for RCC TiNK and dNK cells revealed a shared transcriptional signature of genes with known roles in angiogenesis and immunosuppression. These studies confirm conversion of pNK cells to a dNK-like phenotype in RCC tumors. These characteristics are conceivably beneficial for placentation, but likely exploited to support early tumor growth and promote metastasis. Impact Journals LLC 2020-06-30 /pmc/articles/PMC7335666/ /pubmed/32655841 http://dx.doi.org/10.18632/oncotarget.27654 Text en http://creativecommons.org/licenses/by/3.0/ Copyright: Guan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Guan, Yue
Chambers, Christopher B.
Tabatabai, Taylor
Hatley, Ha
Delfino, Kristin R.
Robinson, Kathy
Alanee, Shaheen R.
Ran, Sophia
Torry, Donald S.
Wilber, Andrew
Renal cell tumors convert natural killer cells to a proangiogenic phenotype
title Renal cell tumors convert natural killer cells to a proangiogenic phenotype
title_full Renal cell tumors convert natural killer cells to a proangiogenic phenotype
title_fullStr Renal cell tumors convert natural killer cells to a proangiogenic phenotype
title_full_unstemmed Renal cell tumors convert natural killer cells to a proangiogenic phenotype
title_short Renal cell tumors convert natural killer cells to a proangiogenic phenotype
title_sort renal cell tumors convert natural killer cells to a proangiogenic phenotype
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335666/
https://www.ncbi.nlm.nih.gov/pubmed/32655841
http://dx.doi.org/10.18632/oncotarget.27654
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