Biophysical Parameters of GEVIs: Considerations for Imaging Voltage

Genetically encoded voltage indicators (GEVIs) continue to evolve, resulting in many different probes with varying strengths and weaknesses. Developers of new GEVIs tend to highlight their positive features. A recent article from an independent laboratory has compared the signal/noise ratios of a nu...

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Autores principales: Rhee, Jun Kyu, Leong, Lee Min, Mukim, Md Sofequl Islam, Kang, Bok Eum, Lee, Sungmoo, Bilbao-Broch, Laura, Baker, Bradley J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2020
Materias:
Biophysical Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335909/
https://www.ncbi.nlm.nih.gov/pubmed/32521239
http://dx.doi.org/10.1016/j.bpj.2020.05.019
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author Rhee, Jun Kyu
Leong, Lee Min
Mukim, Md Sofequl Islam
Kang, Bok Eum
Lee, Sungmoo
Bilbao-Broch, Laura
Baker, Bradley J.
author_facet Rhee, Jun Kyu
Leong, Lee Min
Mukim, Md Sofequl Islam
Kang, Bok Eum
Lee, Sungmoo
Bilbao-Broch, Laura
Baker, Bradley J.
author_sort Rhee, Jun Kyu
collection PubMed
description Genetically encoded voltage indicators (GEVIs) continue to evolve, resulting in many different probes with varying strengths and weaknesses. Developers of new GEVIs tend to highlight their positive features. A recent article from an independent laboratory has compared the signal/noise ratios of a number of GEVIs. Such a comparison can be helpful to investigators eager to try to image the voltage of excitable cells. In this perspective, we will present examples of how the biophysical features of GEVIs affect the imaging of excitable cells in an effort to assist researchers when considering probes for their specific needs.
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spelling pubmed-73359092020-10-10 Biophysical Parameters of GEVIs: Considerations for Imaging Voltage Rhee, Jun Kyu Leong, Lee Min Mukim, Md Sofequl Islam Kang, Bok Eum Lee, Sungmoo Bilbao-Broch, Laura Baker, Bradley J. Biophys J Biophysical Perspective Genetically encoded voltage indicators (GEVIs) continue to evolve, resulting in many different probes with varying strengths and weaknesses. Developers of new GEVIs tend to highlight their positive features. A recent article from an independent laboratory has compared the signal/noise ratios of a number of GEVIs. Such a comparison can be helpful to investigators eager to try to image the voltage of excitable cells. In this perspective, we will present examples of how the biophysical features of GEVIs affect the imaging of excitable cells in an effort to assist researchers when considering probes for their specific needs. The Biophysical Society 2020-07-07 2020-05-29 /pmc/articles/PMC7335909/ /pubmed/32521239 http://dx.doi.org/10.1016/j.bpj.2020.05.019 Text en © 2020 Biophysical Society. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biophysical Perspective
Rhee, Jun Kyu
Leong, Lee Min
Mukim, Md Sofequl Islam
Kang, Bok Eum
Lee, Sungmoo
Bilbao-Broch, Laura
Baker, Bradley J.
Biophysical Parameters of GEVIs: Considerations for Imaging Voltage
title Biophysical Parameters of GEVIs: Considerations for Imaging Voltage
title_full Biophysical Parameters of GEVIs: Considerations for Imaging Voltage
title_fullStr Biophysical Parameters of GEVIs: Considerations for Imaging Voltage
title_full_unstemmed Biophysical Parameters of GEVIs: Considerations for Imaging Voltage
title_short Biophysical Parameters of GEVIs: Considerations for Imaging Voltage
title_sort biophysical parameters of gevis: considerations for imaging voltage
topic Biophysical Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335909/
https://www.ncbi.nlm.nih.gov/pubmed/32521239
http://dx.doi.org/10.1016/j.bpj.2020.05.019
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