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Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis

BACKGROUND: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. METHODS: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay...

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Autores principales: Li, Haoyi, Xia, Tian, Guan, Yilin, Yu, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335772/
https://www.ncbi.nlm.nih.gov/pubmed/32669871
http://dx.doi.org/10.2147/CMAR.S242135
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author Li, Haoyi
Xia, Tian
Guan, Yilin
Yu, Yao
author_facet Li, Haoyi
Xia, Tian
Guan, Yilin
Yu, Yao
author_sort Li, Haoyi
collection PubMed
description BACKGROUND: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. METHODS: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo. RESULTS: Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced glucose consumption and lactate production. Circ_0002755 was significantly upregulated in glioma tissues and cells, while its level was notably declined under Sev treatment. Besides, overexpression of circ_0002755 overturned Sev-mediated inhibitory effect on glioma progression. Further research indicated that circ_0002755 targeted miR-628-5p, and miR-628-5p targeted MAGT1, and Sev modulated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Moreover, Sev hindered tumor growth in vivo. CONCLUSION: Sev mediated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis.
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spelling pubmed-73357722020-07-14 Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis Li, Haoyi Xia, Tian Guan, Yilin Yu, Yao Cancer Manag Res Original Research BACKGROUND: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. METHODS: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo. RESULTS: Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced glucose consumption and lactate production. Circ_0002755 was significantly upregulated in glioma tissues and cells, while its level was notably declined under Sev treatment. Besides, overexpression of circ_0002755 overturned Sev-mediated inhibitory effect on glioma progression. Further research indicated that circ_0002755 targeted miR-628-5p, and miR-628-5p targeted MAGT1, and Sev modulated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Moreover, Sev hindered tumor growth in vivo. CONCLUSION: Sev mediated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Dove 2020-06-30 /pmc/articles/PMC7335772/ /pubmed/32669871 http://dx.doi.org/10.2147/CMAR.S242135 Text en © 2020 Li et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Li, Haoyi
Xia, Tian
Guan, Yilin
Yu, Yao
Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
title Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
title_full Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
title_fullStr Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
title_full_unstemmed Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
title_short Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
title_sort sevoflurane regulates glioma progression by circ_0002755/mir-628-5p/magt1 axis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335772/
https://www.ncbi.nlm.nih.gov/pubmed/32669871
http://dx.doi.org/10.2147/CMAR.S242135
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